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Fitc annexin 5 and pe pi apoptosis detection kit

Manufactured by BD
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Sourced in United States

The FITC-Annexin V and PE-PI apoptosis detection kit is a laboratory tool used to detect and quantify apoptosis, a type of programmed cell death. The kit contains FITC-conjugated Annexin V and propidium iodide (PE-PI) dyes, which bind to specific cellular components that are exposed during the apoptotic process. This allows researchers to analyze and differentiate between viable, early apoptotic, late apoptotic, and necrotic cells using flow cytometry or fluorescence microscopy.

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Lab products found in correlation

C6 flow cytometer, by BD (1 mentions) Accuri c6, by BD (1 mentions)

Close spelling variants of this product

Annexin V-FITC and PI apoptosis detection kit Annexin V-FITC and PI apoptosis kit Annexin V and PI apoptosis detection kit Annexin V-FITC and PI detection kit FITC Annexin V Apoptosis Detection Kit Annexin V-FITC and PI kit Annexin V and PI Detection Kit FITC Annexin V Apoptosis Detection Annexin V-FITC and PI PE Annexin V Apoptosis Detection

2 protocols using fitc annexin 5 and pe pi apoptosis detection kit

1

Quantifying Tumor Cell Apoptosis by Flow Cytometry

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Cell apoptosis was examined using the FITC-Annexin V and PE-PI apoptosis detection kit (BD, NJ, USA). Briefly, pre-treated tumor cells were collected and stained with FITC-Annexin V and PE-PI staining solution for 15 min at room temperature. Cell apoptosis was then detected by flow cytometry on a C6 flow cytometer (BD, NJ, USA). Each experiment was performed three times independently.
Zhao X., Liu C., He X., Wang M., Zhang H., Cheng J, & Wang H. (2022). Laminin-bound integrin α6β4 promotes non-small cell lung cancer progression via the activation of YAP/TAZ signaling pathway. Frontiers in Oncology, 12, 1015709.
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2

Quantitative Analysis of Apoptosis

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Cells apoptosis was analyzed using the FITC-Annexin V and PE-PI apoptosis detection kit (BD, NJ, USA). Briefly, tumor cells were collected and stained with FITC-Annexin V and PE-PI viability staining solution for 15 min. Subsequently, apoptosis was detected by flow cytometry on flow cytometer (Accuri®C6, Becton Dickinson, NJ, USA). Each experiment was performed three times, independently.
Lv Y., Shan Y., Song L., Zhao Y., Lai R., Su J, & Zhang X. (2021). Type I collagen promotes tumor progression of integrin β1 positive gastric cancer through a BCL9L/β-catenin signaling pathway. Aging (Albany NY), 13(14), 19064-19076.
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