Human neutrophil elastase platinumelisa kit

Peripheral blood samples were collected in sodium citrate tubes (Becton Dickinson, San Jose, CA, USA). After centrifuging the whole blood samples for 15 min at 1550 × g, the plasma was aliquoted and stored at -70°C, with subsequent thawing for further analyses.
The histone-DNA complex levels were measured using a cell death detection ELISA kit (Roche Diagnostics, Indiana, USA). Cell-free DNA levels were measured using the Quant-iT PicoGreen dsDNA reagent (Molecular Probes, Eugene, Oregon, USA) and Fluoroskan Ascent microplate fluorometer (Thermo Fisher Scientific Inc., Waltham, Massachusetts, USA). Neutrophil elastase levels were measured using a human neutrophil elastase platinum ELISA kit (eBioscience, Vienna, Austria). Prekallikrein levels were determined using an ELISA kit (Cloud-Clone, Houston, Texas, USA). Initial serum CA-125 levels were evaluated using an immunoradiometric assay kit (Institute of Isotopes, Budapest, Hungary).