Elf1 cells, a naïve human ESC line isolated from an 8-cell human embryo, were procured from Wicell (Madison, WI, USA) and maintained according to a previously published protocol [22 (link)]. Briefly, Elf1 cells were grown adherent to mouse embryonic feeder (MEF) coated 6-well plates and incubated in a medium containing KnockOut/F12 DMEM with GlutaMax (Life Technologies, Carlsbad, CA, USA) with 20% KnockOut serum replacement (Life Technologies), 1 mM sodium pyruvate (Life Technologies), 0.1 mM 2-Mercaptoehtanol (Life Technologies), 1% non-essential amino acids (Life Technologies), and 0.2% penicillin-streptomycin solution (Life Technologies), and supplemented with 12 ng/mL FGF2 (Prospec, Ness Ziona, Israel), 1.5 μM CHIR99021 (Cayman Chemical, Ann Arbor, MI, USA), 0.4 μM PD03296501 (Caymen Chemical) and 0.01 μg/mL human LIF (Prospec). Prior to encapsulation in 3-D scaffolds, cells were transitioned to feeder-free culture in Matrigel-coated 6-well plates (Fisher Scientific, Pittsburgh, PA, USA).
Human lif
Manufactured by ProSpec
Sourced in United States
Human LIF is a recombinant protein that functions as a growth factor. It plays a role in the maintenance of embryonic stem cells and the self-renewal of undifferentiated cells.
Automatically generated - may contain errors
Lab products found in correlation
Sodium pyruvate, by Thermo Fisher Scientific (2 mentions)
Knockout serum replacement (ksr), by Thermo Fisher Scientific (2 mentions)
Knockout f12 dmem with glutamax, by Thermo Fisher Scientific (2 mentions)
2 mercaptoehtanol, by Thermo Fisher Scientific (2 mentions)
Matrigel coated 6 well plates, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (2 mentions)
Chir99021, by Cayman Chemical (2 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions)
Fibroblast growth factor 2 (fgf2), by ProSpec (2 mentions)
2 protocols using human lif
1
Naive Human ESC Line Maintenance
2
Cultivating Naïve Human ESCs on MEFs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Elf1 cells, a naïve human ESC line isolated from an 8-cell human embryo, were procured from Wicell (Madison, WI), and maintained according to a previously published protocol [22] . Briefly, Elf1 cells were grown adherent to mouse embryonic feeder (MEF) coated 6-well plates, and incubated in medium containing KnockOut/F12 DMEM with GlutaMax (Life Technologies, Carlsbad, CA) with 20% KnockOut serum replacement (Life Technologies), 1mM sodium pyruvate (Life Technologies), 0.1mM 2-Mercaptoehtanol (Life Technologies), 1% non-essential amino acids (Life Technologies), and 0.2% penicillin-streptomycin solution (Life Technologies), and supplemented with 12ng/ml FGF2 (Prospec, Ness Ziona, Israel), 1.5µM CHIR99021 (Cayman Chemical, Ann Arbor, MI), 0.4µM PD03296501 (Caymen Chemical) and 0.01µg/ml human LIF (Prospec). Prior to encapsulation in 3-D scaffolds, cells were transitioned to feeder-free culture in Matrigel-coated 6-well plates (Fisher Scientific, Pittsburgh, PA).
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