Materials. All components of conventional blank LNCs are FDA-approved for parenteral administration. Macrogol 15 hydroxystearate (Kolliphor® HS 15) was purchased from BASF (Germany). Phosphatidylcholine from soybean (Lipoïd S 100) was provided by Lipoïd GmbH (Germany) and caprylic/capric triglycerides (Labrafac® WL 1349) were supplied by Gattefosse (France). Ultra-pure water (UPW) was obtained from a Millipore filtration system. All other reagents or solvents used (acetonitrile, methanol, dimethyl sulfoxide DMSO) for chromatography were of analytical grade. Ferrocifens P722, 16 P849, 17 (link) and P769 35 (link) were synthesized as described previously. Esterase from porcine liver was purchased from Merck (SigmaAldrich, Germany). Starting materials for the synthesis of P998 were obtained commercially from Sigma-Aldrich or Alfa Aesar France. Thin layer chromatography was performed on silica gel 60 GF254. IR spectra were obtained on a FT/IR-4100 JASCO 180 spectrometer. 1 H and 13 (link) C NMR spectra were acquired on Bruker 300 or 400 MHz spectrometers. High-resolution mass spectra (HRMS) were acquired in the "Institut Parisien de Chimie Moléculaire (IPCM -UMR 8232)" at Sorbonne Université, Paris.
Kolliphor hs 15
Manufactured by BASF
Sourced in Germany, France, China, Japan
Kolliphor HS 15 is a non-ionic surfactant used in various laboratory applications. It is a polyethylene glycol-based compound that can be utilized as an emulsifier, solubilizer, or wetting agent in formulations. The core function of Kolliphor HS 15 is to facilitate the dispersion and solubilization of hydrophobic substances in aqueous media.
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Lab products found in correlation
Kolliphor el, by BASF (8 mentions)
Kolliphor rh40, by BASF (8 mentions)
Oleic acid, by Merck Group (8 mentions)
Kolliphor elp, by BASF (6 mentions)
Kollisolv mct 70, by BASF (6 mentions)
Kolliphor ps80, by BASF (5 mentions)
S75 3, by Lipoid (5 mentions)
Capryol 90, by Gattefosse (4 mentions)
Milli q system, by Merck Group (4 mentions)
Tween 20, by Merck Group (4 mentions)
Tween 80, by Merck Group (4 mentions)
Hplc grade acetonitrile, by Thermo Fisher Scientific (4 mentions)
Kolliphor p188, by BASF (3 mentions)
Kolliphor ps20, by BASF (3 mentions)
Labrafil m 1944 cs, by Gattefosse (3 mentions)
Milli q plus system, by Merck Group (3 mentions)
Peg 400, by Tianjin Zhiyuan (3 mentions)
Labrafac wl1349, by Gattefosse (2 mentions)
Kollisolv peg 400, by BASF (2 mentions)
Transcutol p, by Gattefosse (2 mentions)
67 protocols using kolliphor hs 15
1
Ferrocifens Synthesis and Characterization
2
Xenograft Tumor Growth in Nude Mice
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The animal experiments complied with the requirements of the Institutional Animal Care and Use Committee of Shanghai Jiao Tong University (No. 201801054). Four-week-old male nude mice (Lingchang BioTech, n = 6 per group) were injected subcutaneously 5 × 106 HGC27 cells in their front flank. Tumor size was measured every 3 days. The calculation formula of tumor volume is (length × width × height)/2. After 2 weeks, when the size of the engrafted tumor came to about 100 mm3, the mice were injected intraperitoneally either ARV-825 at 10 mg/kg or menstruum control (10% Kolliphor®HS15, BASF) every day. The mice were sacrificed when the tumors in the control group exceeded 1,000 mm3. The tumors were excised and then embedded in paraffin for immunohistochemistry.
