Axio examiner z1

Male and female C57BL/6J mice (n = 6) were intravenously injected with vehicle control (equivalent volume of saline), ML355 (1.5 mg/kg), sHDL (50 mg/kg), or ML355-sHDL (sHDL at 50 mg/kg and ML355 at 1.5 mg/kg), respectively. Twenty-four hours after administration, mice were anesthetized as described above and tail veins were injected with DyLight 488 anti-GPIb (1 μg/g; Emfret, EIbelstadt, Germany) to label circulating resting platelets in mice before intravital microscopy imaging. The mice were placed on a heating pad, and the right common carotid artery was prepared under the dissecting microscope. Then, the mice were placed on the microscopic stage and blood flow in the carotid artery was visualized under 10× air objective using a Zeiss Axio Examiner Z1 upright fluorescence microscope. Carotid artery injury was induced by topically placing a 10% FeCl₃ saturated Whatman paper for 3 min under recording. Images of platelet adhesion and the dynamics of thrombus formation were recorded for 30 min using a high-speed sCMOS camera using SlideBook 6.0. Vessel occlusion was defined by formation of an occlusive thrombus, and cease of blood flow for 1 or 30 min was taken as the vessel occlusion time if the carotid artery failed to occlude during the recording.

Our experiments were conducted according to the European and Institutional guidelines [Council Directive 86/609/EEC and French National Research Council and approved by the local health authority (Préfecture des Bouches-du-Rhône, Marseille)]. Neocortical slices (350 to 400 μm) were obtained from 14- to 49-day-old Wistar rats of both sexes. Rats were deeply anesthetized with chloral hydrate (intraperitoneal, 200 mg/kg) and killed by decapitation. For animals older than P40, rats were deeply anesthetized with isoflurane and ice-cold perfused with the slicing solution (see composition below) and killed by decapitation. Slices were cut in an ice-cold solution containing 92 mM n-methyl-d-glutamine, 30 mM NaHCO₃, 25 mM d-glucose, 10 mM MgCl₂, 2.5 mM KCl, 0.5 mM CaCl₂, 1.2 mM NaH₂PO₄, 20 mM Hepes, 5 mM sodium ascorbate, 2 mM thiourea, and 3 mM sodium pyruvate and were bubbled with 95% O₂–5% CO₂ (pH 7.4). Slices recovered (1 hour) in a solution containing 125 mM NaCl, 26 mM NaHCO₃, 2 mM CaCl₂, 2.5 mM KCl, 2 mM MgCl₂, 0.8 mM NaH₂PO₄, and 10 mM d-glucose and were equilibrated with 95% O₂–5% CO₂. Each slice was transferred to a submerged chamber mounted on an upright microscope (Olympus BX51WI or Zeiss Axio-Examiner Z1), and neurons were visualized using differential interference contrast infrared videomicroscopy.

The
channel dimensions were measured by using a Zeiss Axio Examiner Z1
microscope. The thickness of the CNT mesh was observed by using scanning
electron microscopy (SEM) cross-sectional imaging. The compressive
strain effect was observed using Raman spectroscopy, which was performed
using an alpha300 apyron microscope with a 532 nm laser. The electronic
properties of the CNTs, including carrier density, resistivity, and
mobility, were measured using a Lake Shore Hall measurement system
(Model 7704A). Porosimetry tests were conducted using a high-resolution
3Flex 3500 (Micromeritics) adsorption instrument with three 0.1 Torr
pressure transducers and a high-vacuum system. The measurement was
performed at −196 °C using a liquid N₂ bath,
while sorption measurements were conducted using ultrahigh-purity
nitrogen gas (99.999%). The samples, having a weight of ∼200
mg, were degassed for 12 h under a strong vacuum of 1 μ torr
at room temperature before testing. Using the multipoint Brunauer–Emmett–Teller
method, the specific surface area was extracted in the relative pressure
range of 0.01 < P/Po < 0.1. The porosity was
measured before and after heating for 3 min at 110 °C via mercury
intrusion porosimetry (MicroActive AutoPore V 9600 2.03.00, USA).