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Centro lb960 xs3

Manufactured by Berthold Technologies
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Sourced in Germany

The Centro LB960 XS3 is a compact multi-mode microplate reader designed for high-throughput screening and quantitative analysis. It features a patented monochromator-based optical system that provides flexible wavelength selection from 200 to 850 nm. The instrument supports a wide range of detection modes, including absorbance, fluorescence, and luminescence measurements.

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Lab products found in correlation

Psicheck 2, by GenePharma (2 mentions) Pmirglo vector, by Promega (2 mentions) Optical adhesive cover, by Thermo Fisher Scientific (1 mentions) Mir 154, by Panasonic (1 mentions) Nunclon delta, by Thermo Fisher Scientific (1 mentions) Mithras lb 940 multimode plate reader, by Berthold Technologies (1 mentions) Dual luciferase reporter assay kit, by Beyotime (1 mentions) Pmirglo, by Thermo Fisher Scientific (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Centro XS3 LB960 microplate LB960 Centro XS3 plate luminometer Centro XS3 LB960 High Sensitivity Microplate Luminometer Microplate Reader Centro XS3 LB960 Centro XS3 LB960 machine LB960

9 protocols using centro lb960 xs3

1

Dual-luciferase Assay for circRNA-miRNA Interactions

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Dual-luciferase reporter assay was used to evaluate the direct binding between circ-ZKSCAN1 and miRNAs. psiCHECK2 (GenePharma, China) vector contains firefly luciferase gene (hLuc+) and renilla luciferase gene (hRluc). The sequence of circ-ZKSCAN1 was cloned into the vector. NC vector or circ-ZKSCAN1 vector was co-transfected with each miRNAs mimics. The relative values of hLuc+ and hRluc were detected by Centro LB960 XS3 (Berthold, German). Luciferase reporter assay was used to detect whether p21 is the direct target of miR-1178-3p. The 3’UTR sequence of p21 was cloned into pcDNA3.0 vector. Next, miR-1178-3p mimics or NC was co-transfected with wild-type vector or the mutant vector. The relative value of luciferase was also detected by Centro LB960 XS3 (Berthold, German).
Bi J., Liu H., Dong W., Xie W., He Q., Cai Z., Huang J, & Lin T. (2019). Circular RNA circ-ZKSCAN1 inhibits bladder cancer progression through miR-1178-3p/p21 axis and acts as a prognostic factor of recurrence. Molecular Cancer, 18, 133.
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2

Binding Assay for circSPARC and JAK2

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A dual luciferase reporter assay was used to evaluate the direct binding between circSPARC and miRNAs as well as JAK2 and miRNAs. The circSPARC sequence was cloned into the psiCHECK-2 (GenePharma, China) vector, which contains the firefly luciferase gene (hLuc+) and Renilla luciferase gene (hRluc).. The NC vector or circSPARC vector was co-transfected with each miRNA mimic. The relative values of hLuc+ and hRluc were detected by Centro LB960 XS3 (Berthold, Germany). A luciferase reporter assay was used to determine whether JAK2 is the direct target of miR-485-3p. The 3’UTR sequence of JAK2 was cloned into the pcDNA3.0 vector. Next, the miR-485-3p mimic or NC was co-transfected with wild-type vector or the mutant vector. The relative luciferase value was also detected by Centro LB960 XS3 (Berthold, Germany).
Wang J., Zhang Y., Song H., Yin H., Jiang T., Xu Y., Liu L., Wang H., Gao H., Wang R, & Song J. (2021). The circular RNA circSPARC enhances the migration and proliferation of colorectal cancer by regulating the JAK/STAT pathway. Molecular Cancer, 20, 81.
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3

Dual-Luciferase Assay for circHERC4-miRNA Interactions

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We used a dual-luciferase reporter assay to confirm the direct binding between circHERC4 and miRNAs. PsiCHECK2 (IGE Biotech, China) vector included firefly luciferase gene (hLuc+) and renilla luciferase gene (hRluc). The sequence of circHERC4 was cloned into the psiCHECK2 vector. NC vector or circHERC4 vector was co-transfected with each miRNA mimics. The relative values of hLuc+ and hRluc were measured by Centro LB960 XS3 (Berthold, German). Luciferase reporter assay was applied to explore whether CTBP2 was the direct target of miR-556-5p. The 3’UTR sequence of CTBP2 was cloned into the pcDNA3.0 vector. Subsequently, CTBP2 vector was co-transfected with NC or miR-556-5p mimics. The relative value of luciferase was also evaluated by Centro LB960 XS3 (Berthold, German).
He J., Chu Z., Lai W., Lan Q., Zeng Y., Lu D., Jin S., Xu H., Su P., Yin D., Chu Z, & Liu L. (2021). Circular RNA circHERC4 as a novel oncogenic driver to promote tumor metastasis via the miR-556-5p/CTBP2/E-cadherin axis in colorectal cancer. Journal of Hematology & Oncology, 14, 194.
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4

Luciferase Developmental Rate Assay

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Luciferase developmental rate assays were performed as described (Olmedo et al., 2015 (link)). Briefly, single arrested L1 animals were transferred into one well of a 96-well plate containing 100 μl of S-basal medium with 200 μM D-Luciferin and simultaneously resumed development by adding 100 μl of S-basal with bacteria. Plates were sealed with a gas-permeable cover (Breathe Easier, Diversified Biotech) and luminescence was measured in a Berthold Centro LB960 XS3 for 1 sec, at 5-min intervals.
Zhang J., Li X., Olmedo M., Holdorf A.D., Shang Y., Artal-Sanz M., Yilmaz L.S, & Walhout A.J. (2019). A Delicate Balance Between Bacterial Iron and Reactive Oxygen Species Supports Optimal C. elegans Development. Cell host & microbe, 26(3), 400-411.e3.
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5

