All naive RAG1−/− mice were i.v. reconstituted with 5×106 CD4+ T cells. CD4+ T cells are isolated by first B220+ depletion, followed by anti-mouse L3T4 magnetic bead selection (Miltenyi Biotec) from the spleens of WT mice. Indicated mice were i.v. reconstituted with 10 × 106 naive B2 cells. B2 cells are isolated by depletion using either anti-mouse CD43 Miltenyi microbeads (Miltenyi Biotec) or biotinylated anti-mouse CD43 (AB_493384) followed by anti-biotin microbeads (Miltenyi Biotec) from the spleens of WT mice. Indicated mice were i.p. reconstituted with 2–4 × 105 B1 cells. B1 cells are isolated from the peritoneal and pleural cavities of WT mice (Yenson and Baumgarth, 2014 (link)). Briefly, cells were Fc blocked on ice for 10 min (2.4G2) (Unkeless, 1979 (link)) followed by the following biotinylated antibodies for 30 min on ice: anti-mouse CD23 (B3B4) (AB_312829), anti- mouse CD49b (DX5) (AB_313035), anti-mouse F4/80 (BM8) (AB_893499), anti-mouse CD90.2 (30-H12) (AB_313175), and anti-mouse GR-1 (RB6-8C5) (AB_313368). After washing, anti-biotin microbeads (Miltenyi Biotec) were added, and magnetic bead depletion was performed for B1 enrichment. Mice were not used in experiments until 1 week post reconstitution.
Anti mouse cd43
Manufactured by Miltenyi Biotec
Anti-mouse CD43 is a laboratory equipment product used for the detection and analysis of the CD43 protein in mouse samples. CD43 is a cell surface glycoprotein that is expressed on various hematopoietic cells, including T cells, B cells, and monocytes. This product can be used for applications such as flow cytometry and cell sorting.
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2 protocols using anti mouse cd43
1
Adoptive Transfer of T and B Cells in RAG1-/- Mice
2
Adoptive Transfer of T and B Cells in RAG1-/- Mice
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All naive RAG1−/− mice were i.v. reconstituted with 5×106 CD4+ T cells. CD4+ T cells are isolated by first B220+ depletion, followed by anti-mouse L3T4 magnetic bead selection (Miltenyi Biotec) from the spleens of WT mice. Indicated mice were i.v. reconstituted with 10 × 106 naive B2 cells. B2 cells are isolated by depletion using either anti-mouse CD43 Miltenyi microbeads (Miltenyi Biotec) or biotinylated anti-mouse CD43 (AB_493384) followed by anti-biotin microbeads (Miltenyi Biotec) from the spleens of WT mice. Indicated mice were i.p. reconstituted with 2–4 × 105 B1 cells. B1 cells are isolated from the peritoneal and pleural cavities of WT mice (Yenson and Baumgarth, 2014 (link)). Briefly, cells were Fc blocked on ice for 10 min (2.4G2) (Unkeless, 1979 (link)) followed by the following biotinylated antibodies for 30 min on ice: anti-mouse CD23 (B3B4) (AB_312829), anti- mouse CD49b (DX5) (AB_313035), anti-mouse F4/80 (BM8) (AB_893499), anti-mouse CD90.2 (30-H12) (AB_313175), and anti-mouse GR-1 (RB6-8C5) (AB_313368). After washing, anti-biotin microbeads (Miltenyi Biotec) were added, and magnetic bead depletion was performed for B1 enrichment. Mice were not used in experiments until 1 week post reconstitution.
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