3
Lipopolysaccharide-induced Inflammation Assay
4
Formulation and Characterization of PZQ-loaded Lipid Nanocarriers
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The following chemicals were used in the study: PZQ (high purity, gift of the Egyptian International Pharmaceuticals Industries Company (EIPICO), Cairo, Egypt); MFS (98-102%, Chem-Impex International, New York, USA); Labrafac ® lipophile WL 1349 (Gattefossé SA, Saint-Priest, France); Kolliphor HS 15 (BASF, Ludwigshafen, Germany); Lipoid S100 (a soybean lecithin containing 94% of phosphatidylcholine, Lipoïd GMBH, Ludwigshafen, Germany); Oleic acid (OA, > 93%, Sigma-Aldrich Co., St Louis, MO, USA); acetonitrile HPLC grade (Thermo Fisher Scientific, Waltham, MA, USA); and Span ® 80 (extra pure, LobaChemie for Laboratory Reagents and Fine Chemicals, Mumbai, India). All other chemicals were of analytical grade.
5
Caco-2 Cell Culture and Surfactant Assay
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Caco-2 human colonic cancer cells were obtained from the American Type Culture Collection (ATCC; Manassas, Virginia) and were used at passages 35 -50. Cells were cultured in EMEM (Sigma-Aldrich) supplemented with 10% (v/v) FBS (Sigma-Aldrich), 0.1 mg/ml streptomycin, 100 units/ml penicillin, 0.25 µg/ml amphotericin (Sigma-Aldrich) and 2 mM L-glutamine (Sigma-Aldrich) at 37°C with 5% CO2. Unless otherwise stated, cells were seeded at a density of 1 x 10 4 cells per well in 96-well plates (Corning) for 48 hours prior to assay. For the majority of assays the culture media was removed, cells washed twice with phosphate buffered saline (PBS; Sigma-Aldrich) and replaced with Kolliphor® HS15 (BASF) solutions at the required concentrations (0.01, 0.1, 1.0, 10.0, 20.0 or 50.0 mM; concentrations used were selected to be above and below the surfactant CMC 21 ), or the positive control 1.0% (v/v) Triton X-100 (TX; Sigma-Aldrich). All treatment solutions were prepared by direct solvation in Hank's Balanced Salt Solution (HBSS, pH 7.4, Sigma-Aldrich) buffered with 1% HEPES (Biochrom). The negative control in all experiments was 1% HEPES:HBSS solution.
6
Formulation and Evaluation of Dexamethasone Eye Drops
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NAR was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China), glycerol triacetate was bought from J&K Scientific Ltd. (Beijing, China), oleic acid was obtained from Tokyo Chemical Industries Co., Ltd. (Tokyo, Japan), propylene glycol dicaprylate and castor oil were purchased from Hunan Er-Kang Pharmaceutical Co., Ltd. (Changsha, China), PEG-40 hydrogenated castor oil (Cremophor RH40, CRH40), Cremophor EL35, and Kolliphor HS15 were bought from BASF SE (Ludwigshafen, Germany). Tween 80 was purchased from Sichuan Jinshan Pharmaceutical Co., Ltd. (Guangyuan, China), PEG400 was obtained from Solarbio Life Science (Beijing, China), and dexamethasone (DXMS) sodium phosphate eye drops were obtained from Xinxiang Huaqing Pharmaceutical Co., Ltd. (Xinxiang, China). The other reagents and solvents were analytical grade and high-performance liquid chromatography (HPLC) grade, respectively.
