Immunofluorescence analysis was performed as previously described 68 (link) using the primary and secondary antibodies detailed above and followed by 10 min. incubation with DAPI (1/500). Coverslips were mounted with Fluoromount-G according to the provider's guidelines. Representative images were taken with an Axiovert200 (Zeiss, Oberkochen, Germany) inverted microscope coupled to a CCD camera using Metavue 5.07 software (Universal Imaging, Bedford Hills, NY, USA).
Metavue 5
Manufactured by Universal Imaging
Sourced in United States
The Metavue 5.07 is a versatile and advanced microscope system designed for a wide range of laboratory applications. It features a high-resolution optical system that delivers clear and detailed images, enabling users to conduct detailed analysis and observations. The Metavue 5.07 is equipped with various features and functionalities to support diverse research and investigation needs.
Automatically generated - may contain errors
3 protocols using metavue 5
1
Immunofluorescence Microscopy Protocol
2
Mounting Embryos for In Situ Hybridization
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Fixed embryos processed for in situ hybridization were mounted on Rain-X coated (ITW Global Brands, Houston, TX) glass slides in 80% glycerol/20% 1X PBS. Coverslips were prepared as described in Lyons et al. [62 (link)] for C. fornicata and with two additional supporting coverslips at the sides for L. gigantea. All images were acquired using an Axioskop 2 plus microscope (Zeiss) in conjunction with a CoolSnap FX color camera (Roper Scientific) and MetaVue 5.07 (Universal Imaging) software from Centro de Biología Molecular Severo Ochoa SMOC facilities. Additional image processing was done with Helicon Focus (Helicon Soft Ltd. Kharkov, Ukraine) to combine multifocal stacks of images and get focused images and Adobe Photoshop (Adobe Systems Software Ireland Ltd.) for the stacking of bright-field and fluorescent images of C. fornicata embryos.
3
Fluorescence Microscopy Imaging Workflow
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Analysis and photography of fluorescent cultures were done using a vertical fluorescence microscope Axioskop2 plus (Zeiss) coupled to a Coolsnap FX color camera navigated by Metavue 5.07 (Universal Imaging) operated by Adobe Photoshop 5.0 (Pantone Inc). Images have been processed using the Adobe Design Premium CS5.
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