Il 1β

Manufactured by Mesoscale

Sourced in United States

IL-1β is a cytokine that plays a crucial role in the inflammatory response. It is a key mediator of the body's immune response to infection, injury, and other stimuli. IL-1β is involved in the activation of various immune cells and the regulation of inflammatory processes.

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Lab products found in correlation

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Close spelling variants of this product

V-PLEX Mouse IL-1β Kit U-PLEX Mouse IL-1β Assay Mouse IL-1β tissue culture kit

5 protocols using il 1β

Multiplex Cytokine Profiling

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We performed multiplex assays to measure IL-1β, IL-2, IL-6, TNFα, and IFN-γ purchased from Meso Scale Discovery. All experimental operations were in accordance with standard protocols. R2s of the standard curves for each plate were greater than 0.999.

Bremner J.D., Gurel N.Z., Jiao Y., Wittbrodt M.T., Levantsevych O.M., Huang M., Jung H., Shandhi M.H., Beckwith J., Herring I., Rapaport M.H., Murrah N., Driggers E., Ko Y.A., Alkhalaf M.L., Soudan M., Song J., Ku B.S., Shallenberger L., Hankus A.N., Nye J.A., Park J., Vaccarino V., Shah A.J., Inan O.T, & Pearce B.D. (2020). Transcutaneous vagal nerve stimulation blocks stress-induced activation of Interleukin-6 and interferon-γ in posttraumatic stress disorder: A double-blind, randomized, sham-controlled trial. Brain, Behavior, & Immunity - Health, 9, 100138.

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Comprehensive Cardiometabolic Biomarker Profiling

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Blood lipids were measured by LabCorp (Burlington, NC). Commercially available multiarray kits were used to quantify plasma levels of interleukin 1β (IL-1β), IL-6, IL-8, tumor necrosis factor alpha (TNFα), C-reactive protein (CRP), soluable vascular cell adhesion molecule 1 (sVCAM1), soluable intercellular adhesion molecule 1 (sICAM1), serum amyloid A (SAA), tissue-type plasminogen activator (tPA), plasminogen, D-dimer, and von Willebrand factor (vWF) (Meso-Scale Diagnostics, Gaithersburg, MD). In addition, vasoconstrictor endothelin-1 (ET-1) and oxidized low-density lipoprotein (Ox-LDL) were measured using kits purchased from Bachem (Torrence, CA) and Mercodia (Winston Salem, NC), respectively. All assays were carried out according to the manufacturer’s instructions.

Chen H., Zhang S., Yu B., Xu Y., Rappold A.G., Diaz-Sanchez D., Samet J.M, & Tong H. (2022). Circulating microRNAs as putative mediators in the association between short-term exposure to ambient air pollution and cardiovascular biomarkers. Ecotoxicology and environmental safety, 239, 113604.

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Profiling Immune Cytokine Responses

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1.5 × 10⁶ Jurkat, THP1 (ATCC), THP1-Dual or THP1-Dual STING KO cells were treated with 3x GI₅₀ V158411 or 50 μg/mL cGAMP for 24 h. Cells were harvested by centrifugation and lysed in 100 μL ice cold lysis buffer on ice for 30 min. Cell debris was pelleted and 25 μL of the supernatant added to a 10 spot U-PLEX biomarker plate pre-conjugated with anti-TNF-α, MCP-3, IL-6, IL-22, IL-18, IL-1β, IL-1α, IFN-γ, IFN-β and IFN2a capture antibodies (MesoScale Discovery, Rockville, MD). The plate was developed according to the manufacturer’s instructions and read using a SECTOR Imager 2400.

Brooks T., Wayne J, & Massey A.J. (2021). Checkpoint Kinase 1 (Chk1) inhibition fails to activate the Stimulator of Interferon Genes (STING) innate immune signalling in a human coculture cancer system. Molecular Biomedicine, 2, 19.

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Intradermal LPS Administration Safety and Cytokine Response

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Safety and tolerability were monitored by tracking adverse events, measuring vital signs, and standard laboratory tests (i.e. haematology) at 3, 6, 10, 24 and 48 hours after LPS administration. Circulating cytokines (IL‐1β, IL‐6, IL‐8, IL‐10, IFN‐γ and TNF; Meso Scale Discovery, Rockville, Maryland, USA) were measured in blood samples to detect a possible systemic effect of intradermal LPS administration. The blood samples for cytokine analysis were analysed in 2 batches with each having a different dilution, therefore the lower limit of quantitation (LLOQ) of the samples was the following: IL‐1β 0.298 or 0.745 pg/mL; IL‐6 1.52 or 3.81 pg/mL; IL‐8 1.25 or 3.12 pg/mL; IL‐10 0.702 or 1.76 pg/mL; IFN‐γ 10 or 25 pg/mL; and TNF 0.760 or 1.90 pg/mL.

Buters T.P., Hameeteman P.W., Jansen I.M., van Hindevoort F.C., ten Voorde W., Florencia E., Osse M., de Kam M.L., Grievink H.W., Schoonakker M., Patel A.A., Yona S., Gilroy D.W., Lubberts E., Damman J., Feiss G., Rissmann R., Jansen M.A., Burggraaf J, & Moerland M. (2021). Intradermal lipopolysaccharide challenge as an acute in vivo inflammatory model in healthy volunteers. British Journal of Clinical Pharmacology, 88(2), 680-690.

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BALF Cytokine Profiling via MSD

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The BALF supernatant samples were analysed using MSD pro-inflammatory panel V-Plex including the following analytes: IFN-γ, IL-10, IL-12p70, IL-1β, IL-2, IL-4, IL-5, IL-6, KC/GRO and TNF-α (Meso Scale Diagnostics, Rockville, MD). The analysis was performed according to the manufacturer’s instruction.

Wronski S., Dannenmaier J., Schild S., Macke O., Müller L., Burmeister Y., Seilheimer B, & Müller M. (2018). Engystol reduces onset of experimental respiratory syncytial virus-induced respiratory inflammation in mice by modulating macrophage phagocytic capacity. PLoS ONE, 13(4), e0195822.

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