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Rabbit cct3 polyclonal antiserum

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Rabbit CCT3 polyclonal antiserum is a laboratory reagent produced by immunizing rabbits with a specific antigen related to the CCT3 protein. This antiserum can be used for the detection and analysis of the CCT3 protein in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry.

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Lab products found in correlation

Lsm 710, by Zeiss (4 mentions) Lionheart fx automated microscope, by Agilent Technologies (2 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (2 mentions)

2 protocols using rabbit cct3 polyclonal antiserum

1

Confocal Microscopy for Reovirus σ3 Quantification

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Confocal microscopy was performed as described48 (link) with the following modifications: cells were fixed in 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15712-s) diluted in PBS−/− at RT for 20 min and counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, D3571) to label nuclei. Confocal images were captured using a Zeiss LSM 710 laser scanning confocal microscope equipped with a 63X oil objective. Images were processed using Zen 2012 and ImageJ software with the Fiji plugin55 (link). Immunofluorescence images to quantify folded and unfolded reovirus σ3 were captured using a Lionheart FX automated microscope (BioTek) equipped with a 20X air objective. Images were processed and signals quantified using Gen5+ software (BioTek). The following antibodies were used: guinea pig σNS polyclonal antiserum57 (link), mouse 10C1 σ3 monoclonal antibody (conformation-specific antibody, used to label native σ3)32 (link), rabbit VU219 σ3 polyclonal antiserum (used to label total σ3), rabbit anti-CCT1 polyclonal antiserum (NeoScientific, A1950), rabbit CCT3 polyclonal antiserum (ABclonal, A6547).
Knowlton J.J., De Castro I.F., Ashbrook A.W., Gestaut D.R., Zamora P.F., Bauer J.A., Forrest J.C., Frydman J., Risco C, & Dermody T.S. (2018). The TRiC Chaperonin Controls Reovirus Replication through Outer-Capsid Folding. Nature microbiology, 3(4), 481-493.
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2

Confocal Microscopy for Reovirus σ3 Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Confocal microscopy was performed as described48 (link) with the following modifications: cells were fixed in 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15712-s) diluted in PBS−/− at RT for 20 min and counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, D3571) to label nuclei. Confocal images were captured using a Zeiss LSM 710 laser scanning confocal microscope equipped with a 63X oil objective. Images were processed using Zen 2012 and ImageJ software with the Fiji plugin55 (link). Immunofluorescence images to quantify folded and unfolded reovirus σ3 were captured using a Lionheart FX automated microscope (BioTek) equipped with a 20X air objective. Images were processed and signals quantified using Gen5+ software (BioTek). The following antibodies were used: guinea pig σNS polyclonal antiserum57 (link), mouse 10C1 σ3 monoclonal antibody (conformation-specific antibody, used to label native σ3)32 (link), rabbit VU219 σ3 polyclonal antiserum (used to label total σ3), rabbit anti-CCT1 polyclonal antiserum (NeoScientific, A1950), rabbit CCT3 polyclonal antiserum (ABclonal, A6547).
Knowlton J.J., De Castro I.F., Ashbrook A.W., Gestaut D.R., Zamora P.F., Bauer J.A., Forrest J.C., Frydman J., Risco C, & Dermody T.S. (2018). The TRiC Chaperonin Controls Reovirus Replication through Outer-Capsid Folding. Nature microbiology, 3(4), 481-493.
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