Sodium chloride (NaCl), calcium chloride (CaCl2) potassium phosphate monobasic (KH2PO4), potassium phosphate dibasic (K2HPO4), sodium hydroxide (NaOH), sodium phosphate dibasic (Na2HPO4), sodium hypochlorite (NaClO) solution 10% w/v, dimethyl sulfoxide (DMSO), acetonitrile (ACN), hydrochloric acid (HCl), methanol (MeOH), trifluoroacetic acid (TFA) and tert-butyl hydroperoxide (tBuOOH) were purchased from Panreac (Barcelona, Spain). Agar, cholesterol, sodium ampicillin, nystatin, bacto-yeast, 5-fluoro-2′-deoxyuridine (FUdR), phosphate buffered saline and 2’,7’-dichlorofluorescein diacetate (DFCH-DA) were purchased from Sigma-Aldrich (Madrid, Spain). Peptone enzymatic digest from soybean, bacto-tryptone and trichloroacetic acid (TCA) were provided by Fluka (Madrid, Spain). Magnesium sulphate, tween 20 (poly-oxy-ethyl-ensorbitan-monolaurate) and acetic acid were acquired from Merck (Darmstadt, Germany). Water (H2O) was purified by using a Milli-Q Plus185 system from Millipore (Milford, MA, USA). Reversed-phase C18 resin (12–20 µm) was purchased from Sigma-Aldrich.
Sodium chloride (nacl)
Manufactured by ITW Reagents
Sourced in Spain, United States, Germany, Switzerland
Sodium chloride is a chemical compound that is commonly known as table salt. It is a white, crystalline solid that is widely used in various industries, including the laboratory setting. Sodium chloride has a high solubility in water and is an essential component in many scientific experiments and analytical procedures.
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Lab products found in correlation
Sodium hydroxide (naoh), by ITW Reagents (15 mentions)
Potassium chloride (kcl), by ITW Reagents (14 mentions)
Hydrochloric acid (hcl), by ITW Reagents (11 mentions)
Methanol, by ITW Reagents (9 mentions)
Milli q system, by Merck Group (9 mentions)
Methanol, by Merck Group (8 mentions)
Dimethyl sulfoxide (dmso), by ITW Reagents (7 mentions)
Glacial acetic acid, by ITW Reagents (6 mentions)
Kh2po4, by ITW Reagents (6 mentions)
Galantamine, by Merck Group (6 mentions)
Tyrosinase, by Merck Group (6 mentions)
4 aminoantipyrine, by Merck Group (6 mentions)
Horseradish peroxidase, by Merck Group (6 mentions)
Sodium carbonate, by ITW Reagents (5 mentions)
Potassium dihydrogen phosphate, by Merck Group (5 mentions)
Acetic acid, by Merck Group (5 mentions)
Formic acid, by Merck Group (5 mentions)
Sodium carbonate, by Merck Group (5 mentions)
Bovine serum albumin (bsa), by Merck Group (5 mentions)
Tyramine, by Merck Group (5 mentions)
119 protocols using sodium chloride (nacl)
1
Analytical Reagents and Solutions Protocol
2
In Vitro Digestion Protocol
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Ethanol, sodium phosphate, potassium phosphate, acetonitrile, sodium hydroxide, trichloroacetic acid, hydrochloric acid, gallic acid, potassium ferricyanide, ferric chloride and calcium chloride dihydrate were purchased from Fisher Scientific (Pittsburgh, PA, USA). Folin–Ciocalteu’s reagent, potassium chloride, ammonium carbonate, acetic acid glacial and sodium chloride were purchased from Panreac Applichem (Barcelona, Spain). Magnesium chloride hexahydrate and aluminum chloride were from Acros Organics (Pittsburgh, PA, USA), and sodium carbonate anhydrous was purchased from Labkem (Barcelona, Spain).
Human salivary α-amylase (A1031-1KU), porcine pepsin (P6887), porcine pancreatin 4 × USP specifications (P1750), bile bovine (B3883), 2,2-diphenyl-1-picrylhydrazyl (DPPH), quercetin, chrysin and p-coumaric acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). Kaempferol was purchased from Extrasynthese (Genay, France) and naringenin from Acros Organics (Pittsburgh, PA, USA).
