Ftir spectrophotometer

Manufactured by PerkinElmer

Sourced in United States, United Kingdom, Japan, Germany

The FTIR spectrophotometer is a laboratory instrument designed to analyze the composition of materials by measuring their infrared absorption spectrum. It functions by passing infrared radiation through a sample and detecting the wavelengths at which the sample absorbs the radiation. The resulting spectrum provides information about the chemical bonds and molecular structure of the sample.

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Lab products found in correlation

Nmr spectrophotometer, by Bruker (11 mentions) Model 2400 chnso analyser, by PerkinElmer (8 mentions) Model 2400 chnso analyzer, by PerkinElmer (5 mentions) Jem 2100f, by JEOL (4 mentions) Sta 6000, by PerkinElmer (3 mentions) D5000 diffractometer, by Siemens (3 mentions) Avance 2 400 nmr spectrometer, by Bruker (3 mentions) Silica gel 60 f254 coated plates, by Merck Group (3 mentions) Model no jsm 7610f, by JEOL (2 mentions) Smartlab se x ray diffraction analyzer, by Rigaku (2 mentions) Dr 1800 uv visible spectrometer, by Shimadzu (2 mentions) Jsm 6510lv sem machine, by JEOL (2 mentions) Uv 1800, by Shimadzu (2 mentions) Jsm 6510lv, by JEOL (2 mentions) Chns 932 elemental analyser, by Leco (2 mentions) Start s microwave labstation for synthesis, by Milestone (2 mentions) Jsm 7610f, by JEOL (2 mentions) Sabouraud dextrose broth (sdb), by HiMedia (2 mentions) Nutrient broth, by HiMedia (2 mentions) Nutrient agar, by HiMedia (2 mentions)

Spelling variants of this product

1600 FTIR spectrophotometer (12 citations) Fourier transform infrared (FTIR) spectrophotometer (9 citations) Spectrum 2000 FTIR spectrophotometer (5 citations) FTIR Spectrum One spectrophotometer (5 citations) Spectrum 400 FTIR spectrophotometer (5 citations) Paragon 1000 FTIR spectrophotometer (4 citations) FTIR 2000 spectrophotometer (3 citations) FTIR spectrophotometer 1000 (3 citations) Nicolet 6700 FT-IR Perkin Elmer 16F PC FTIR spectrophotometer (2 citations) UTAR-FTIR spectrophotometer (2 citations)

224 protocols using ftir spectrophotometer

Characterization of Ginsenoside-Loaded Nanocomposites

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Example 12

The amount of ginsenoside loaded in the MSNP-CK or MSNP-Rh2 nanocomposite was calculated by HPLC.

As a result, as shown in FIG. 32A, MSNP-CK showed a clear peak consistent with that of CK alone, and the amount of CK loaded in MSNP-CK was 0.066 mg per 1 mg of nanocomposite. Furthermore, as shown in FIG. 32D, MSNP-Rh2 showed a clear peak consistent with that of Rh2 alone, and the amount of Rh2 loaded in MSNP-Rh2 was 0.072 mg per 1 mg of nanocomposite.

In order to examine whether the MSNP-CK and MSNP-Rh2 nanocomposites of the present invention were prepared well, structures of MSNP-CK, MSNP-Rh2, and intermediates thereof were examined by ¹H NMR and FT-IR.

Specifically, ¹H NMR spectra were recorded at 300 MHz (JEOL, Tokyo, Japan), and samples were dissolved in D₂O or CD₃OD. FT-IR spectra were confirmed by a Perkin-Elmer FT-IR spectrophotometer using KBr pellets.

As a result, as shown in FIGS. 32B and 32C, ¹H NMR and FTIR results of MSNP-CK showed a characteristic peak of CK. Further, as shown in FIGS. 32E and 32F, ¹H NMR and FTIR results of MSNP-Rh2 showed a characteristic peak of Rh2.

These results indicate that the MSNP-CK and MSNP-Rh2 nanocomposites have intrinsic characteristics of CK and Rh2, respectively.

US11648208B2. Nano complex comprising a nano drug delivery matrix; and a ginseng extract or a ginsenoside isolated therefrom (2023-05-16). University-Industry Cooperation Group of Kyung Hee University [KR]. Inventors: Deok Chun Yang [KR], Yeon-Ju Kim [KR], Singh Priyanka [KR], Dong Uk Yang [KR], Sung Eun Ahn [KR], Mathiyalagan Ramya [KR].

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FTIR Analysis of Resveratrol Nanofibers

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The samples (pure resveratrol, PVP, HPBCD and different ratio formulations of resveratrol nanofibers) were mixed with potassium bromide using a mortar, and compressed to form thin sheets. The samples were then analyzed by a FTIR spectrophotometer (Perkin-Elmer Inc., Waltham, MA, USA).

Lin Y.C., Hu S.C., Huang P.H., Lin T.C, & Yen F.L. (2020). Electrospun Resveratrol-Loaded Polyvinylpyrrolidone/Cyclodextrin Nanofibers and Their Biomedical Applications. Pharmaceutics, 12(6), 552.

