Mda mb 468 breast

MDA-MB-231, BT549, MDA-MB-468 breast cancer cells, 293T, 143B (human osteosarcoma cells), SKOV3 (human ovarian cancer cells), DU145 (human prostate cancer cells), and SW1990 (human pancreatic cancer cells) were purchased from ATCC (Manasseh's, VA, USA). Cells were maintained in L-15 or in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. MDA-MB-231-LUC was cultured in Minimum Essential Medium supplemented with 10% FBS and non-essential amino acids^{39 (link)}. HUVECs (ScienCell Research Laboratories, San Diego, CA, USA) were purchased from Science Research Laboratories and cultured in complete ECM (ScienCell) supplemented with 5% FBS. CHO cells were maintained in DMEM supplemented with 10% FBS, 2 mM L-glutamine, 1 mM sodium pyruvate, and 100 μM non-essential amino acids. All cells were maintained at 37 °C under a humidified 5% CO₂ incubator. All cell lines were periodically monitored for mycoplasma contamination.

BT-20, MDA-MB-468 breast cancer cells, and BJ-5ta were purchased from ATCC and cultured in DMEM medium supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 1% penicillin/streptomycin. For the cytotoxicity assay, cells were seeded at low density in 96-well plates in serine containing media following a previously reported procedure [25 (link)]. Media was then aspirated, and cells were incubated in fresh serine-replete or -deplete media containing compounds at indicated concentrations or vehicle (DMSO). Cells were grown for 5 days at 37 °C with drug and media changed daily before assaying cell viability using a Presto Blue assay (Life Technologies) according to the manufacturer’s instructions.