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Mda mb 134 6

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The MDA-MB-134-VI is a human breast cancer cell line. It is a well-characterized and widely used cell line for research purposes.

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Lab products found in correlation

Mcf 7, by American Type Culture Collection (5 mentions) Mda mb 231, by American Type Culture Collection (2 mentions) Zr 75 1, by American Type Culture Collection (2 mentions) Bt474, by American Type Culture Collection (2 mentions) Mda mb 468, by American Type Culture Collection (2 mentions) Hcc1143, by American Type Culture Collection (2 mentions) Mda mb 453, by American Type Culture Collection (2 mentions) Du4475, by American Type Culture Collection (2 mentions) Powerplex 1.2 system, by Promega (1 mentions) Au565, by American Type Culture Collection (1 mentions) Dmem medium, by Corning (1 mentions) Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions) Hepes, by Thermo Fisher Scientific (1 mentions) Gentamicin, by Thermo Fisher Scientific (1 mentions) Insulin, by Merck Group (1 mentions) Hydrocortisone, by Merck Group (1 mentions) Transferrin, by Merck Group (1 mentions) Mycoalert detection kit, by Lonza (1 mentions) Fungizone, by Merck Group (1 mentions) Mia paca 2, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

MDA-MB-134 (5 citations) MDA-MB-134-VI (MM134), (3 citations)

6 protocols using mda mb 134 6

1

Breast Cancer Cell Line Establishment

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MDA-MB134-VI, MCF7, and T47D were purchased from ATCC (LGC Standards, Teddington, UK), while SUM44 was purchased from Asterand (Royston, UK). Cells were cultured at 37°C in a 5% CO2 atmosphere in phenol-free RPMI medium (MDA-MB134-VI and T47D) or DMEM (MCF7 and T47D) supplemented with 10% fetal bovine serum and 1nM 17β-estradiol (E2). Prior to experimentation, cell lines were stripped of estrogen by culturing for 24 hours in their appropriate medium containing 10% dextran charcoal-stripped fetal bovine serum (DCC) as previously described [28 (link)]. The generation of the long-term estrogen deprived (LTED) cell line derivative of MCF7 has been previously described [29 (link)]. All cell lines were shown to be mycoplasma-free and authenticated by means of short tandem repeat analysis (PowerPlex® 1.2 System, Promega, Fitchburg, WI, USA).
López-Knowles E., Wilkerson P.M., Ribas R., Anderson H., Mackay A., Ghazoui Z., Rani A., Osin P., Nerurkar A., Renshaw L., Larionov A., Miller W.R., Dixon J.M., Reis-Filho J.S., Dunbier A.K., Martin L.A, & Dowsett M. (2015). Integrative analyses identify modulators of response to neoadjuvant aromatase inhibitors in patients with early breast cancer. Breast Cancer Research : BCR, 17(1), 35.
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2

Authentication and Mycoplasma Testing of Cell Lines

Cited 1 time
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Cell lines were authenticated (most recent date listed [ ]) by the University of Arizona Genetics Core and mycoplasma tested (Lonza #LT07–418). Lab stocks were made following authentication and used for this study. MCF-7 (ATCC; DMEM+10% FBS [06/29/16]), T47D (ATCC; RPMI+10% FBS [02/08/17]), ZR75.1 (ATCC; RPMI+10% FBS [10/13/16]), MDA-MB-231 (ATCC; DMEM+10% FBS [10/13/16]), MDA-MB-134-VI (ATCC; 50/50 DMEM/L15+10% FBS [02/08/17]), SUM44PE (Asterand; DMEM/F12+2% CSS with 5ug/ml insulin, 1ug/ml hydrocortisone, 5mM ethanolamine, 5ug/ml transferrin, 10nM triodothyronime, and 50nM sodium selenite [02/08/17 – no reference profile exists in database]), and BCK426 (link) (MEM+5% FBS with 1nM insulin and 1× NEAA [10/13/16 – no reference profile exists in database) cells were cultured with indicated media conditions.
Nagle A.M., Levine K.M., Tasdemir N., Scott J.A., Burlbaugh K., Kehm J., Katz T.A., Boone D.N., Jacobsen B.M., Atkinson J.M., Oesterreich S, & Lee A.V. (2018). Loss of E-cadherin enhances IGF1-IGF1R pathway activation and sensitizes breast cancers to anti-IGF1R/InsR inhibitors. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(20), 5165-5177.
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3

VGLL4 Expression Vectors in Breast Cancer

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Human breast cell lines HCC1143, DU4475, MDA-MB-468, BT-474, MDA-MB-134-VI, AU565 and MDA-MB-453 and T47D, were purchased from ATCC (Manassas, VA). CAL-51 and CAL-120 cell lines were a kind gift from Dr. Toru Ouchi (Roswell Park Cancer Institute, NY). Cells were grown in DMEM medium (Corning Cellgro, NY), supplemented with 20% fetal bovine serum (FBS), penicillin (100 units/ml), streptomycin (100 µg/ml) under the 5% CO2 culture condition at 37 °C. VGLL4-WT was PCR amplified and inserted into the pBABE retroviral expression vector. VGLL4-ΔTUD1, ΔTUD2, and ΔTUD1&2 expression vectors were kindly provided by Dr. Ji4 (link).
Zhang Y., Shen H., Withers H.G., Yang N., Denson K.E., Mussell A.L., Truskinovsky A., Fan Q., Gelman I.H., Frangou C, & Zhang J. (2017). VGLL4 Selectively Represses YAP-Dependent Gene Induction and Tumorigenic Phenotypes in Breast Cancer. Scientific Reports, 7, 6190.
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4

