Proquant software

Thin layer chromatography (TLC) and TLC-DPPH analysis was performed for detection of flavonoids in the crude alcoholic extract and FE fraction using quercetin, kaempferol, and gallic acid standards. Silica Gel 60 F₂₅₄ precoated plates were used for analysis, and solvent system used was as previously described.[17 ] One percent Natural product reagent was used as derivatizing reagent and plates were observed under UV (366 nm) as previously reported.[17 18 (link)19 ] Another derivatizing reagent used was 0.1% DPPH and plates were observed under visible light.[20 (link)] High-performance thin layer chromatography (HPTLC) was performed for the FE fraction using DESAGA HPTLC system. The chromatograms were scanned at 420 nm after derivatization with 1% natural product reagent. The spectra and retention factor (Rf) values were recorded using ProQuant software (Informer Technologies, Inc.).

Detection of flavonoids from the EA fraction, water fraction, and crude alcoholic extract was performed by thin layer chromatography (TLC), TLC-DPPH (TLC-DPPH), and high-performance TLC (HPTLC) analysis using rutin as a standard. Samples were spotted on Silica Gel 60 F₂₅₄ precoated plates and the analysis was carried out using the standardized solvent system of EA:formic acid:glacial acetic acid:methanol (7.5:0.15:0.15:0.9). Plates were derivatized using 1% natural product reagent and observed under UV light at 366 nm.[17 18 (link)19 ] 0.1% DPPH was also used as a derivatizing reagent to detect antioxidant potential qualitatively.[20 (link)] EA fraction of okra was subjected to HPTLC analysis using DESAGA HPTLC system. The chromatograms were scanned, and the spectra and retention factor (Rf) were recorded using ProQuant software (Informer Technologies, Inc.).