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L α lysophosphatidylcholine

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L-α-lysophosphatidylcholine is a lipid molecule used in various laboratory applications. It is a type of phospholipid that can be utilized as a reagent or component in experimental procedures. The core function of this product is to serve as a laboratory tool, without further interpretation of its intended use.

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Lab products found in correlation

Thapsigargin, by Merck Group (1 mentions) Skf96365, by Merck Group (1 mentions) Fitc labeled phalloidin, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Cxcl12, by Thermo Fisher Scientific (1 mentions) Cholesterol, by Merck Group (1 mentions) Cholesteryl oleate, by Merck Group (1 mentions) Glycerol trioleate, by Merck Group (1 mentions) Egg yolk phosphatidylcholine, by Lipoid (1 mentions) Thioflavin t tht dye, by Merck Group (1 mentions) Tamoxifen, by Merck Group (1 mentions) Mnase, by Thermo Fisher Scientific (1 mentions) Infinite m200 pro, by Tecan (1 mentions) L α phosphatidylcholine, by Merck Group (1 mentions) L α phosphatidylinositol, by Merck Group (1 mentions) L α phosphatidylethanolamine, by Merck Group (1 mentions) Soybean phosphatidylcholine, by Avanti Polar Lipids (1 mentions)

Spelling variants of this product

Lysophosphatidylcholine (26 citations) L-α-Lysophosphatidylcholine (lysolecithin; (3 citations) L-α- lysophosphatidylcholine; Sigma L4129 (2 citations)

21 protocols using l α lysophosphatidylcholine

1

Cholesterol and Calcium Signaling

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Water-soluble cholesterol, SKF-96365 (SKF), Thapsigargin (Tg), l-α-Lysophosphatidylcholine (LPC) were all purchased from Sigma.
Andrews A.M., Muzorewa T.T., Zaccheo K.A., Buerk D.G., Jaron D, & Barbee K.A. (2016). Cholesterol Enrichment Impairs Capacitative Calcium Entry, eNOS Phosphorylation & Shear Stress-Induced NO Production. Cellular and Molecular Bioengineering, 10(1), 30-40.
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2

Focal Demyelination in Mouse Corpus Callosum

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After being anesthetized with a mixture of Ketamine (200 mg/kg) and Xylazine (15mg/kg) (Sigma-Aldrich, Cat# K4138), mice were fixed on a stereotaxic instrument (Stoelting). One μl of 1% L-α-Lysophosphatidylcholine (LPC or lysolecithin in physiological saline; Sigma-Aldrich, Cat# L4129) was injected into right CC (AP: +1.0 mm; ML: −0.8 mm; DV: 2.1 mm from bregma) with Hamilton syringe. The Hamilton syringe was equipped with a 33-gauge needle (45° beveled tip) attached to a motorized stereotaxic injector (Stoelting), and LPC was injected at a rate of 0.05 μl/min. After the injection, the needle was held in place for an additional 10 min and gently pulled out. The scalp was sutured, and the mice were allowed to recover from anesthesia.
Khawaja R.R., Agarwal A., Fukaya M., Jeong H.K., Gross S., Gonzalez-Fernandez E., Soboloff J., Bergles D.E, & Kang S.H. (2021). GluA2 overexpression in oligodendrocyte progenitors promotes postinjury oligodendrocyte regeneration. Cell reports, 35(7), 109147.
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3

Chemically Induced Demyelination in Mice

Cited 2 times
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Demyelinated lesions were chemically induced by focal injection of lysolecithin into the spinal cord ventrolateral white matter of 8- to 12-wk-old C57Bl/6 mice as previously described (Mei et al., 2014 (link)) with modifications highlighted in the following experimental description. Animals were anesthetized via intraperitoneal injection of ketamine (100 mg/kg) and xylazine (15 mg/kg). A midline skin incision was made over the upper lumbar regions of spinal cord, and the spinal column was secured with mouse vertebral clamps fixed in a stereotaxic frame. The epidural space was exposed by disruption of the L1-L2 interspinous ligament without laminectomy as described (Ryu et al., 2015 (link)). A pulled-glass micropipette secured to a Hamilton syringe was prefilled with 1% lysolecithin (L-α-lysophosphatidylcholine, Sigma-Aldrich) and inserted into the spinal cord 0.3 mm lateral to the spinal midline and a depth of 0.9 mm from the spinal cord surface. Then, 1 μL of 1% lysolecithin was injected (0.2 μl/min) and the glass micropipette remained in place for 5 min before being slowly withdrawn. After surgery, skin was sutured, and mice were allowed to recover.
Petersen M.A., Ryu J.K., Chang K.J., Etxeberria A., Bardehle S., Mendiola A.S., Kamau-Devers W., Fancy S.P., Thor A., Bushong E.A., Baeza-Raja B., Syme C.A., Wu M.D., Rios Coronado P.E., Meyer-Franke A., Yahn S., Pous L., Lee J.K., Schachtrup C., Lassmann H., Huang E.J., Han M.H., Absinta M., Reich D.S., Ellisman M.H., Rowitch D.H., Chan J.R, & Akassoglou K. (2017). Fibrinogen activates BMP signaling in oligodendrocyte progenitor cells and inhibits remyelination after vascular damage. Neuron, 96(5), 1003-1012.e7.
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4