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Characterization of Novel Compounds

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All of the materials and solvents used in this experiment were classified as analytical reagent grade unless otherwise specified. Thin-layer chromatography (TLC) was conducted on glass plate silica gel 60 F254 (Merck KGaA, Darmstadt, Germany) then visualized by UV 254 and 366 nm. Proton nuclear magnetic resonance (¹H-NMR) and carbon-13 (¹³C)-NMR spectra were recorded on a JMTC-400/54/SS (400 MHz, JEOL Ltd., Tokyo, Japan) spectrometer. Infrared (IR) spectra were determined as KBr pellets of the solids on an FT-IR spectrophotometer (Perkin Elmer). Waltham, Massachusetts, USA).ESI–MS measurements were conducted by using a XevoQTof (Waters Corporation, Massachusetts, US). Flash chromatography was performed using a Biotage system (Biotage, Sweden) and melting points were determined using a melting point apparatus (BUCHI Labortechnik AG, Flawil, Switzerland).

Susidarti R.A., Utomo R.Y., Qodria L., Ramadani R.D., Ohta Y., Hattori Y., Kirihata M, & Meiyanto E. (2019). Preparation of pentagamaboronon-0 and its fructose and sorbitol complexes as boron carrier for boron neutron capture therapy (BNCT) application. Research in Pharmaceutical Sciences, 14(4), 286-292.

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Comprehensive Characterization of Nanoparticles

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The surface Morphology of the nanoparticles was studied by a Scanning Electron Microscope (Model no. JSM-7610F from JEOL Ltd., Tokyo, Japan) at an accelerating voltage of 15 kV. Fourier-transform infrared (FTIR) spectra of the materials were taken on a PerkinElmer FTIR spectrophotometer, model number FTIR-100. Thermogravimetric analysis (TGA) of the materials was conducted on a PerkinElmer STA-6000 instrument at a heating rate of 10 °C/min under an N₂ gas environment. The XRD of the samples was taken on a Rigaku, SmartLab-SE X-Ray Diffraction Analyzer. A Shimadzu (Kyoto, Japan) DR 1800 UV-visible spectrometer was used for optical absorbance measurement as a function of the wavelength in the range of 200–800 nm. A centrifuge machine (Model no. BKC-TH1611, Biobase Corporation, Jinan, China) operated at 10,000 rpm was used for the purification of the nanoparticles by repeated washing.

Islam M.T., Roni M.N., Ali M.Y., Islam M.R., Hossan M.S., Rahman M.H., Zahid A.A., Alam M.N., Hanif M.A, & Akhtar M.S. (2023). Selectivity of Sol-Gel and Hydrothermal TiO2 Nanoparticles towards Photocatalytic Degradation of Cationic and Anionic Dyes. Molecules, 28(19), 6834.

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Characterization of 4-Chloro-2-Phenylquinazoline Derivatives

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Melting points (uncorrected) were determined in open capillaries on a Gallen Kamp melting point apparatus (Gallemkamp, MSE Ltd., London, UK). Precoated silica gel plates (Kieselgel 0.25 mm, 60 G F254; EMD Millipore, Billerica, MA, USA) were used for thin layer chromatography. A solvent system of chloroform/methanol mixture (8:2 mL) was used as a developing system, and the spots were detected by ultraviolet light. Infrared (IR) spectra (KBr disk) were recorded using a Perkin Elmer FT-IR spectrophotometer (PerkinElmer Inc., Waltham, MA, USA). Nuclear magnetic resonance (NMR) spectra were scanned on a Bruker NMR spectrophotometer (Bruker AXS Inc., Madison, WI, USA), operating at 500 MHz for ¹H spectra and 125.76 MHz for ¹³C spectra. Chemical shifts are expressed in δ-values (ppm) relative to tetramethylsilane as an internal standard, using dimethyl sulfoxide (DMSO)-d₆ as a solvent. Elemental analyses were done on a model 2400 Perkin Elmer CHNSO analyzer (PerkinElmer Inc.). All the values were within ±0.4% of the theoretical values. All reagents used were of analytical reagent grades. The starting material 4-chloro-2-phenylquinazoline 1 was purchased from Sigma-Aldrich Co. (St Louis, MO, USA) and was directly used for preparation of the target compounds.

Ghorab M.M., Alsaid M.S., Higgins M., Dinkova-Kostova A.T., Shahat A.A., Elghazawy N.H, & Arafa R.K. (2016). NAD(P)H:quinone oxidoreductase 1 inducer activity of some novel anilinoquinazoline derivatives. Drug Design, Development and Therapy, 10, 2515-2524.

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Cryogel Chemical Composition Analysis

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The cryogels were analysed using Fourier transform infrared (FT-IR) spectrophotometer (Perkin Elmer) in order to study their chemical composition. The analysis was carried out in the ATR mode, by compressing the samples until a clear spectrum was shown in the screen and the transmittance peaks were then registered. Every sample was analysed with a resolution of 4 cm⁻¹ and an OPD of 0.2. In total, 100 scans were performed to obtain each graphic.

