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The Agtrap is a laboratory instrument designed for the capture and isolation of analytes. It utilizes a specialized trapping mechanism to selectively retain target molecules from complex samples. The core function of the Agtrap is to facilitate the extraction and purification of specific analytes for further analysis.

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2 protocols using agtrap

1

Quantitative RT-PCR Analysis of Kidney Genes

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Quantitative real-time reverse transcriptase–PCR was performed using designed TaqMan probes (Agtrap, Mm00507771_m1; AT1R, Mm01957722_s1; NHE3, Mm01352473_m1; NKCC2, Mm01275821_m1; NCC, Mm00490213_m1; αENaC, Mm00803386_m1; βENaC, Mm00441215_m1; γENaC, Mm00441228_m1; and AGT, Mm00599662_m1; Applied Biosystems, Carlsbad, CA), as described previously.8 (link) The mRNA levels were normalized to those of the 18S rRNA control.
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2

Real-Time RT-qPCR Analysis of Kidney Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Quantitative real-time reverse transcriptase–PCR was performed using designed TaqMan probes (Agtrap, Mm00507771_m1; AT1R, Mm01957722_s1; NHE3, Mm01352473_m1; NKCC2, Mm01275821_m1; NCC, Mm00490213_m1; αENaC, Mm00803386_m1; βENaC, Mm00441215_m1; γENaC, Mm00441228_m1; and AGT, Mm00599662_m1; Applied Biosystems, Carlsbad, CA), as described previously.8 The mRNA levels were normalized to those of the 18S rRNA control.
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