PBMC F-actin Polymerization Assay

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Isolated PBMCs were cultured for 12 hours in R-10 before the F-actin polymerization test. The cells were first stained with PE-conjugated anti-CD45R/B220 mAb to discriminate between B- and T-cell populations before being subjected to the F-actin polymerization test. Intracellular F-actin polymerization was assessed as previously described [31 (link)]. Briefly, the cells were harvested and resuspended (4 x 106/ml) in HEPES-buffered RPMI-1640 at 37°C with or without CXCL12 (500 ng/ml). At the indicated times, the cell suspensions (100 μl) were added to 400 μl of assay buffer containing 4 x 107 FITC-labeled phalloidin, 0.5 mg/ml L-α-lysophosphatidylcholine (both from Sigma-Aldrich) and 4.5% formaldehyde in PBS. The fixed cells were analyzed by flow cytometry, and the mean fluorescence intensity (MFI) was determined for each sample. The percent change in MFI was calculated for each sample at each time point according to the following formula: (1-(MFI before the addition of CXCL12/MFI after the addition of CXCL12)) × 100.
Badr G., Sayed A., Abdel-Maksoud M.A., Mohamed A.O., El-Amir A., Abdel-Ghaffar F.A., Al-Quraishy S, & Mahmoud M.H. (2015). Infection of Female BWF1 Lupus Mice with Malaria Parasite Attenuates B Cell Autoreactivity by Modulating the CXCL12/CXCR4 Axis and Its Downstream Signals PI3K/AKT, NFκB and ERK. PLoS ONE, 10(4), e0125340.
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5

Actin Cytoskeleton Dynamics in MCL Cells

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MCL cells (107 cells/mL) were washed twice and serum starved for 1.5 hours in FBS-free RPMI-1640. Acadesine was added for 3 additional hours, and MCL primary cells were diluted to 2 × 106 cells/mL in RPMI-1640 with 0.5% bovine serum albumin (BSA; Sigma). Thereafter, samples were stimulated with 200 ng/mL of CXCL12 (Peprotech) and at the indicated time points, 100 μL of the cell suspension was collected and added to 25 μL of the staining solution [2.5 ng/mL phalloidin-Tetramethyl Rhodamine Isothiocyanate (TRITC), 2.5 mg/mL of L-α-lysophosphatidylcholine (Sigma) and 5% paraformaldehyde (Aname)] for 20 minutes at 37°C. Cells were acquired on an Attune cytometer, and results were plotted relative to the mean fluorescence of the sample before the addition of CXCL12.
Montraveta A., Xargay-Torrent S., Rosich L., López-Guerra M., Roldán J., Rodríguez V., Lee-Vergés E., de Frías M., Campàs C., Campo E., Roué G, & Colomer D. (2015). Bcl-2high mantle cell lymphoma cells are sensitized to acadesine with ABT-199. Oncotarget, 6(25), 21159-21172.
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6

Lipid Tracer Synthesis and Characterization

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Glycerol trioleate (triolein; TO; ≥99%), L‐α‐lysophosphatidylcholine (≥99%), cholesteryl oleate (CO; ≥98%), and cholesterol (≥99%) were purchased from Sigma‐Aldrich. Egg yolk phosphatidylcholine (98%) was obtained from Lipoid (Switzerland). [3H]TO and [14C]CO were obtained from PerkinElmer.
Ying Z., Boon M.R., Coskun T., Kooijman S, & Rensen P.C. (2021). A simplified procedure to trace triglyceride‐rich lipoprotein metabolism in vivo. Physiological Reports, 9(8), e14820.
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7

Preparation and Characterization of Human IAPP Amyloids

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Human islet amyloid polypeptide (IAPP; 37 residues, 2–7 disulfide bridge, 3.9 kDa, >95% pure by HPLC) was obtained in lyophilised monomeric form from AnaSpec, and prepared in Milli-Q water at a stock concentration of 200 μM at room temperature with mixing immediately prior to use. Mature IAPP amyloids were aqueous solutions of IAPP incubated for more than 24 h. Thioflavin T (ThT) dye and L-α-Lysophosphatidylcholine (LPC; from Glycine max, >99% pure by TLC) were purchased from Sigma-Aldrich. LPC derived from soybean is primarily composed of unsaturated C-18 fatty acids; typically 40–60% linoleic, 25–30% palmitic, 10–12% oleic, 7–10% stearic and 4–6% linolenic acid.
Xing Y., Pilkington E.H., Wang M., Nowell C.J., Kakinen A., Sun Y., Wang B., Davis T.P., Ding F, & Ke P.C. (2017). Lysophosphatidylcholine modulates the aggregation of human islet amyloid polypeptide. Physical chemistry chemical physics : PCCP, 19(45), 30627-30635.
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8

