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5 protocols using pb ch3coo 2

1

Graphite-based Nanocomposite Synthesis

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Graphite powder (<50
μm), NaNO3, H2SO4 (98%), KMnO4, HCl (37%), H2O2 (30%), 3,5-diaminobenzoic
acid (DABA), AlCl3, Pb(CH3COO)2,
NH4OH, ethanol, methanol, and acetone were purchased from
Sigma-Aldrich.
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2

Fabrication of Iodide Perovskite Films

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For the iodide perovskite films, 3:1 molar stoichiometric ratios of CH3NH3I and Pb(CH3COO)2 (Sigma Aldrich 99.999% pure) were made in N,N-dimethylformamide in 20 wt% solution. This solution was spun inside a nitrogen filled glove box on quartz substrates at 2000 r.p.m. for 60 s followed by 3 min of thermal annealing at 100 °C in air to form thin films. The samples were encapsulated with a second glass slide and epoxy adhesive (Loctite Double Bubble) under inert conditions to avoid sample degradation and beam damage.
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3

Synthesis of Metal Nanoparticles

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Gold(III) chloride trihydrate (HAuCl4·3H2O), cetyltrimethylammonium bromide (CTAB), silver nitrate (AgNO3) and l-ascorbic acid (AA) were all obtained from Sigma-Aldrich. Sodium borohydride (NaBH4) and sodium hydroxide (NaOH) were purchased from SAMCHUN chemical. CrO3, AgNO3, Ca(CH3COO)2, Cd(CH3COO)2, AlCl3, BaCl2, CoCl2, FeSO4, MnCl2, NaOH, Pb(CH3COO)2, LiCH3COO, Mg[CH3COCHC(O)CH3]2 were bought from Sigma-Aldrich. Hg(CH3COO)2, FeSO4, ZnCl2, KCl, NaOH, Ni(NO3)2 were procured from SAMCHUN chemical. Ultrapure deionized water was used to prepare all solutions.
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4

Perovskite Thin Film Fabrication

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For the iodide and bromide perovskite films: 3:1 molar stoichiometric ratios of CH3NH3I and CH3NH3Br for bromide and Pb(CH3COO)2 (Sigma Aldrich 99.999% pure) were made in N,N-dimethylformamide (DMF) in 20wt% solution. These solutions were spun inside a nitrogen filled glove box on quartz substrates at 2,000 r.p.m. for 60 s followed by 3 min of thermal annealing at 100 °C in air to form thin films. For iodide–chloride perovskite, PbCl2 was used in the precursor instead of Pb(CH3COO)2.
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5

G-quadruplex Probe for Pb2+ Detection

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The G-quadruplex probe for Pb2+ detection was 5′-GGGTGGGTGGGTGGGT-3′ [38 (link)]. It was chemically labeled with a FAM at its 5′-terminal, which was HPLC purified. Tris-HCl and labeled aptamer were obtained from Shanghai Sangon Biological Engineering Technology & Services Co., Ltd. (Shanghai, China). Pb (CH3COO)2 was purchased from Sigma Aldrich (Mississauga, ON, Canada). Mn (CH3COO)2 was purchased from Tianjin Damao Chemical Reagent Factory (Tianjin, China), CoCl2, Cd (NO3)2, CuSO4, Al (NO3)3, KCl, NiCl2, MgCl2, HNO3, and HClO4 were purchased from Chengdu Kelon Chemical Reagent Factory (Chengdu, China). HgCl2 was purchased from Tongren Chemical Industry Research Institute (Tongren, China). NMM was provided by Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Molecular biology-grade water was provided by Corning (Corning, NY, USA) and used for solution preparation.
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