Peo1 10032308

Two human ovarian cancer cell lines (SKOV3 and PEO1) and a normal human fibroblast MRC-5 cell line were used. The SKOV3 (HTB-77) and MRC-5 (CCL-171) cells were purchased from the American Type Culture Collection (ATCC). The PEO1 (10032308) cells were obtained from the European Collection of Authenticated Cell Cultures (ECACC).
The SKOV3 cells were cultured in McCoy’s 5A medium, the PEO1 cells in RPMI + GlutaMAX medium, and the MRC-5 cells in DMEM + GlutaMAX. All the media contained 10% heat-inactivated FBS and 1% v/v penicillin/streptomycin solution. The cells were cultured at 37 °C under 5% carbon dioxide and 95% humidity. They were passaged after reaching about 85% confluence. The Trypan Blue exclusion test was used for the evaluation of cell viability. A Thoma hemocytometer (Superior Marienfeld, Lauda-Königshofen, Germany) was used for cell counting.

The human OC cell line (SKOV3 (HTB-77)) and human lung normal fibroblast cell line (MRC-5 (CCL-171)) were obtained from the American Type Culture Collection (ATCC). We also used the second OC cell line derived from humans (PEO1 (10032308)) purchased in the European Collection of Authenticated Cell Cultures (ECACC). The SKOV3 cell line was a hypodiploid OC derived from a 64-year-old Caucasian female with an ovarian serous cyst adenocarcinoma. The number of chromosomes was 43, occurring in over 60% of the cells. These adenocarcinoma cells were positive for many antigens generally used to identify epithelial cancer—for example: EMA (epithelial membrane antigen), VIM (vimentin) and cytokeratin alike.
The second OC cell line used in the experiments was PEO1 derived from a malignant effusion from the peritoneal ascites of a patient with a poorly differentiated serous adenocarcinoma after treatment with chlorambucil, 5-fluorouracil and cisplatin. This cell line was positive for hormone receptors like the estrogen receptor.
Normal human fibroblasts were used as control cells. The MRC-5 fibroblast line was derived from normal lung tissue of a 14-week-old male fetus. This is a normal diploid human cell line with 46 XY karyotypes.