Lysolecithin-Induced Demyelination in miR-219 Mice

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6- to 8-week-old miR-219-1lox/lox, miR-219-2lox/lox, Rosa2tdTomato, Plp-CreERt mice and miR-219-1lox/+, miR-219-2lox/+, Rosa2tdTomato, Plp-CreERt control mice, wild type and miR-219 transgenic mice were used in the lysolecithin-induced demyelination experiments. Anesthesia was induced and maintained by i.p. injection of a mixture of ketamine (90 mg/kg) and xylazine (10 mg/kg). After exposing the spinal vertebrae at the level of T9-T12, meningeal tissue in the intervertebral space was cleared, and the dura was pierced with a dental needle. 0.5 μl of 1 % lysolecithin (l-α-lysophosphatidylcholine, Sigma L4129) via a Hamilton syringe attached a glass micropipette was injected into the ventrolateral white matter using a stereotactic apparatus. LPC-induced lesions in brain were stereotactically performed to the genu of corpus callosum. Injuries were conducted in a genotype-blinded manner. For tamoxifen treatment of Plp-CreERt-miR-219-1/2lox/lox mice, tamoxifen (Sigma T5648) was dissolved in corn oil (Sigma, C-8267) and injected intraperitoneally at 50 mg/kg body weight (Sigma). Tissues carrying the lesions were collected at different time points.
Wang H., Moyano A.L., Ma Z., Deng Y., Lin Y., Zhao C., Zhang L., Jiang M., He X., Ma Z., Lu F., Xin M., Zhou W., Yoon S.O., Bongarzone E.R, & Lu Q.R. (2017). miR-219 Cooperates with miR-338 in Myelination and Promotes Myelin Repair in the CNS. Developmental cell, 40(6), 566-582.e5.
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9

Lysolecithin-Induced Prefrontal Cortex Lesion

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While under deep anesthesia induced by inhaled isoflurane, experimental C57BL/6J mice were surgically injected with 1 μl 1% lysolecithin (l-α-lysophosphatidylcholine, Sigma-Aldrich) dissolved in saline, or saline as sham control, bilaterally to the medial prefrontal cortex using a pulled capillary glass pipet at the following stereotaxic coordinates: anterioposterior,+1.5 mm; mediolateral from bregma, 0.5 mm; and dorsoventral-below the surface of the dura, 1.5 mm. The needle was left in place for an additional 2 min to avoid back flow of the lysolecithin or saline. Muscle and skin incisions were sutured with gut and nylon sutures, respectively. To reduce postoperative pain after recovery from anesthesia, animals received a subcutaneous injection of buprenorphine (1.0 mg/kg). Animals were monitored closely following surgery and were tested with social interaction tests at 7- and 21 days post injection.
Bonnefil V., Dietz K., Amatruda M., Wentling M., Aubry A.V., Dupree J.L., Temple G., Park H.J., Burghardt N.S., Casaccia P, & Liu J. (2019). Region-specific myelin differences define behavioral consequences of chronic social defeat stress in mice. eLife, 8, e40855.
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10

Demyelination Induction in Mouse Spinal Cord

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Demyelination in mice was induced by injection of 1 µL of 1% L-α-lysophosphatidylcholine (lysolecithin, SIGMA, St. Louis, MI, USA) into the ventral and dorsal white matter funiculi at the level of T12 as previously described in detail [40 (link),41 (link)]. Briefly, the position of T13 was identified and the epaxial musculature was cleared from the immediate area. The space between T12 and T13 was exposed and carefully cleared, the central vein was identified, and the dura was pierced with a dental needle lateral to the vein. A three-way manipulator was then used to position the needle for stereotaxic injection of EB. Hamilton needle with a fine glass tip was advanced through the pierced dura at an angle appropriate for ventrolateral or dorsal funiculus injection. The injection was controlled at 1 µL per minute and the needle remained in the injection site for 2 min to allow maximal diffusion of toxin.
During all surgical procedures, animals were anesthetized with isoflurane supplemented with buprenorphine (0.03 mg/kg, intraperitoneal injection) for pain relief.
Wylot B., Mieczkowski J., Niedziolka S., Kaminska B, & Zawadzka M. (2019). Csf1 Deficiency Dysregulates Glial Responses to Demyelination and Disturbs CNS White Matter Remyelination. Cells, 9(1), 99.
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