Alanine transaminase alt

Manufactured by Merck Group

Sourced in United States

Alanine transaminase (ALT) is an enzyme that catalyzes the transfer of an amino group from alanine to α-ketoglutarate, producing pyruvate and glutamate. It is commonly used as a biomarker to assess liver function and detect liver injury.

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Lab products found in correlation

Creatinine, by Merck Group (2 mentions) Aspartate transaminase ast, by Merck Group (2 mentions) Alkaline phosphates alp, by Merck Group (2 mentions) 1 1 diphenyl 2 picrylhydrazyl dpph, by Merck Group (2 mentions) L ascorbic acid, by Merck Group (2 mentions) Glutathione peroxidase gpx, by Cayman Chemical (1 mentions) Aspartate aminotransferase ast, by Merck Group (1 mentions) Malondialdehyde mda, by Abcam (1 mentions) Tnf α, by R&D Systems (1 mentions) Advia 120, by Bayer (1 mentions) Alpha naphthylisothiocyanate anit, by Merck Group (1 mentions) Mouse total ba assay kit, by Crystal Chem (1 mentions) Rutin, by Merck Group (1 mentions) Quercetin, by Merck Group (1 mentions) Sodium phosphate buffer, by Merck Group (1 mentions) Linoleic acid, by Merck Group (1 mentions)

Close spelling variants of this product

Alanine transaminase Alanine

4 protocols using alanine transaminase alt

Immune and Biochemical Markers Evaluation

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Blood samples were collected into non-heparinized and K₃-EDTA vacuum
tubes (Becton Dickinson Vacutainer Systems) to determine numbers of immune
cells, including leukocytes, lymphocytes, and monocytes, after 3 and 4 weeks.
Immune cell counts were determined using an automatic blood analyzer (ADVIA120;
Bayer, Leverkusen, Germany). ELISA was used to quantify serum concentrations of
tumor necrosis factor (TNF)-α (R&D Systems,
Minneapolis, MN, USA), alanine transaminase (ALT; Sigma-Aldrich, St. Louis, MO,
USA), aspartate aminotransferase (AST; Sigma-Aldrich, St. Louis, MO, USA),
superoxide dismutase (SOD; Cohesion Biosciences, USA), malondialdehyde (MDA;
Abcam, Cambridge, UK), and glutathione peroxidase (GPx; Cayman Chemical, Ann
Arbor, MI, USA).

Kang K.S., Shin S, & Lee S.I. (2020). N-acetylcysteine modulates cyclophosphamide-induced immunosuppression, liver injury, and oxidative stress in miniature pigs. Journal of Animal Science and Technology, 62(3), 348-355.

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Recombinant FGF19 and FGF1 Protein Production

Cited 1 time

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Full-length wide type recombinant human FGF19 (FGF19^WT) and the FGF1 variant (FGF1^ΔHBS) carrying triple mutations (Lys127Asp, Lys128Gln, and Lys133Val) were expressed in E.coli (BL21) and purified as described in the previous studies (Goetz et al., 2007 (link); Huang et al., 2017 (link)). Mouse Total BA Assay Kit was purchased from Crystal Chem INC. Alpha-naphthylisothiocyanate (ANIT) was purchased from Sigma Aldrich (St. Louis, MO, USA). Alanine transaminase (ALT) and aspartate transaminase (AST) Assay Kits were purchased from Sigma Aldrich (St. Louis, MO, USA) and performed in accordance with the protocols provided with manufacturers. All other reagents were of analytical grade.

Lin H., Zhou C., Hou Y., Li Q., Qiao G., Wang Y., Huang Z, & Niu J. (2019). Paracrine Fibroblast Growth Factor 1 Functions as Potent Therapeutic Agent for Intrahepatic Cholestasis by Downregulating Synthesis of Bile Acid. Frontiers in Pharmacology, 10, 1515.

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Antioxidant and Hepatoprotective Rutin Protocol

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Rutin (≥ 98％) was obtained from Sigma (Sigma-Aldrich GmbH, Sternheim, Germany) , and kept at 110℃ under 1.3 kPa for 12 h to remove bound water. Quercetin, BHA (butyl-hydroxyanisole) , BHT (butylhydroxytoluene) , TBHQ (tert.butylhydro quinine) , L-ascorbic acid, and linpalmitic acid were purchased from Merck. 1,1-Diphenyl-2-picryl-hydrazyl (DPPH • ) , sodium phosphate buffer, tween 20 and absolute ethanol, Alanine transaminase (ALT) , Aspartate transaminase (AST) , Alkaline phosphates (ALP) , creatinine, and urea kites were purchased from Sigma (Sigma-Aldrich GmbH, Sternheim, Germany) . All other used chemicals were of analytical grade and were obtained from Merck and Sigma (Sigma-Aldrich GmbH, Sternheim, Germany) . Animals were obtained from the animal house -National research centre.

Soliman H.M. (2023). Preparation of a Regioselective Quercetin-3-palmitate and Its Using for Boosting Cooking Oil Stability. Journal of oleo science, 72(2).

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Antioxidant and Hepatoprotective Potential

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Commercially tannic acid was purchased from El-Gomhoria Trade Pharmaceuticals & Chemicals Company, Cairo, Egypt. BHA (butyl-hydroxyanisole) , BHT (butylhydroxytoluene) , BHQ (tert.butylhydro quinine) , L-ascorbic acid and linoleic acid were purchased from Merck. 1,1-Diphenyl-2-picryl-hydrazyl (DPPH ・ ) , sodium phosphate buffer, tween 20 and absolute ethanol, alanine transaminase (ALT) , aspartate transaminase (AST) , alkaline phosphates (ALP) , creatinine and urea kites were purchased from sigma (sigma-aldrich GmbH, sternheim, germany) . All other chemicals used were of analytical grade and were obtained from merck and sigma (sigma-aldrich GmbH, sternheim, germany) . Animals were obtained from animal house -National research centre.

Soliman H.M., Arafat S.M., Basuny A.M, & Shattory Y.E. (2017). Synthesis and Application of a New Amphiphilic Antioxidant. Journal of oleo science, 66(11).

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