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Mabn310

Manufactured by Merck Group

MABN310 is a laboratory equipment product from Merck Group. It is designed for use in various scientific applications. The core function of MABN310 is to perform specific tasks in a controlled laboratory environment.

Automatically generated - may contain errors

2 protocols using mabn310

1

Western Blot Analysis of GPR56 in Hematopoietic and Neural Tissues

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Total protein lysates from the FACS-sorted BM HSPCs, liver and embryonic brain (embryonic day (E) 14.5) were subjected to standard western blot analysis. Total protein was loaded onto 4–16% gradient SDS-PAGE gel and transferred onto a PVDF membrane. Mouse anti-human GPR56 monoclonal antibody (1:500 dilution, Millipore catalog #MABN310, (27 (link))) was used to detect GPR56 protein. β-actin (Santa Cruz) used as loading control.
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2

Immunofluorescence Staining of Tissue Sections

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Frozen and/or paraffin sections were used for immunofluorescence staining as described previously 10 (link)13 (link) using the following primary antibodies: WT-1 (sc-192Santa Cruz Biotechnology), CD31 (BDB550274, BD Biosciences), cleaved PARP (#ab32064, Abcam), cleaved Caspase-3 (#9664, Abcam), 8-oxoG monoclonal antibody (N45.1; Japan Institute for the Control of Aging), LRG1 (13224-1-AP, Proteintech), GPR56 (MABN310, Millipore), and ZFP57 (ab45341, Abcam). Isolectin GS-IB4 (121413, Invitrogen) was also used to detect endothelial cells. Fluorescence images were acquired using the Axioplan 2 IE microscope (Zeiss).
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