V plex aβ peptide panel 1

Manufactured by MSD

Sourced in United States

The V-PLEX Aβ peptide Panel 1 is a laboratory equipment product designed for the quantitative measurement of amyloid-beta (Aβ) peptides. It utilizes electrochemiluminescence technology to detect and quantify different Aβ isoforms, including Aβ40 and Aβ42.

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Lab products found in correlation

At270, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

V-PLEX Aβ Peptide Panel 1 (4G8) (2 citations) V-PLEX Aβ Peptide Panel 1 (6E10) (2 citations) V-PLEX Plus Aβ Peptide Panel 1 Kit (2 citations) V-PLEX Plus Aβ Peptide Panel 1 (6E10) Kit (2 citations) V-PLEX Aβ Peptide Panel 1 (6E10) Kit (2 citations)

2 protocols using v plex aβ peptide panel 1

Plasma Aβ Levels in TgF344-AD Rats

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Blood samples (200–250 μl) were taken from the tail veins of F344 (and TgF344-AD rats. Plasma samples (100 μl) were prepared using 10% potassium K-EDTA mixed with whole blood at final 0.1% K-EDTA dilution. Blood sample were centrifuged at 1500g to extract supernatant plasma. Prepared plasma samples were stored at -80 °C for subsequent biochemical analysis with ELISA. A Meso Scale Discovery (MSD) platform was used to test the samples. Assays for Aβ 40 and 42 were analyzed using V-PLEX Aβ peptide Panel 1, following the manufacturer's instructions (MSD, Gaithersburg, Maryland, USA). An analysis of the variance with Kruskal-Wallis test for plasma concentrations of Aβ42 and Aβ40 in WT F344 and TgF344-AD revealed no significant differences due to sex. Data from males and females was therefore merged prior to analysis for effects of genotype on Aβ42 and Aβ40 (Table S4).

Ratner M.H., Downing S.S., Guo O., Odamah K.E., Stewart T.M., Kumaresan V., Robitsek R.J., Xia W, & Farb D.H. (2021). Prodromal dysfunction of α5GABA-A receptor modulated hippocampal ripples occurs prior to neurodegeneration in the TgF344-AD rat model of Alzheimer's disease. Heliyon, 7(9), e07895.

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Validating TMT-based Proteomic Profiling

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To validate findings from the TMT-based proteomic profiling using an independent method, a Meso Scale Discovery (MSD) platform was used to test the same material for MS analysis. Assays for Aβ, tau, IL-6, and SAA were analyzed using V-PLEX Aβ peptide Panel 1, total Tau kit, Neuroinflammation Panel 1 Human Kit, following the manufacturer’ instructions (MSD, Gaithersburg, MD). Antibodies AT270 and HT-7 (Thermo Fisher Scientific, Waltham, MA) were used to capture/detect pTau181 using the same MSD platform.

Chen M, & Xia W. (2020). Proteomic Profiling of Plasma and Brain Tissue from Alzheimer’s Disease Patients Reveals Candidate Network of Plasma Biomarkers. Journal of Alzheimer's disease : JAD, 76(1), 349-368.

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