7
Formulation Development of Lipid-based Systems
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Disulfiram (CAS number 97-77-8), Kolliphor RH40, Myrj 52, oleic acid, Tween 20, and Tween 80 were purchased from Sigma-Aldrich (Saint Louis, WI, USA). Apifil, Capryol 90, Capryol PGMC, cetylpalmitate, Compritol 888 ATO, Labrafac Lipophile WL 1349, Labrafac PG, Lauroglicol 90, Geleol FPF, Geleol mono/dyglicerides NF, Geloil SC, Labrafil M 2125 CS, Labrafil 1944 CS, Labrasol, Labrasol ALF, Monosteol, Precirol Ato 5, Softisan 601, Suppocire CM, Suppocire DM, Suppocire NB, Suppocire CS2X, and Transcutol HP were kindly gifted by Gatefossé (Lyon, France). Capmul MCM and Mygliol 812 N were provided by Abitec (Columbus, OH, USA). Dynasan 116, Dynasan 118, Inwitor 900 F, Witepsol E76, and Witepsol E85 were donated by IOI Oleochemical (Hamburg, Germany). Lipoid S75 was gifted by Lipoid GmbH (Ludwigshafen, Germany). Kolliwax CA, Kolliwax CSA, Kolliwax GMS II, Kolliwax S, Kolliphor P188, Kolliphor ELP, Kolliphor HS15, and Tween 60 were provided by BASF (Ludwigshafen, Germany). Squalene and Squalane were acquired from EFP Biotek (Figueira da Foz, Portugal). Tween 40, Span 20, Span 40, Span 60, and Span 80 were gifted by SEPPIC SA (Paris, France). Water (Ω = 18.2 MΩ.cm, TOC < 1.5 µg/L) was ultrapurified (Sartorius®, Gottingen, Germany) and filtered through a 0.22 µm nylon filter prior to use. All the other reagents were analytical or High Performance Liquid Chromatography (HPLC) grade.
8
Preparation and Characterization of Lipid-Based Nanoparticles
9
Formulation Development of Alectinib Hydrochloride
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Alectinib hydrochloride was purchased from Wuhan Vanz Pharm Inc. (Wuhan, China). Kolliphor HS 15 (polyoxyl 15 hydroxy stearate), Kollisolv PEG 300 (polyethylene glycol 300), Kollisolv PEG 400 (polyethylene glycol 400), Kolliphor EL (polyoxyl 35 castor oil), Kolliphor PS 20 (Polysorbate 20), Kolliphor PS 80 (polysorbate 80), Kolliphor RH 40 (polyoxyl 40 hydrogenated castor oil), and Kollisolv MCT 70 (medium-chain capric triglyceride) were kindly provided by BASF (Ludwigshafen, Germany). Transcutol P (diethylene glycol monoethyl ether) and Capryol 90 (propylene glycol monocaprylate (type II)) were kindly provided by Gattefosse (Saint-Priest, France). Capmul MCM C8 (mono/diglycerides of caprylic acid) and Capmul MCM C10 (mono/diglycerides of capric acid) were kindly provided by ABITEC Corporation (Janesville, WI, USA). Sodium lauryl sulfate (SLS) was kindly provided by DUKSAN Pure Chemical (Ansan, Korea). All other chemicals were of reagent grade and were thus used without further purification.
10
Development of a Cholinesterase Inhibitor Assay
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Kolliphor® HS 15 (macrogol 15 hydroxystearate) was a gift from BASF (Ludwigshafen, ermany). Labrafac® WL 1349 and Transcutol® HP were kindly provided by Gattefossé S.A. (Saint-Priest, France). Tegaserod was purchased from Synthenova SAS (Hérouville-Saint-Clair, France). The peptide-22 was synthesized by the peptide team of the Institut des Biomolécules Max Mousseron (IBMM, Montpellier, France) according to Malcor et al. [39 (link)]. Acetylthiocholine iodide (ATC), butyrylthiocholine iodide and 5,5-dithiobis-(2-nitrobenzoic) acid (DTNB), human acetylcholinesterase (AChE), equine butyrylcholinesterase (BuChE), donepezil, Triton® X-100 (octylphenol PEG-10 ether), KH2PO4, KCl and NaH2PO4·H2O were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). Tacrine was provided from Tocris (Lille, France). Prisma Buffer HT pH 7,4, sink brain buffer, corticosterone and theophylline was obtained from pION (Billerica, Etats-Unis, MA, USA). Methanol, acetonitrile, acetone, water of HPLC grade were purchased from Carlo Erba Reagents (Val de Reuil, France). Formic acid, Na2HPO4·12 H2O, Tris hydrochloride, HEPES and DMSO were obtained from Fisher Scientific (Illkirch, France). NaCl was provided by Acros Organics (Geel, Belgium).
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