Human salivary α-amylase (A1031-1KU), porcine pepsin (P6887), porcine pancreatin 4 × USP specifications (P1750), bile bovine (B3883), 2,2-diphenyl-1-picrylhydrazyl (DPPH), quercetin, chrysin and p-coumaric acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). Kaempferol was purchased from Extrasynthese (Genay, France) and naringenin from Acros Organics (Pittsburgh, PA, USA).
3
Synthesis of Multifunctional Magnetic Nanoparticles
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Iron(III) chloride hexahydrate (FeCl3.6H2O, 97.0−102.0%), iron(II) chloride hydrate (FeCl2.H2O, 99%), ammonium nitrate (NH4NO3, 99.999%), N-hydroxysuccinimide (NHS, 98+%), ethylenediaminetetraacetic acid tetrasodium salt hydrate (EDTA, 98%) and tetraethyl orthosilicate (TEOS, ≥99.999% metals basis) were bought at AlfaAesar. The n-Cetyltrimethylammonium bromide (CTAB), (3-aminopropyl) triethoxysilane (APTES, ≥98%), thioglycolic acid (≥99.9%), succinic anhydride (≥99% GC), (+)-sodium L-ascorbate (crystalline, ≥98%), N-3-dimethylaminopropyl-n-ethylcarbodiimide hydrochloride (EDC, ≥97%), acetone (≥99.5% GC), 4-morpholineethanesulfonic acid (MES, ≥99%), TRIS (hydroxymethyl)aminomethane hydrochloride (TRIS HCl) and phosphate-buffered saline (PBS, tablets) were purchased from Sigma Aldrich. Ethanol absolute (EtOH, extra pure), hydrochloric acid (HCl, 37%) and sodium hydroxide (NaOH) were bought at Scharlab, SL. Oleic acid (OA, 65.0–88.0 %) was acquired from Honeywell Fluka and sodium chloride (NaCl, 99.0–100.5%) at PanReac AppliChem. BactoTM Glycerol was bought from Becton Dickinson&Co (Sparks, MD, USA). Muller Hinton Broth (MHB) and Trypto Casein-Soy Broth (TSB) were obtained from Biokar Diagnostics. All reagents were used as acquired, without any further purification, and all solutions, unless otherwise indicated, were prepared with deionized Millipore miliQ water.
4
Curcumin Mediated Peptide Interactions
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Trizma (Tris base), tricine, sodium dodecyl sulfate, sodium phosphate dibasic heptahydrate, sodium phosphate monobasic monohydrate, sodium acetate, acetic acid, and imidazole were obtained from Sigma-Aldrich. Sodium chloride and sodium hydroxide were obtained from PanReac, hydrochloric acid and 99% ethanol from VWR, and 98% curcumin was obtained from Acros Organics. Acrylamide solutions were purchased from Bio-Rad, and ammonium persulfate (APS) and “N-N-N′-N′-” tetramethylethylenediamine (TEMED) were acquired from NZYTech. The protopeptide (YMDMSGYQ sequence) was chemically synthesized using free terminals and TFA-free at 97% purity by GeneCust (France). Mica used for AFM assays—G250-2 Mica sheets (Cat# AGG250-3, Agar Scientific).
5
Quantitative Analysis of Food Volatiles
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All chemicals used had a purity grade higher than 95%. Hexanal, benzaldehyde, 2-methylpropanal, nonanal, 2-methylbutanal, 3-methylbutanal, and 4-fluorobenzaldehyde standards were purchased from Sigma-Aldrich (Steinheim, Germany). Acetaldehyde, phenylAcetaldehyde, 5-(hydroxymethyl) furfural, 2-furaldehyde, and trans-2-nonenal were purchased from Acros Organics (Geel, Belgium). Absolute ethanol, acetonitrile, and methanol (HPLC grade 99.99%) was obtained from Sigma-Aldrich (Steinheim, Germany). Sodium chloride was obtained from Panreac (Barcelona, Spain). Ultra-pure water with a resistivity of >18 MΩ.cm (type 1) was obtained from a Millipore Simplicity® UV apparatus (Milford, MA, USA). The alkane solution (C7–C30) was obtained from Supelco (Sigma Aldrich, St. Louis, MO, USA).
