Dulbecco s modified eagle medium dmem

Dimethyl sulfoxide (DMSO) was purchased from Thermo-Fisher Scientific (USA). Both Dulbecco’s Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were obtained from Biowest (France). Tacrine was procured from Sigma-Aldrich Co. (St. Louis, MO, USA). Corn oil and carbon tetra chloride (CCl₄) were both obtained from Alpha Chemika (India). Carboxymethylcellulose sodium salt (CMC) as well as formalin were purchased from Adwic—El Nasr Pharmaceutical Co. (Egypt). Silymarin and isoflurane 100% were both acquired from Pharco-pharmaceuticals Inc. (Egypt). Phosphate buffered saline was obtained from Loba chemie (Mumbai, India).

N-Cetyltrimethylammonium bromide (CTAB), tetraethyl orthosilicate (TEOS), sodium hydroxide (NaOH), 3-aminopropyltriethoxysilane (APTES), pyruvic acid (PA), N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-(dimethylamino) propyl) carbodiimide hydrochloride (EDC), (2-thienylmethyl)hydrazine hydrochloride, (5,6-dimethylthieno[2,3-d]pyrimidin-4-yl)hydrazine, trifluoroacetic acid (TFA), triethylamine (TEA), ethanol, 2-propanol from Carl Roth (Carl Roth GmbH & Co. KG, Karlsruhe, Germany), dichloro(p-cymene)ruthenium(II) dimer, phosphate buffered saline (PBS), toluene and crystal violet (CV) were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Dulbecco’s Modified Eagle Medium (DMEM) was purchased from Biowest (Riverside, CA, USA). For in vitro experiments nutrition medium RPMI 1640, FCS (fetal calb serum), penicillin/streptomycin and phosphate buffer saline (PBS) were obtained from Capricorn scientific GmbH (Ebsdorfergrund, Germany), glutamine and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) from GE healthcare (Chicago, IL, USA) and Biomol GmbH (Hamburg, Germany), respectively.

Singlet Oxygen Sensor Green was purchased from Thermo Fisher Scientific. Dulbecco’s Modified Eagle Medium (DMEM) and foetal bovine serum (FBS) were from Biowest. All the other reagents were from Sigma‐Aldrich, St. Louis, MO, USA.

To perform cell viability assays, we used dermal fibroblasts isolated from rat embryos by enzymatic tissue dissociation [32 (link)]. After isolation and fragmentation of skin, incubation with 0.25% trypsin-EDTA purchased from BioWest (France) for 1 h at 37°С was performed with a magnetic stirrer. After addition of 10% fetal bovine serum (FBS, Lonza, Germany), the cells were washed in Dulbecco's modified eagle medium (DMEM) purchased from BioWest (France). The cells were seeded in 25 cm² SPL culture flasks (Republic of Korea). When the monolayer confluence reached 100%, the cells were harvested by 0.25% trypsin-EDTA. The fibroblasts used in the experiment underwent 3-4 passages.
The fibroblasts were incubated with a polymer (panel a), hybrid Ag-polymer composite (panel b), and hybrid Au-polymer composite (panel c) at concentrations of 0-0.5-1-2-5-10-20-30-50 mg/L for 24 h to assess the monolayers visually and perform cell viability tests.
Images of cell cultures were acquired using an inverted phase contrast microscope NIB-100 (Delta Optical, Poland) equipped with a UCMOS 3100 camera (SIGETA, Hangzhou, PRC). ToupView v.3.7 (Hangzhou Toup Tek Photonics Co. Ltd, Hangzhou, PRC) and ImageJ V.1.48. (National Institute of Health, USA) software were used.

Silkworm (B. mori) pupae were obtained from Insect Vision (Yangju, Republic of Korea) and stored in a deep freezer (−50 °C) to preserve the specimens until use. Liquid chromatography (LC)-grade water was purchased from Honeywell (Charlotte, NC, USA). Guaranteed reagent (GR)-grade methanol (MeOH) and extra pure (EP)-grade hexane were purchased from Daejung Chemicals & Metals (Daejung, Siheung, Republic of Korea). Mass spectrometry (MS)-grade water, acetonitrile, and formic acid were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Dulbecco’s phosphate-buffered saline (DPBS) and Dulbecco’s modified Eagle medium (DMEM) were purchased from Biowest (Cholet, France), and PBS was purchased from Bio-Rad Laboratories (Hercules, CA, USA). Horse serum, penicillin–streptomycin, and trypsin were purchased from Gibco (Grand Island, NY, USA). L-Isoleucine and L-Valine were purchased from Sigma-Aldrich (St. Louis, MO, USA).

NSC34 cells were cultured in a 10 % FBS, 1 % PS and high glucose Dulbecco’s modified eagle medium (DMEM; Biowest, Nuaille, France). To express MPV17 transcript in the motor neuron, NSC34 cells were transfected with MPV17 DNA-containing vectors using Lipofectamine 2000 reagent (Invitrogen), according to the manufacturer’s recommendation. Knockdown of MPV17 was performed using MPV17-specific siRNA and Lipofectamine 2000 reagent (Invitrogen) (Additional file 1: Table S1). Cells were harvested after overexpression and knockdown of MPV17 at 24 and 72 h.

SuperFrost Plus slides were purchased from Gerhard Menzel GmbH (Braunschweig, Germany). Target retrieval solution (TRS), Tris-buffered saline (TBS), EnVision+System-HRP, and 3,3′-diaminobenzidine were obtained from Dako Corp. (Carpinteria, USA). Dulbecco’s modified eagle medium (DMEM) was purchased from Biowest (Kansas City, USA). Hank’s balanced salt solution (HBSS), sodium bicarbonate, fetal calf serum (FCS), gentamicin, and glutamine were obtained from GIBCO (Gaithersburg, USA). Hydrogen peroxide solution, NaCl, DPX mountant for histology, Percoll®, hematoxylin, toluidine blue, trypan blue, and phosphate-buffered saline (PBS) were purchased from Sigma-Aldrich (St. Louis, USA). Recombinant rat leptin and normal rabbit IgG antibodies were obtained from R&D Systems (Minneapolis, USA). BD CellFIX™ was obtained from BD Biosciences (Benelux, Belgium). Rabbit polyclonal IgG antibodies against Ob-R were purchased from Thermo Fischer Scientific (Waltham, USA). Alexa Fluor® 488-conjugated goat anti-rabbit polyclonal antibodies were obtained from Jackson ImmunoResearch Laboratories, Inc. (West Grove, USA).