Personal genome machine (pgm)

Manufactured by PromoCell

Sourced in Germany

The PGM is a laboratory equipment that performs the function of measuring and analyzing the pH, conductivity, and oxidation-reduction potential of samples. It is a versatile device designed for use in various scientific and industrial applications that require precise monitoring and measurement of these parameters.

Automatically generated - may contain errors

Lab products found in correlation

Fgm 2, by Lonza (1 mentions) Hpc pl, by PromoCell (1 mentions) Egm 2, by Lonza (1 mentions) Huvecs, by Lonza (1 mentions)

2 protocols using personal genome machine (pgm)

Culturing Primary Human Vascular Cells

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Human umbilical vein endothelial cells (HUVECs, Lonza, Switzerland) were cultured in endothelial growth medium (EGM-2, Lonza, Switzerland) with full supplements and were used at passage 4. Dermal fibroblasts (DFs, CEFO, Korea) and normal human lung fibroblasts (LFs, Lonza, Switzerland) were grown in fibroblast growth medium (FGM-2, Lonza, Switzerland) with full supplements and were used at passage 6–8. Human placental pericytes (hPC-PL, Promocell, Germany) were cultured in pericyte growth medium (PGM, Promocell, Germany) and used at passage 6–8. All cells were cultured in a humidified 5% CO₂ incubator at 37°C.

Kim J., Chung M., Kim S., Jo D.H., Kim J.H, & Jeon N.L. (2015). Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network. PLoS ONE, 10(7), e0133880.

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Isolation of hASCs and Pericytes

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Human adipose-derived stem cells (hASCs) and pericytes were isolated from human tissue derived from patients that underwent regular surgical treatment and signed an informed content at the BG University Hospital Bergmannsheil in Bochum, Germany. The hASCs were cultured in DMEM-HAMS-F12, and the pericytes were cultured in a pericytes-growth-medium (PGM, PromoCell).

Han X., Courseaus J., Khamassi J., Nottrodt N., Engelhardt S., Jacobsen F., Bierwisch C., Meyer W., Walter T., Weisser J., Jaeger R., Bibb R, & Harris R. (2018). Optimized vascular network by stereolithography for tissue engineered skin. International Journal of Bioprinting, 4(2), 134.

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