Anti k8

Manufactured by Developmental Studies Hybridoma Bank

Anti-K8 is a monoclonal antibody produced by the Developmental Studies Hybridoma Bank. It is a laboratory reagent used for the detection and study of the K8 protein, which is a member of the keratin family of intermediate filament proteins.

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Lab products found in correlation

Phalloidin, by Thermo Fisher Scientific (1 mentions) Spinning disk confocal microscope, by Leica camera (1 mentions) Hoechst, by AnaSpec (1 mentions) Anti e cad, by Cell Signaling Technology (1 mentions) α sma, by Agilent Technologies (1 mentions) Leica sp5 confocal, by Leica camera (1 mentions) Leica sp5 confocal microscope, by Leica camera (1 mentions) Biorevo bz 9000 digital microscope, by Keyence (1 mentions) Cytokeratin 5, by Fortrea (1 mentions) Anti k14, by Fortrea (1 mentions) Streptavidin hrp, by Biocare Medical (1 mentions) Vectra imaging system, by PerkinElmer (1 mentions) Warp red chromogen, by Biocare Medical (1 mentions) Inform 1, by PerkinElmer (1 mentions) Mach2 rabbit ap polymer, by Biocare Medical (1 mentions) Deep space black chromogen, by Biocare Medical (1 mentions) Cat hematoxylin, by Biocare Medical (1 mentions)

2 protocols using anti k8

Multimodal Microscopic Analysis of Cellular Markers

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IHC was performed as previously described with: Hoechst (AnaSpec, #AS-83218), anti-E-cad (Cell Signaling, #3195 S or BD biosciences, 610182), anti-VANGL2 (SCBT, #46561), anti-VANGL1 (R&D, #HPA02235), Ki67 (Novus, NB500-170), Cleaved Caspase 3 and pERM (Cell Signaling #9661, #3726), Phalloidin (Invitrogen, #A12381), aSMA (DAKO, #M0851), anti-K8 (Developmental Studies Hybridoma Bank, Troma-1), Cytokeratin 5 (Covance, #PRB-160P), and anti-K14 (Covance, #PRB-155P). Images were collected on a Leica SP5 confocal microscope. Brightfield imaging was performed on a Biorevo BZ-9000 Digital Microscope (Keyence) and confocal microscopy performed on a Nikon C2 Confocal, Leica SP5 confocal and Solamere Spinning Disk Confocal Microscope. 3-dimensional reconstructions were performed using Fiji or NIS-elements.

Smith P., Godde N., Rubio S., Tekeste M., Vladar E.K., Axelrod J.D., Henderson D.J., Milgrom-Hoffman M., Humbert P.O, & Hinck L. (2019). VANGL2 regulates luminal epithelial organization and cell turnover in the mammary gland. Scientific Reports, 9, 7079.

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Immunohistochemical Analysis of Breast Cancer

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K5 and K8 expression were examined in a human breast cancer tissue microarray (TMA) containing patient tumors and normal breast samples (University of Wisconsin Carbone Cancer Center BioBank) (30 (link)). In brief, slides were deparaffinized, hydrated and permeabilized, then incubated with anti-K8 (Developmental Studies Hybridoma Bank, University of Iowa, TROMA-1), 1:50 in antibody diluent, (Da Vinci Green-BioCare Medical), followed by goat anti-rat IgG biotinylated secondary antibody (BioCare Medical), Streptavidin-HRP (BioCare Medical), and Deep Space Black chromogen (BioCare Medical). This was followed by anti-K5 (Covance Antibody Products, cat#PRB-160P), 1:4000, Mach 2 rabbit AP polymer (BioCare Medical) and Warp Red chromogen (BioCare Medical). Slides were counterstained with CAT hematoxylin (BioCare Medical), scanned using the Vectra imaging system (PerkinElmer Life Sciences), and analyzed using InForm 1.4 software (PerkinElmer Life Sciences), as described (30 (link)). Cytokeratin and hematoxylin signals were resolved using Nuance 3.0.2 (PerkinElmer Life Sciences), pseudocolored and merged to generate the images shown.

Shea M.P., O’Leary K.A., Fakhraldeen S.A., Goffin V., Friedl A., Wisinski K.B., Alexander C.M, & Schuler L.A. (2018). Anti-estrogen therapy increases plasticity and cancer stemness of prolactin-induced ERα+ mammary carcinomas. Cancer research, 78(7), 1672-1684.

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