Cell proliferation and colony assays were conducted as previously reported [19 ]. For the proliferation assay, cells were seeded into 96-well plates with a density of 1000 cells/well. CCK-8 reagents (MCE, Monmouth Junction, NJ, USA) were then added into cells at the indicated time and incubation about 2 h before the OD measurement. Cell proliferation was also assessed using a Cell-Light EdU DNA cell proliferation kit (RiboBio, Guangzhou, China), following the manufacturer’s instructions. For the colony assay, 1500 control cells and the treated cells were seeded into 12-well plates and cultured for one week. Colonies were fixed with methanol and stained with 0.1% crystal violet.
Cell light edu dna cell proliferation kit
Manufactured by RiboBio
Sourced in China, Japan
The Cell-Light EdU DNA Cell Proliferation Kit is a laboratory equipment product designed to detect and quantify cell proliferation. The kit utilizes the incorporation of the thymidine analog EdU (5-ethynyl-2'-deoxyuridine) into newly synthesized DNA, providing a direct measurement of DNA replication during the S phase of the cell cycle. The kit includes necessary reagents and protocols to enable researchers to visualize and analyze cell proliferation in their samples.
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Lab products found in correlation
Fsx100 microscope, by Olympus (23 mentions)
Dmr fluorescence microscope, by Leica (18 mentions)
Fluorescence microscope, by Olympus (11 mentions)
Fluorescence microscope, by Leica (8 mentions)
Inverted fluorescence microscope, by Zeiss (7 mentions)
Cell counting kit 8 (cck8), by Dojindo Laboratories (7 mentions)
Cck 8 solution, by RiboBio (6 mentions)
Cck 8, by Dojindo Laboratories (5 mentions)
Pi rnase staining buffer, by BD (5 mentions)
Fluorescent microscope, by Olympus (5 mentions)
Dm5000b, by Leica (5 mentions)
Facscanto 2, by BD (5 mentions)
Accuri c6, by BD (5 mentions)
Cck 8 kit, by Dojindo Laboratories (4 mentions)
Microplate reader, by Molecular Devices (4 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (4 mentions)
Cck 8, by Beyotime (4 mentions)
Dmi8 fluorescence microscope, by Leica (4 mentions)
Paraformaldehyde (pfa), by Solarbio (4 mentions)
5 ethynyl 2 deoxyuridine (edu), by RiboBio (4 mentions)
303 protocols using cell light edu dna cell proliferation kit
1
Cell Proliferation and Colony Assays
2
Cell Proliferation Assessed by EdU Assay
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Cell proliferation was tested by means of EdU assay using Cell-Light EdU DNA Cell Proliferation Kit (RiboBio, China) according to the manufacturer’s protocol [20 (link)]. Images were photographed and counted in three randomly selected fields under an FSX100 microscope (Olympus, Japan).
3
Cell Proliferation Assay with EdU Staining
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The cell proliferation was tested by EdU assay using Cell-Light EdU DNA Cell Proliferation Kit (RiboBio, Guangzhou, China). After incubation at 37°C and 5% CO2 for 48h, transfected cells were added with 50mM EdU and incubated for another 2h. Cells were then fixed with 4% paraformaldehyde and stained with Apollo Dye Solution for proliferating cells. Nucleic acids in all cells were stained with DAPI. The cell proliferation rate was calculated using ImageJ software (version 1.8.0; National Institutes of Health, Sacaton, AZ, USA). Images were taken using a fluorescence microscope.
4
EdU Proliferation Assay Protocol
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The EdU assay was performed using a Cell-Light EdU DNA Cell Proliferation Kit (RiboBio, Shanghai, PR, China) according to the manufacturer’s instructions.
5
Cell Proliferation Assay using EdU
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The cells in each group were collected and plated in a 96-well plate (2 × 104 cells/well). The proliferation ability was detected using a Cell-Light™ EdU DNA Cell Proliferation Kit (Ribobio, Guangzhou, China), following the manufacturer’s instructions.
6
HCC Cell Proliferation and Colony Formation Analysis
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The proliferation of HCC cells was detected with Cell Counting Kit-8 (CCK-8, Bosterbio, Wuhan, China) and Cell-Light™ EdU DNA Cell Proliferation Kit (Ribobio, Guangzhou, China) in accordance with the manufacturer's protocols, respectively. For colony formation assays, HCC cells (2000/well) were seeded into 6-well plates and cultured for 10 days. The cells were washed 3 times with PBS and fixed with 4% paraformaldehyde for 20 min and followed by staining with crystal violet for 10 min. The stained cells were photographed with a microscope (Leica, Wetzlar, Germany).
7
Quantifying CCA Cell Proliferation
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EdU (5-ethynyl-2′-deoxyuridine) is a thymidine analog that can be used to label proliferating cells. We used the Cell-Light EdU DNA Cell Proliferation Kit (RiboBio, Shanghai, China) to detect the proliferation function of different CCA cells according to the manufacturer’s instructions. Cells were seeded in each well of 6-well plates, and the nucleic acids in all cells were stained with DAPI (4′,6-diamidino-2-phenylindole) dye. Images were acquired using a fluorescence microscope (Olympus FSX100).
8
Cell Proliferation Assay Using EdU
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The EdU assay was carried out with a Cell-Light EdU DNA Cell Proliferation Kit (RiboBio, Shanghai, PR, China). 1 × 104 cells were seeded in 96-well plate. After incubation with 50 mM EdU for 2 h, the cells were fixed in 4% paraformaldehyde and stained with Apollo Dye Solution. Hoechst-33,342 was used to stain the nucleic acid within the cells. Images were acquired with an Olympus FSX100 microscope (Olympus, Tokyo, Japan), and the percentage of EdU-positive cells was calculated.
9
MSC Proliferation and Viability Assay
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The proliferation activity and viability of MSCs compounding with or without fibrin glue were assayed using the Cell-Light™ EdU DNA Cell Proliferation Kit (Guangzhou Ribobio Co., Ltd, Guangzhou, China) and the Cell Counting Kit-8 (CCK-8, Dojindo Laboratorise, Tokyo, Japan) according to the manufacturer’s instruction and as described before
[22 (link)].
[22 (link)].
10
Cell Proliferation Quantification with EdU
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Cell‐Light EdU DNA cell proliferation kit (Ribo Bio) was used to determine the proliferation rate of A549R and H1299R cells according to the manufacturer's instructions. Briefly, cells were incubated with 50 μM EdU for 2 h, then fixed, permeabilized, and stained with EdU. The nuclei were stained with Hoechst 33342 at a concentration of 5 μg/ml for 30 min, and the cells were then inspected using a fluorescence microscope (Olympus). The viability index was calculated as follows: Viability index = experimental OD value/control OD value. In addition, 5‐ethynyl‐20‐deoxyuridine (EdU) was used to evaluate cell proliferation.
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