Samples were sputter coated with a 10 nm layer of platinum (Q150S, Quorum Technologies) and loaded into a 3View2XP (Gatan, Pleasanton, CA) attached to a Sigma VP SEM (Zeiss) with focal charge compensation (FCC, Zeiss) and data was collected using a BSE detector (3View detector, Gatan). Imaging parameters for each stage of tactile bristle differentiation can be found in Supplementary Table 2 . The morphology of the neuronal dendrite from bristles lacking the F-Cell was visualized in the genotype neur>spi/EGFKD and no obvious defects were detected in the neuron and surrounding sheath cell membrane relative to wild-type controls.
3view detector
Manufactured by Ametek
The 3View detector is a core component of the Ametek lab equipment product line. It functions as a high-resolution imaging system designed for scientific and research applications. The device provides detailed three-dimensional visualization capabilities to aid in analysis and observation. Further details on its intended use are not available.
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Lab products found in correlation
2 protocols using 3view detector
1
Sputter Coating and 3View SEM Imaging
2
Ultrastructure Analysis of Tactile Bristle Differentiation
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Samples were sputter coated with a 10 nm layer of platinum (Q150S, Quorum Technologies) and loaded into a 3View2XP (Gatan) attached to a Sigma VP SEM (Zeiss) with focal charge compensation (FCC, Zeiss), and data were collected using a BSE detector (3View detector, Gatan). Imaging parameters for each stage of tactile bristle differentiation can be found in Supplementary Table 2 . The morphology of the neuronal dendrite from bristles lacking the F-Cell was visualized in the genotype neur > spi/EGFKD, and no obvious defects were detected in the neuron and surrounding sheath cell membrane relative to wild-type controls.
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