Bioluminescence Assay for Circadian Rhythms

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White, 96-well plates (Nunclon Delta, Thermo Fisher Scientific) were used, with each well inoculated with 5 × 105 cells. Plates were sealed with a transparent, evaporation-free cover (Optical Adhesive Covers, Applied Biosystems, Life Technologies). Cultures were exposed to light-darkness cycles as indicated in the text, after which the cultures were released to constant conditions (either constant darkness or light). The temperature was kept constant at 27°C. We measured bioluminescence (Berthold Centro LB960 XS3 or Berthold Mithras LB 940 Multimode Plate Reader) for 1 s each hour. All experiments were carried out in temperature-controlled incubators (MIR-154, Panasonic, Japan or Percival Intellus, Percival, USA). In entrainment experiments, the plates were ejected from the machine between readings for exposure to light.
Sartor F., Xu X., Popp T., Dodd A.N., Kovács Á.T, & Merrow M. (2023). The circadian clock of the bacterium B. subtilis evokes properties of complex, multicellular circadian systems. Science Advances, 9(31), eadh1308.
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6

Dual Luciferase Reporter Assay Protocol

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Dual luciferase reporter assay was performed using the procedure as previously described [23 (link)]. The relative values of hFluc and hRluc were detected by Centro LB960 XS3 (Berthold, German). Firefly luciferase activity was normalized to Renilla luciferase activity.
Liang M., Yao W., Shi B., Zhu X., Cai R., Yu Z., Guo W., Wang H., Dong Z., Lin M., Zhou X, & Zheng Y. (2021). Circular RNA hsa_circ_0110389 promotes gastric cancer progression through upregulating SORT1 via sponging miR-127-5p and miR-136-5p. Cell Death & Disease, 12(7), 639.
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7

Dual-Luciferase Assay for miRNA Binding

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Wild-type (wt) and mutant (mut) binding sequences of circ_0021350 or PIK3R3 3ʹUTR to miR-1207-3p were cloned into the pmirGLO vector (Promega, Madison, WI, USA). HEK-293T cells were seeded in 24-well plates at a density of 2 × 105cells/well and transfected with the corresponding plasmids and miR-1207-3p mimics/mimics-NC. Luciferase activity was measured following the manufacturer’s instructions using a dual-luciferase reporter assay kit (Beyotime, China) and detected using a Centro LB960 XS3 (Berthold, Germany).
Tan C., Wei J., Li Z., Tian N., Wang Z., Wang G., Han L, & Tian Y. (2023). Circ_0021350 plays an oncogene role by regulating miR-1207-3p/PIK3R3 in glioblastoma. BMC Cancer, 23, 808.
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8

Dual-Luciferase Assay for miR-17-5p and TLR4

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The binding site between miRNAs and target genes was examined by the use of dual-luciferase reporter assay as previously described [26 (link)]. Briefly, PsiCHECK2 (IGE Biotech, China) vector included firefly luciferase gene (hLuc+) and renilla luciferase gene (hRluc) was used in this assay. Luciferase reporter assay was performed to explore whether TLR4 was the direct target of miR-17–5p. The 3′ UTR sequence of TLR4 was cloned into the vectors (PsiCHECK2-WT-TLR4 and PsiCHECK2-MUT-TLR4). According to the manufacturer's instructions, TLR4 vector was co-transfected with NC or miR-17–5p mimics. The relative value of luciferase was measured by Centro LB960 XS3 (Berthold, German).
Chu Z., Huang Q., Ma K., Liu X., Zhang W., Cui S., Wei Q., Gao H., Hu W., Wang Z., Meng S., Tian L., Li H., Fu X, & Zhang C. (2023). Novel neutrophil extracellular trap-related mechanisms in diabetic wounds inspire a promising treatment strategy with hypoxia-challenged small extracellular vesicles. Bioactive Materials, 27, 257-270.
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9

Luciferase Assay for EGFR-3'UTR Binding

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The luciferase reporter assay was employed to evaluate the direct binding specificity between EGFR 3′UTR and IGF2BP3. For the luciferase reporter assays, a wild (EGFR–3’UTR) or mutant (EGFR–3′UTR-Mut #1 or #2) fragment was inserted into a pmirGLO vector (Promega, Madison, WI, USA) containing the firefly luciferase gene (hLuc+) and renilla luciferase gene (hRluc). UM cells cultured in 24-well plates at 60%–80% confluency were transfected with the pmirGLO wildtype or mutant reporter vector along with circ_0053943 plasmid or vectors using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol. The relative values of hLuc+ and hRluc were detected by Centro LB960 XS3 (Berthold, Bad Wildbad, German), and each measurement was repeated three times.
ZHAO A., WANG Y., WANG Z., SHAO Q., GONG Q., ZHU H., SHEN S., LIU H, & CHEN X. (2024). Circ_0053943 complexed with IGF2BP3 drives uveal melanoma progression via regulating N6-methyladenosine modification of Epidermal growth factor receptor. Oncology Research, 32(5), 983-998.
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