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Membrane impermeable halotag ligand mil

Manufactured by Promega
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The Membrane-impermeable HaloTag Ligand (MIL) is a lab equipment product that functions as a cell-impermeant ligand for the HaloTag protein. The MIL can be used to label and detect HaloTag-fusion proteins localized to the extracellular surface of cells.

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Lab products found in correlation

Map1lc3b, by Novus Biologicals (2 mentions) Vps28, by Santa Cruz Biotechnology (2 mentions) Membrane permeable halotag ligand mpl, by Promega (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Xf plasmamembrane permeabilizer xf pmp, by Agilent Technologies (2 mentions) Nucleofector kit 5, by Lonza (2 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (2 mentions) Normal goat serum (ngs), by Merck Group (2 mentions) β actin, by Merck Group (2 mentions) 03 gp62 c, by Abcam (2 mentions) Tsg101, by Abcam (2 mentions) Bafilomycin a1, by LC Laboratories (2 mentions)

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HaloTag ligand (16 citations)

2 protocols using membrane impermeable halotag ligand mil

1

Comprehensive Antibody Validation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for immunoblotting (IB) and immunofluorescence (IF): β-ACTIN (IB, Sigma-Aldrich, A5441, 1:10,000); GFP (IB, Cell Signaling, 2956, 1:1,000); HA (IB, IF, BioLegend, 901513, 1:2,000 (IB), 1:1,000 (IF)); MAP1LC3B (IB, Novus, NB100–2220, 1:3,000; IF, Cell Signaling, 3868, 1:200); p62 (IB; American Research Products, 03-GP62-C, 1:4,000); TSG101 (IB, Abcam, ab83, 1:1,000); VPS28 (IB, Santa Cruz Biotechnology, sc-166537, 1:100). ON-TARGETplus SMART Pool Non-targeting (D-001810–10) and CHMP2A (L-020247–01) siRNAs were obatined from GE Healthcare Dharmacon. pCDH1-CMV-HA-VPS28(WT)-SV40-hygro and pCDH1-CMV-HA-VPS28(TM[K54D, K58D, D59A])-SV40-hygro were generated using Gibson Assembly. All other reagents were obtained from the following sources: Bafilomycin A1 (LC Laboratories, B-1080); Hoechst 33342 (Invitrogen, NucBlue, R37605); Membrane-impermeable HaloTag Ligand (MIL) (Promega, Alexa Fluor 488-conjugated, G1001); Membrane-permeable HaloTag Ligand (MPL) (Promega, tetramethylrhodamine-conjugated, G8251); normal goat serum (Sigma-Aldrich, G9023); Nucleofector Kit V (Lonza, VCA-1003); paraformaldehyde (Electron Microscopy Sciences, 15710); XF Plasma Membrane Permeabilizer (XF-PMP) (Seahorse Bioscience, 102504–100).
Flower T.G., Takahashi Y., Hudait A., Rose K., Tjahjono N., Pak A.J., Yokom A.L., Liang X., Wang H.G., Bouamr F., Voth G.A, & Hurley J.H. (2020). A helical assembly of human ESCRT-I scaffolds reverse-topology membrane scission. Nature structural & molecular biology, 27(6), 570-580.
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2

Comprehensive Antibody Validation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for immunoblotting (IB) and immunofluorescence (IF): β-ACTIN (IB, Sigma-Aldrich, A5441, 1:10,000); GFP (IB, Cell Signaling, 2956, 1:1,000); HA (IB, IF, BioLegend, 901513, 1:2,000 (IB), 1:1,000 (IF)); MAP1LC3B (IB, Novus, NB100–2220, 1:3,000; IF, Cell Signaling, 3868, 1:200); p62 (IB; American Research Products, 03-GP62-C, 1:4,000); TSG101 (IB, Abcam, ab83, 1:1,000); VPS28 (IB, Santa Cruz Biotechnology, sc-166537, 1:100). ON-TARGETplus SMART Pool Non-targeting (D-001810–10) and CHMP2A (L-020247–01) siRNAs were obatined from GE Healthcare Dharmacon. pCDH1-CMV-HA-VPS28(WT)-SV40-hygro and pCDH1-CMV-HA-VPS28(TM[K54D, K58D, D59A])-SV40-hygro were generated using Gibson Assembly. All other reagents were obtained from the following sources: Bafilomycin A1 (LC Laboratories, B-1080); Hoechst 33342 (Invitrogen, NucBlue, R37605); Membrane-impermeable HaloTag Ligand (MIL) (Promega, Alexa Fluor 488-conjugated, G1001); Membrane-permeable HaloTag Ligand (MPL) (Promega, tetramethylrhodamine-conjugated, G8251); normal goat serum (Sigma-Aldrich, G9023); Nucleofector Kit V (Lonza, VCA-1003); paraformaldehyde (Electron Microscopy Sciences, 15710); XF Plasma Membrane Permeabilizer (XF-PMP) (Seahorse Bioscience, 102504–100).
Flower T.G., Takahashi Y., Hudait A., Rose K., Tjahjono N., Pak A.J., Yokom A.L., Liang X., Wang H.G., Bouamr F., Voth G.A, & Hurley J.H. (2020). A helical assembly of human ESCRT-I scaffolds reverse-topology membrane scission. Nature structural & molecular biology, 27(6), 570-580.
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