Cell lysates were prepared by washing cells in cold PBS followed by addition of SDS-PAGE sample buffer containing Protease Inhibitor Cocktail (SIGMA Cat#P2714–1BTL). Cells were transferred to a 1.5ml tube, sonicated, and heated at 95°C for 5 min. Proteins were separated by electrophoresis in NuPAGE 10% Bis-Tris gels and transferred to PVDF membranes (Milipore- Immobilon, FL, Cat #IPFL00010- pore size: 0.45μm) by electroblotting. Membranes were blocked in PBS+ 5% milk powder and incubated with primary antibodies and anti-SEPT9 rabbit antibody (Proteintech Cat#10769–1-AP) and α-TUBULIN mouse antibody (Sigma Cat #T9026) overnight at 4°C. The other antibodies used were MMP3 rabbit antibody (Arigo Biolaboratories Cat#ARG55262), CD9 mouse antibody (Proteintech Cat#60232–1-Ig), or Actin mouse antibody (Proteintech Cat#66009–1-Ig). After three washes in PBS-T, membranes were incubated with secondary antibodies (Mouse 680 and Rabbit 800 [LI-COR Biosciences]) for one hour at room temperature, washed three times in PBS-T, and scanned using a high-sensitivity Odyssey Infrared Imaging System (Li-COR Biosciences). α-TUBULIN was used as a loading control. ImageJ was used for quantification of bands intensities.
Mmp3 rabbit antibody
Manufactured by Arigo Biolaboratories
The MMP3 rabbit antibody is a molecular biology tool used to detect and quantify the presence of the matrix metalloproteinase 3 (MMP3) protein in biological samples. MMP3 is an enzyme involved in the breakdown of extracellular matrix components and plays a role in various physiological and pathological processes. This antibody can be utilized in techniques such as Western blotting, immunohistochemistry, and ELISA to study the expression and localization of MMP3 in different cell and tissue types.
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2 protocols using mmp3 rabbit antibody
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Western Blot Analysis of Cell Lysates
2
Western Blot Analysis of Cell Lysates
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Cell lysates were prepared by washing cells in cold PBS followed by addition of SDS-PAGE sample buffer containing Protease Inhibitor Cocktail (SIGMA Cat#P2714–1BTL). Cells were transferred to a 1.5ml tube, sonicated, and heated at 95°C for 5 min. Proteins were separated by electrophoresis in NuPAGE 10% Bis-Tris gels and transferred to PVDF membranes (Milipore- Immobilon, FL, Cat #IPFL00010- pore size: 0.45μm) by electroblotting. Membranes were blocked in PBS+ 5% milk powder and incubated with primary antibodies and anti-SEPT9 rabbit antibody (Proteintech Cat#10769–1-AP) and α-TUBULIN mouse antibody (Sigma Cat #T9026) overnight at 4°C. The other antibodies used were MMP3 rabbit antibody (Arigo Biolaboratories Cat#ARG55262), CD9 mouse antibody (Proteintech Cat#60232–1-Ig), or Actin mouse antibody (Proteintech Cat#66009–1-Ig). After three washes in PBS-T, membranes were incubated with secondary antibodies (Mouse 680 and Rabbit 800 [LI-COR Biosciences]) for one hour at room temperature, washed three times in PBS-T, and scanned using a high-sensitivity Odyssey Infrared Imaging System (Li-COR Biosciences). α-TUBULIN was used as a loading control. ImageJ was used for quantification of bands intensities.
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