Breast Cancer Cell Line Maintenance

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MCF7, T47D and MDA-MB-134-VI (MDAMB134) cells were purchased from ATCC; SUM44PE (SUM44) cells were a gift by Dr. Stephen Ethier. All the cells were authenticated by short tandem repeat (STR) profiling at Bio-Synthesis (USA) and regularly tested for mycoplasma contamination by Mycoalert detection Kit (Lonza). The MCF7 cells were maintained in DMEM and T47D cells in RPMI supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 1% penicillin/streptomycin (P/S). MDAMB134 cells were maintained in L-15 with 20%FBS and 1% P/S. SUM44 were maintained in Ham’s F-12 supplemented with insulin (5ug/mL), hydrocortisone (1ug/mL), fungizone (2.5ug/mL), transferrin (5ug/mL), T3 (6.6ng/mL), ethanolamin (5mM), NaSe (8.7ng/mL) (all from Sigma-Aldrich, St. Louis, MO), gentamicin (25ug/mL), HEPES (10mM), BSA (0.1%) (all from Thermo Fisher Scientific). For hormone deprived (HD) conditions, cells were kept in phenol-red free medium supplemented with 10% heat-inactivated charcoal-stripped (CS)-FBS (with the exception of SUM44) (20% for MDAMB134) and 1% P/S. MCF7, T47D and SUM44 were incubated at 37°C in 5% CO2, MDAMB134 were incubated at 37°C without CO2.
Nardone A., Qiu X., Spisak S., Nagy Z., Feiglin A., Feit A., Cohen Feit G., Xie Y., Font-Tello A., Guarducci C., Hermida-Prado F., Syamala S., Lim K., Munoz Gomez M., Pun M., Cornwell M., Liu W., Ors A., Mohammed H., Cejas P., Brock J.B., Freedman M.L., Winer E.P., Fu X., Schiff R., Long H.W., Metzger Filho O, & Jeselsohn R. (2022). A Distinct Chromatin State Drives Therapeutic Resistance in Invasive Lobular Breast Cancer. Cancer research, 82(20), 3673-3686.
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5

Comprehensive Cancer Cell Line Profiling

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A549, BT-20, BT-474, BT-549, CAMA-1, DMS-53, DU4475, HCC38, HCC70, HCC202, HCC1143, HCC1187, HCC1395, HCC1569, HCC1806, HCC1937, HCC1954, HCC2218, HCT-116, Hs578T, Jeko-1, MCF-7, MDA-MB-134-VI, MDA-MB-157, MDA-MB-175-VII, MDA-MB-231, MDA-MB-361, MDA-MB-415, MDA-MB-436, MDA-MB-453, MDA-MB-468, MiaPaCa2, SK-BR-3, NCI-H441, SK-MEL-28, T-47D, U2OS, and ZR-75-1 were obtained from the American Type Culture Collection (ATCC) and cultured according to vendor recommendations. EFM-19 were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ) and SNU-886 were from the Korean Cell Line Bank (KCLB).
Abemaciclib, palbociclib, ribociclib, PIM447, BYL719, LY2090314, everolimus, DYRK1Bi AZ cpd 33 [32 (link)], dinaciclib, GSK2334470, abemaciclib metabolites M2 and M20 [28 (link)], and additional CDK4/6i (see Figure 2C [33 ],) were synthesized by Lilly Research Laboratories. AZD1208 (S7104) and additional palbociclib (S1579, see Supplementary Figure 1A) were purchased from Selleck Chemicals.
Litchfield L.M., Boehnke K., Brahmachary M., Mur C., Bi C., Stephens J.R., Sauder J.M., Gutiérrez S.M., McNulty A.M., Ye X.S., Wu W., Lallena M.J., Gong X., Merzoug F.F., Jansen V.M, & Buchanan S.G. (2020). Combined inhibition of PIM and CDK4/6 suppresses both mTOR signaling and Rb phosphorylation and potentiates PI3K inhibition in cancer cells. Oncotarget, 11(17), 1478-1492.
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6

Cell Line Authentication and Maintenance

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MCF7, T47D, MDA-MB-134-VI, SUM44-PE, MCF10A and HEK293 were all purchased from American Type Culture Collection (ATCC) and identity authenticated by DNA fingerprinting (University of Arizona). Cells were routinely tested for mycoplasma and were negative at all times. MCF7 with ESR1-Y537S were generated previously 50 . BCK4 was a gift from Brita Jacobson (University of Colorado). Cells were maintained in media described in Supplementary Table 1.
Chen F., Ding K., Priedigkeit N., Elangovan A., Levine K.M., Carleton N., Savariau L., Atkinson J.M., Oesterreich S., & Lee A.V. (2020). Single cell transcriptomic heterogeneity in invasive ductal and lobular breast cancer cells.
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