Ophiopogonin B (MW: 722.90238 g/mol, purity ≥ 98%) was provided by Nanjing Ze Lang Medical Technology Co., Ltd (Jiangsu, China). The stock was prepared by dissolving the compound in dimethyl sulfoxide (DMSO, Sigma, US). The stock was frozen at -20°C. Culture medium was used to dilute OP-B to suitable doses before use, while the DMSO final concentration was less than 0.01%. Primary antibodies anti-β-catenin (8480), anti-phospho-β-catenin (Ser33/37/Thr4) (9561), anti-cyclin D1 (2978), anti-c-Myc (5605), anti-E-cadherin (14472), anti-vimentin (5741), anti-snail (3879), anti-slug (9585) and anti-LainB1 (13435) were provided by Cell Signaling Technology. Meanwhile, anti-β-actin antibody (sc4778) was provided by Santa Cruz Biotechnology. Anti-Axin1 (ab233652) and anti-GSK3β (ab93926) antibodies were purchased from Abcam. Goat anti-rabbit and anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibodies were provided by Cell Signaling Technology.
Anti β actin antibody sc4778
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The Anti-β-actin antibody (sc4778) is a primary antibody that recognizes the β-actin protein. β-actin is a widely expressed cytoskeletal protein that plays a crucial role in various cellular processes. This antibody can be used to detect and quantify β-actin expression in different samples and experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
G box imaging system, by Syngene (1 mentions)
Westernbright sirius western blotting detection kit, by Advansta (1 mentions)
Genesys software, by Syngene (1 mentions)
Ab109183, by Abcam (1 mentions)
Ab15148, by Abcam (1 mentions)
Ab109250, by Abcam (1 mentions)
Ab129473, by Abcam (1 mentions)
Ab126783, by Abcam (1 mentions)
Ab175430, by Abcam (1 mentions)
Ab97959, by Abcam (1 mentions)
Wnt3a, by R&D Systems (1 mentions)
Ab180714, by Abcam (1 mentions)
3 protocols using anti β actin antibody sc4778
1
Ophiopogonin B Inhibits Wnt/β-Catenin Signaling
2
HIV-1 p24 Gag Protein Detection
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Virus producer cells were lysed in 1× SDS loading buffer containing 5% 2-mercaptoethanol. Purified virions were diluted in an equal volume of 2× SDS loading buffer containing 5% 2-mercaptoethanol. p24 was detected by Western blotting with an anti-HIV-1 p24 Gag mouse monoclonal antibody (31 (link)) (catalog no. 6458; NIH AIDS Reagent Program). Expression of the β-actin loading control was detected with anti-β-actin antibody sc-4778 (Santa Cruz). After being probed with the primary antibodies, the blots were washed and incubated with anti-mouse IgG–horseradish peroxidase (A9044; Sigma-Aldrich). The membranes were washed again and incubated with the WesternBright Sirius Western blotting detection kit (K-12043-D20; Advansta). Chemiluminescence was visualized with the G:BOX Imaging System and GeneSys software (Syngene). p24 and β-actin levels were then quantified with GeneTools (Syngene)
3
Molecular Mechanisms of Epithelial-Mesenchymal Transition
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Anti-NEK2 antibodies (610593) for used in western blot analysis and immunofluorescence microscopywere purchased from BD Biosciences (San Jose, CA, USA) and those (sc-55601) for performing NEK2 kinase assay were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-ALDH1 antibodies (611194) were acquired from BD Biosciences. Antibodies against Smad2/3 (#5678), phosphorylated Smad2 (#3108), β-catenin (#9562), and phosphorylated β-catenin (Ser33/Ser37/Thr41, Ser45, or Ser675; #9561, #9564, or #4176, respectively) were purchased from Cell Signaling Biotechnology (Danvers, MA, USA). Antibodies against E-cadherin (ab15148), N-cadherin (ab18203), vimentin, Snail (ab180714), Twist (ab175430), Bmi1 (ab126783), Sox2 (ab97959), octamer-binding transcription factor 4 (Oct4; ab109183), Kruppel-like factor 4 (Klf4; ab129473) and Nanog (ab109250) were obtained from Abcam (Cambridge, MA, USA). Anti-β-actin antibody (sc-4778) and anti-Sp1 antibody (sc-17824) were obtained from Santa Cruz Biotechnology, and wortmannin, MG132, H89, PP2 and bisindolylmaleimide I (BMI) were purchased from Calbiochem (San Diego, CA, USA). Wnt3a was purchased from R&D Systems Inc. (Minneapolis, MN, USA). CGK062 was prepared as previously described (23 (link)).
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