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Mircury lna mirna pcr assay primer mix

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The MiRCURY LNA miRNA PCR assay primer mix is a laboratory equipment product designed for the detection and quantification of microRNA (miRNA) expression levels. The primer mix contains locked nucleic acid (LNA) technology, which enhances the sensitivity and specificity of the PCR assay.

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Lab products found in correlation

Mircury lna rt kit, by Qiagen (3 mentions) Seramir exosome rna amplification kit, by System Biosciences (3 mentions) Mircury lna mirna sybr green pcr kit, by Qiagen (3 mentions) Rt2 sybr green qpcr mastermix, by Qiagen (1 mentions)

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LNA primers (18 citations) PCR primers (10 citations) MiRNA primers (9 citations) Primer Mix (7 citations) LNA PCR primers (6 citations) MiRNA LNA primers (2 citations) LNA miRCuRY (2 citations) PCR primer mix (2 citations)

3 protocols using mircury lna mirna pcr assay primer mix

1

Profiling Brain-specific miRNAs in hiPSC-NSC-EVs

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Among the most abundant 40 miRNAs from RNA-Seq data, eight miRNAs that are known to contribute to various aspects of brain function were chosen and validated through quantitative real-time PCR. The total RNA was isolated from two biological replicates of hiPSC-NSC-EVs (~25 × 109 each) using the SeraMir Exosome RNA amplification kit (System biosciences). miRCURY LNA RT Kit (Qiagen, Germantown, MD, USA) was employed for converting 5 ng/μl of total RNA into cDNA. miRCURY LNA miRNA SYBR Green PCR kit (Qiagen) and miRCURY LNA miRNA PCR assay primer mix (Qiagen) were used to measure the comparative expression of 8 different miRNAs (miRNAs- 320a, 103a-3p, 21–5p, 26a-5p, 320b, 30a-3p, 181a-5p, 191–5p).
Upadhya R., Madhu L.N., Attaluri S., Gitaí D.L., Pinson M.R., Kodali M., Shetty G., Zanirati G., Kumar S., Shuai B., Weintraub S.T, & Shetty A.K. (2020). Extracellular vesicles from human iPSC-derived neural stem cells: miRNA and protein signatures, and anti-inflammatory and neurogenic properties. Journal of Extracellular Vesicles, 9(1), 1809064.
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2

Validating PTX3 and miR-21-5p Expression in hNSCs

Cited 2 times
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The reduced expression of PTX3 was first validated in control and transfected hNSCs through measurement PTX3 mRNA via qPCR using a specific primer (Qiagen, Germantown, MD, United States) and RT2 SYBR Green qPCR Mastermix (Qiagen). Then, PTX3 protein was measured from hNSC-lysates and in EVs isolated from control and transfected hNSCs using sandwich ELISA (Aviscera Biosciences, Sunnyvale, CA, United States). Our previous reports describe the detailed qPCR and ELISA methods employed (Upadhya et al., 2020b (link); Madhu et al., 2021 (link)). Diminished expression of miR-21-5p was validated by measuring miR-21-5p in EVs generated from control and transfected hNSCs, via qPCR. For this, the total RNA from hNSC-EVs was first isolated using the SeraMir Exosome RNA amplification kit (System Biosciences), following which miRCURY LNA RT Kit (Qiagen) was employed for converting 5 ng/μl of total RNA into cDNA. miRCURY LNA miRNA SYBR Green PCR kit (Qiagen) and miRCURY LNA miRNA PCR assay primer mix (Qiagen) were employed to measure miR-21–5p level in hNSC-EVs.
Upadhya R., Madhu L.N., Rao S, & Shetty A.K. (2022). Proficiency of Extracellular Vesicles From hiPSC-Derived Neural Stem Cells in Modulating Proinflammatory Human Microglia: Role of Pentraxin-3 and miRNA-21-5p. Frontiers in Molecular Neuroscience, 15, 845542.
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3

Validation of Microglia-Modulating miRs in hMSC-EVs

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Among the top 20 enriched miRs from RNA-Seq data, eight miRs that are known to modulate activated microglia and/or NLRP3 inflammasomes were chosen and validated through quantitative real-time PCR. The total RNA was isolated from two biological replicates of hMSC-EVs (10 × 109 each) using the SeraMir Exosome RNA amplification kit (System Biosciences, Palo Alto, CA, USA). miRCURY LNA RT Kit (Qiagen, Germantown, MD, USA) was employed for converting 5 ng/μl of total RNA into cDNA. miRCURY LNA miRNA SYBR Green PCR kit (Qiagen) and miRCURY LNA miRNA PCR assay primer mix (Qiagen) were used to measure the comparative expression of 8 different miRs.
Kodali M., Madhu L.N., Reger R.L., Milutinovic B., Upadhya R., Gonzalez J.J., Attaluri S., Shuai B., Gitai D.L., Rao S., Choi J.M., Jung S.Y, & Shetty A.K. (2022). Intranasally Administered Human MSC-derived Extracellular Vesicles Inhibit NLRP3-p38/MAPK Signaling after TBI and Prevent Chronic Brain Dysfunction. Brain, behavior, and immunity, 108, 118-134.
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