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2 protocols using nabh4

1

Synthesis of Multicomponent Nanoparticles

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Iron(III) acetylacetonate (Fe(acac)3), 1,2-hexadecanediol, oleylamine, polyethylene glycol (PEG), oleic acid, benzyl ether, cetyl trimethyl ammonium bromide (CTAB), HAuCl4, NaBH4, l-ascorbic acid, agar and doxorubicin (DOX) were purchased from J&K Scientific Ltd. Glycolide and Lactide were purchased from Purac (Netherlands) and further purified by recrystallization from ethyl acetate for several times. Stannous octoate, polyvinyl alcohol (PVA) and fluorescein diacetate were purchased from Sigma Aldrich. Fast membrane emulsification equipment (FMEM-500 M) and Shirasu porous glass (SPG) membrane were purchased from National Engineering Research Center for Biotechnology (Beijing). The glass membranes were annular cylinders (inner diameter = 8 mm, external diameter = 10 mm, length = 170 mm) with pore sizes of 7.2 μm. All other reagents were purchased from Beijing Chemical Reagents Company and used as received without further purification. Cells were supplied by China Infrastructure of Cell Line Resources.
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2

Synthesis and Functionalization of Graphene-Based Nanomaterials

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Natural graphite flake (99.8% purity) was purchased from Sigma–Aldrich. Chloroauric acid (HAuCl4·3H2O, ≥49.0% Au basis), disodium hydrogen phosphate (Na2HPO4), sodium dihydrogen phosphate (NaH2PO4), phosphoric acid (H3PO4), sulfuric acid (H2SO4), HCl, NaBH4, potassium permanganate (KMnO4), BSA, ClCH2COONa, N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), and N-hydroxysuccinimide (NHS) were purchased from J&K company (Beijing, China). Ethanol was purchased from Beijing Chemicals Co., Ltd. (Beijing, China). All the chemicals used are at least of analytical grade and directly used without additional purification. The water purified through a Millipore system (18.2 MΩ·cm) was used throughout.
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