6
Determination of Lipolytic Enzyme Activities
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Pepsin from porcine gastric mucosa, porcine bile extract and pancreatin and lipase from porcine pancreas were obtained from Sigma (St. Louis, MO, USA). Gastric lipase (rabbit gastric extract, RGE 15) was obtained from Lipolytech® (Marseille, France). The protocols described in Brodkorb et al. [28 (link)] were applied to determine the enzyme activities. Dichloromethane, chloroform, hexane, methanol, isooctane, isopropanol, dimethylformamide and acetonitrile, which were all HPLC grade, were purchased from Labscan (Dublin, Ireland). The purified water was obtained from a Milli-Q water purification system (Millipore, Milford, MA, USA). Sodium sulphate anhydrous, sodium chloride, potassium chloride and sodium carbonate were obtained from Panreac (Barcelona, Spain). Sodium methoxide (95%), sodium citrate dehydrate, formic acid (98%), triethylamine (99.5%), acetic acid and trinanoin were supplied by Sigma-Aldrich (St. Louis, MO, USA).
7
Volatile Compound Analysis by GC-MS
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All chemicals used had a purity grade higher than 95%. Hexanal, benzaldehyde, 2-methylpropanal, nonanal, 2-methylbutanal, 3-methylbutanal, and 4-fluorobenzaldehyde standards were purchased from Sigma-Aldrich (Steinheim, Germany). Acetaldehyde, phenylAcetaldehyde, 5-(hydroxymethyl) furfural, 2-furaldehyde and trans-2-nonenal were purchased from Acros Organics (Geel, Belgium). Absolute ethanol, acetonitrile, and methanol (all HPLC grade 99.99%) came from Sigma-Aldrich (Steinheim, Germany). Sodium chloride was obtained from Panreac (Barcelona, Spain). Ultra-pure water with a resistivity of >18 MΩ·cm (type 1) was obtained from a Millipore Simplicity® UV apparatus (Milford, MA, USA). The alkane solution (C7–C30) was obtained from Supelco (Sigma Aldrich, St. Louis, MO, USA).
8
Biomolecular Functionalization Protocol
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The following chemicals and solvents were used: sulfuric acid 98%, methanol, glacial acetic acid, MES buffer, sodium hydroxide, potassium dihydrogen phosphate and 4-methoxy benzaldehyde(p-anisaldehyde from Merck (Darmstadt, Germany); chloroform and ethanol (100%) from Carlo Erba (Val-de-Reuil, France); N-hydroxysuccinimide (NHS), N-(3-dimethylaminopropyl)-N′-ethyl carbodiimide hydrochloride (EDC), D-(+)-glucose monohydrate and (3-aminopropyl) triethoxysilane (APTES) from Sigma-Aldrich (Saint Louis, MO, USA); sodium chloride from Panreac (Barcelona, Spain); acetonitrile and hydrochloric acid 37% from Scharlau (Sentmenat, Spain); hydrogen peroxide 30% from VWR Chemicals (Leuven, Belgium); peptone, agar and yeast extract from Biokar Diagnostics (Beauvais, France); deionized water obtained from a MilliQ system.
9
Synthesis of Metal Chloride Compounds
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All chemical reagents used in this study were of analytical grade and were used without further purification. Ruthenium (III) chloride hydrate (RuCl3xH2O–99.5%), antimony (III) chloride (SbCl3–99.0%), hexachloroplatinic acid (H2PtCl6·6H2O–37.5%), oxalic acid (C2H2O4–99.5%), and isopropanol (C3H8O–99.8%) were purchased from Sigma-Aldrich®. (Burlington, MA, USA). Hydrochloric acid (HCl–38.0%) was acquired from Neon® (Suzana, São Paulo, Brazil), sulfuric acid (H2SO4–97%) from Scharlab® (Sentmenat, Spain), and sodium chloride (NaCl–99.5%) from Panreac® (ITW, Chicago, IL, USA). All solutions were prepared using ultrapure water (18.2 MΩcm, 25 °C) from a Gehaka Master All 2000 System (São Paulo, Brazil).
10
GC-MS Profiling of Volatile Compounds
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Internal standard (IS), 4-heptanone, was purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium chloride was acquired from Panreac (Barcelona, Spain). SPME holder and the fiber DVB/CAR/PDMS (50/30 μm) were acquired from Supelco (Bellefonte, PE, USA). The BP-20 fused silica capillary column (60 m × 0.25 mm I.D. × 0.25 µm) was acquired from SGE (Dortmund, Germany). Ultrapure deionized water (H2O) was obtained from a system from Millipore (Burlington, MA, USA). The Mixer was purchased from Thermo Scientific (Burlington, MA, USA). All methods used in chemometric analysis were performed using the STATSOFT STATISTICA 12.0 (2013) software (Tulsa, OK, USA).
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