Dusp6 ab76310

Manufactured by Abcam

Sourced in Canada

DUSP6 (ab76310) is a lab equipment product offered by Abcam. It is a protein that functions as a dual-specificity phosphatase, capable of dephosphorylating both tyrosine and serine/threonine residues on its target proteins.

Automatically generated - may contain errors

Lab products found in correlation

α tubulin, by Merck Group (1 mentions) Gapdh g9545, by Merck Group (1 mentions) Alexa fluor conjugate antibodies, by Thermo Fisher Scientific (1 mentions) Phospho erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Actinomycin d, by Merck Group (1 mentions) Tcf1 2203 s, by Cell Signaling Technology (1 mentions) Sc 47778 hrp, by Santa Cruz Biotechnology (1 mentions) Cd45ra pe cy7, by BioLegend (1 mentions) Sb216763 s3442, by Merck Group (1 mentions) Cd69 percp cy5.5 560738, by BioLegend (1 mentions) Cd62l pe 304806, by BioLegend (1 mentions) Alexa fluor 488 conjugated antibody, by Thermo Fisher Scientific (1 mentions) Ki67 gtx16667, by GeneTex (1 mentions) Erk2 sc 154, by Santa Cruz Biotechnology (1 mentions) Alkaline phosphatase conjugated antibodies, by Promega (1 mentions) β actin mab1501r, by Merck Group (1 mentions)

3 protocols using dusp6 ab76310

Protein Expression Regulation Study

Check if the same lab product or an alternative is used in the 5 most similar protocols

The commercial antibodies used in the study were raised against: P2RX7 (PR-004, Alomone Laboratories); Sp1 (#07-645, Merck Millipore); SAPK/JNK (#9252), anti-phospho-SAPK/JNK (Thr183/Tyr185) (#4668), p38 (#9212), phospho-p38 (Thr180/Tyr182) (#4511) and phospho-Erk1/2 (Thr202/Tyr204) (#4370), all from Cell Signaling; Erk (sc-1647) and DUSP1 (sc-370) from Santa Cruz Biotechnology; DUSP6 (ab76310) from Abcam; GAPDH (G9545) and α-tubulin (T5168) from Sigma-Aldrich. The horseradish peroxidase-conjugated secondary antibodies were from Dako (P0448 and P0260) and the secondary Alexa Fluor^® conjugate antibodies (A31572 and A11001) were from Molecular Probes.
The specific inhibitors of MAPKs and other proteins used were: SP600125 (JNK), SB202190 (p38), U0126 (MEK), 666-15 (CREB), SR11302 (AP-1), KJ Pyr 9 (Myc), cyclic Pifithrin α (p53) and VO-OH Pic (PTEN), all from Tocris Bioscience; BCI (DUSP1/DUSP6), okadaic acid (PP2A) and BBG (P2RX7) were supplied by Merck-Millipore; mithramycin A (Sp1) and actinomycin D were purchased from Sigma-Aldrich. Other chemicals were supplied by Merck Millipore or Sigma-Aldrich.

Benito-León M., Gil-Redondo J.C., Perez-Sen R., Delicado E.G., Ortega F, & Gomez-Villafuertes R. (2022). BCI, an inhibitor of the DUSP1 and DUSP6 dual specificity phosphatases, enhances P2X7 receptor expression in neuroblastoma cells. Frontiers in Cell and Developmental Biology, 10, 1049566.

+ Open protocol

+ Expand

Immunoblotting and ChIP-PCR Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies specific for TCF1 (#2203 S) and pERK (T202/Y204, #4377 S) from Cell Signaling Technology, and DUSP6 (ab76310) from Abcam, were used at a dilution of 1 in 1000 for immunoblotting or 1 in 50 for ChIP-PCR. Antibodies specific for β-actin were from Santa Cruz Biotechnology (sc-47778 HRP) and used at 1 in 10000. Fluorochrome-conjugated antibodies were from BD Biosciences including CD4-APC (555349), CD69- PerCP-Cy5.5 (560738) and CD45RA-PE-Cy™7 and BioLegend including CD62L-PE (304806), CD25-APC (302610) and human IL-2 Alexa-Fluor-488 (500314). All antibodies for flow cytometry were used at 1 in 50. PIM1 inhibitor (526521), GSK3β inhibitors, BIO (2’Z,3’E)-6-Bromoindirubin-3ʹ-oxime (361550) and SB216763 (S3442), were from Sigma.

Ye Z., Gould T.M., Zhang H., Jin J., Weyand C.M, & Goronzy J.J. (2021). The GSK3β-β-catenin-TCF1 pathway improves naive T cell activation in old adults by upregulating miR-181a. NPJ Aging and Mechanisms of Disease, 7, 4.

+ Open protocol

+ Expand

Immunoblotting and Immunofluorescence Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary antibodies were obtained from the following sources: DUSP6 (ab76310) from Abcam (Toronto, Canada), β‐actin (MAB1501R) from Millipore (Oakville, Canada), ERK2 (sc‐154) and MEK2 (sc‐524) from Santa Cruz Biotechnology (Santa Cruz, CA), phosphorylated ERK1/2 (M8159) from Sigma‐Aldrich (Oakville, Canada), phosphorylated MEK1/2 (9121) from Cell Signaling Technology (Danvers, MA, USA), chromogranin A (20085) from ImmunoStar (Hudson, WI), proliferating cell nuclear antigen (PCNA; 18197) from Abcam and Ki67 (GTX16667) from Genetex (Irvine, CA). Horseradish peroxidase antibodies were obtained from GE Healthcare Life Sciences (Mississauga, Canada) and alkaline phosphatase‐conjugated antibodies from Promega (Madison, WI). For immunofluorescence, Alexa Fluor 488 conjugated antibodies were obtained from Molecular Probes (Waltham, MA). Other materials were purchased from Sigma‐Aldrich unless stated otherwise.

Beaudry K., Langlois M., Montagne A., Cagnol S., Carrier J.C, & Rivard N. (2018). Dual‐specificity phosphatase 6 deletion protects the colonic epithelium against inflammation and promotes both proliferation and tumorigenesis. Journal of Cellular Physiology, 234(5), 6731-6745.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!