Vilela M.J., Colaço B.J., Ventura J., Monteiro F.J, & Salgado C.L. (2021). Translational Research for Orthopedic Bone Graft Development. Materials, 14(15), 4130.

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Characterization of Synthesized ZnO Nanoparticles

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The synthesis of ZnO NPs at the preliminary level was confirmed by recording the absorbance (A) on a UV-vis spectrophotometer (Perkin Elmer Life and Analytical Sciences, CT, USA) in the wavelength range of A₂₀₀ to A_{800 nm} [37 ]. Further, authentication of the successful synthesis of ZnO NPs was done by X-ray diffraction (XRD) pattern. For that, the ZnO NPs powdered sample was recorded on MiniFlex II benchtop XRD system (Rigaku Corporation, Tokyo, Japan) operating at 40 kV [40 (link)]. The surface morphology of ZnO NPs was determined using a JEOL- JSM-6510LV SEM machine (Tokyo, Japan) operated at a voltage of 10 kV. The transmission electron microscopy (TEM) of ZnO NPs was carried out on JEOL 100/120 kV TEM (JEOL, Tokyo, Japan) with an accelerating voltage of 200 kV. The lattice fringes and diffraction ring patterns (SAED) were examined in its high resolution (HRTEM) mode. FTIR spectra of ZnO NPs were obtained in the range 4,000 to 400 cm^-1 with a PerkinElmer FTIR spectrophotometer by potassium bromide (KBr) pellet method [37 , 40 (link)]. Spectroscopic grade KBr was used in the ratio of 1:100 and spectrum was recorded in the diffuse reflectance mode at a resolution of 4 cm^-1 in KBr pellets.

Khan Y.A., Singh B.R., Ullah R., Shoeb M., Naqvi A.H, & Abidi S.M. (2015). Anthelmintic Effect of Biocompatible Zinc Oxide Nanoparticles (ZnO NPs) on Gigantocotyle explanatum, a Neglected Parasite of Indian Water Buffalo. PLoS ONE, 10(7), e0133086.

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FTIR Analysis of Drug-Loaded Niosomes

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Lyophilized samples of Epi-Nio-HA and all the other elements of drug-loaded niosomes were mixed with KBr in the form of pellet by a hydraulic strain and then analyzed using PerkinElmer FTIR spectrophotometer (spectrum Two, USA). The procedure was performed in the scanning range of 4000–400 cm⁻¹ in a constant resolution of 4 cm⁻¹.

Mansoori-Kermani A., Khalighi S., Akbarzadeh I., Niavol F.R., Motasadizadeh H., Mahdieh A., Jahed V., Abdinezhad M., Rahbariasr N., Hosseini M., Ahmadkhani N., Panahi B., Fatahi Y., Mozafari M., Kumar A.P, & Mostafavi E. (2022). Engineered hyaluronic acid-decorated niosomal nanoparticles for controlled and targeted delivery of epirubicin to treat breast cancer. Materials Today Bio, 16, 100349.

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Spectroscopic Characterization of Organic Compounds

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Dry dichloromethane (DCM) was dried following the standard method. The IR-spectra (cm⁻¹) were taken with a FT-IR spectrophotometer (Perkin Elmer, USA) using attenuated total reflection sampling technique (ATR). NMR spectra were taken using a Bruker spectrometer (¹H, 400 MHz, ¹³C, 100 MHz, DMSO-d₆), and chemical shifts (δ) are reported in parts per million (ppm). The Flash elemental analyzer connected with thermal conductivity detector (Flash-TCD 2000 Series, USA) was used to perform elemental analysis (C, H) and reported uncorrected with ±0.3%. The melting points determined by Digimelt MPA 160 (USA) are reported uncorrected. Silica gel thin layer column chromatography-TLC was used to check the purity of title derivatives (petroleum spirit : ethyl acetate, 2 : 1). All other reagents such as the enzyme mushroom tyrosinase (Mtyr, EC 1.14.18.1), L-DOPA, and 1-(3-acetylphenyl)ethan-1-one were analytical grade (Sigma Aldrich, USA) and are used without further purification.

Nazir Y., Rafique H., Roshan S., Shamas S., Ashraf Z., Rafiq M., Tahir T., Qureshi Z.U., Aslam A, & Asad M.H. (2022). Molecular Docking, Synthesis, and Tyrosinase Inhibition Activity of Acetophenone Amide: Potential Inhibitor of Melanogenesis. BioMed Research International, 2022, 1040693.

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FTIR-ATR Spectroscopy Study Protocol

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Infrared spectroscopy study was performed on FTIR spectrophotometer (Perkin ELMER, Ltd., London, UK) in the attenuated total reflection mode (ATR). The FTIR-ATR spectra were recorded at the 4000–400 cm⁻¹ interval, by collecting 16 scans for each point, at a spectral resolution of 4 cm⁻¹.

Căprărescu S., Modrogan C., Purcar V., Dăncilă A.M, & Orbuleț O.D. (2021). Study of Polyvinyl Alcohol-SiO2 Nanoparticles Polymeric Membrane in Wastewater Treatment Containing Zinc Ions. Polymers, 13(11), 1875.

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