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Pxi 1033

Manufactured by National Instruments
Sourced in United States

The PXI 1033 is a chassis module that provides a high-performance, modular platform for developing and deploying automated test and measurement systems. It offers a rugged and scalable design to accommodate a variety of PXI and PXI Express modules.

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3 protocols using pxi 1033

1

Automated Electrochemical Measurement System

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All of the electrochemical measurements used here were performed by an automated system, similar to one described previously [11 (link),16 (link),18 (link)]. This system consists of a: (a) PXI 1033: a 5-slot PXI chassis with an integrated MXI-Express controller (NI™, Austin, TX, USA); (b) PXI 4071: a 7.5-digit digital multimeter with a voltage measurement range of 10 nV to 1000 V and with an input resistance of more than 1010 Ω (NI™, Austin, TX, USA); (c) PXI 4022: a high-speed, high-precision guard, and current amplifier that can detect picoampere current levels with femtoampere noise with the PXI 4071 (NI™, Austin, TX, USA); and (d) PXI 2535: a high-density FET (NI™, Austin, TX, USA) switch matrix module that features 544 cross-points, 4 × 136 one-wire matrix configuration (136 channels), switching speeds as high as 50,000 cross-points/s, and unlimited simultaneous connection. More channels can be achieved via the facile expansion of switch modules (NI™, Austin, TX, USA). This device is directly controlled by a computer with a transparent, high-speed serial link [18 (link)].
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2

Interfacing Headstage with NI DAQ System

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We interfaced our headstage with National Instruments data acquisition systems using a custom interface PCB that provided regulated power and converted the multiplexer command signals from 5V to 2.5V logic (figure 1(c)). A low-noise bi-polar supply for the multiplexed headstage was generated by first inverting the 5V onboard power from the NI system to −5V using a charge pump voltage inverter (TPS60403DBVR, Texas Instruments) and then using a pair of ultra-low noise positive and negative linear regulators to provide regulated ±2.5V (TPS7A4901DGNR and TPS7A3001DGNR, Texas Instruments). The interface PCB was connected to a multicard PXI system (PXI-1033 or PXIe-1073, National Instruments) with 18-bit analog to digital converter cards (PXI-6289). However, a USB-6289 or any M-series or X-series DAQ system would also be compatible. Custom LabVIEW data acquisition software was used to control the acquisition and perform real-time demultiplexing, display and recording. We have previously published this software with an open source license [17 ].
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3

TIRF Imaging of Fluorescent Proteins

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TIRF imaging was carried out on a Nikon Eclipse Ti2 inverted microscope with a CFI60 60× Apo TIRF objective and a Hamamatsu Orca-Flash 4.0 V2 sCMOS camera. eGFP and Tag.RFP-T fluorescence was excited using 488 and 561 nm lasers and detected using a Chroma HC TIRF Quad Dichroic (C-FL TIRF Ultra Hi S/N 405/488/561/638) and Chroma HC Quad emission filters BP 525/50 and BP600/50, respectively (Bellows Falls, VT). Unless mentioned specifically, channels were acquired sequentially at a 1.2 s interval and 400 ms exposure time over 4.8 to 6 min. Real-time acquisition was achieved by a National Instruments (PXI 1033; Austin, TX) controller. The system was controlled with NIS-Elements software and maintained at 37°C by an OkoLab environmental chamber (Burlingame, CA).
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