Anti ulbp2

Manufactured by R&D Systems

Sourced in United States

Anti-ULBP2 is a recombinant protein that binds to the ULBP2 receptor. ULBP2 is a member of the UL16-binding protein (ULBP) family, which are ligands for the NKG2D receptor expressed on natural killer cells and some T cells. The binding of Anti-ULBP2 to ULBP2 may be used to study NKG2D-mediated immune responses.

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Lab products found in correlation

Anti ulbp1, by R&D Systems (3 mentions) Anti ulbp3, by R&D Systems (2 mentions) Anti cd56, by BD (1 mentions) Anti cd3, by BioLegend (1 mentions) Anti nkg2d antibody, by R&D Systems (1 mentions) Anti cd107a lamp 1, by BioLegend (1 mentions) Anti mica b, by R&D Systems (1 mentions) Anti vinculin, by Merck Group (1 mentions) Anti mica, by R&D Systems (1 mentions) Anti mouse igg antibody, by Jackson ImmunoResearch (1 mentions) Anti micb, by R&D Systems (1 mentions) Alexa fluor 647, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

ULBP2 (5 citations) Anti-ULBP2/5/6-PE (4 citations) PE-conjugated anti-human ULBP2 (2 citations)

4 protocols using anti ulbp2

Quantification of ULBP Proteins by ELISA and Flow Cytometry

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Anti-ULBP1, anti-ULBP2 and anti-ULBP3 antibodies were purchased from R&D systems (catalog numbers: MAB1380, 1248 and 1517 respectively) and used both for flow cytometry and for ELISA assays. Anti-ULBP1 antibody, (catalog number Sc33564, Santa Cruz Biotechnology) was used for western blotting of ULBP1. The W6/32 mAb was used for MHC-I staining. Anti-NKG2D antibody was purchased from R&D Systems (MAB139). The anti-CD99 (12E7) was used as an isotype control. Anti-CD107a (LAMP-1) was purchased from BioLegend (catalog number 328620). Anti-CD56 (Becton Dickinson) and anti-CD3 (BioLegend) antibodies were used to determine NK purity. Anti-VP2/3 and agnoprotein antibodies were produced in house as well as the rabbit polyclonal antibodies against VP1. Anti-T-Ag antibody was purchased from Abcam (Pab416). The commercial recombinant ULBP-1 Fc chimeric protein (R&D systems, catalog number 1380-UL) was used for the generation of ULBP1 standard curve.
CD16-Ig, NKp30-Ig and NKp46-Ig and NKG2D-Ig fusion proteins were generated in the human embryonic kidney 293T cells and were purified on a protein G column as described [38 (link)]. The fusion proteins used in this work were regularly assayed by SDS-PAGE protein gels, to ensure that the proteins were not degraded. Protein purity of all Ig fusion proteins used in this study was approximately 100%.

Bauman Y., Drayman N., Ben-Nun-Shaul O., Vitenstein A., Yamin R., Ophir Y., Oppenheim A, & Mandelboim O. (2016). Downregulation of the stress-induced ligand ULBP1 following SV40 infection confers viral evasion from NK cell cytotoxicity. Oncotarget, 7(13), 15369-15381.

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Evaluation of Tumor-derived Soluble NKG2DL Inhibition

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T cells were resuspended in complete medium with a density of 1×10⁶ cells/ml and were cultured with anti-CD28 (2 µg/ml) and IL-2 (10 U/ml) in a 24-well plate pre-coated with 5 µg/ml anti-CD3. Subsequently, T cells were co-cultured with or without 100 µl serial diluted tumor sup for 24 h. For neutralizing sNKG2DL, single and combined soluble NKG2DL neutralizing antibodies (1, 2, or 5 µg/ml anti-MICA/B, ULBP1, ULBP2, and/or ULBP3) were added to T cells and to the tumor cell supernatant co-culture system. Then, these T cells were harvested and prepared for flow cytometric analysis. Neutralized antibodies (anti-MICA/B, anti-ULBP1, anti-ULBP2, and anti-ULBP3) were purchased from R&D Systems, Inc. (cat. nos. MAB13001, MAB1380, MAB1298 and MAB1517, respectively).

Zhang Y., Luo F, & Dong K. (2023). Soluble NKG2D ligands impair CD8+ T cell antitumor function dependent of NKG2D downregulation in neuroblastoma. Oncology Letters, 26(1), 297.

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Analyzing Tumor Protein Fractions

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Protein fractions were extracted from tumors grown in athymic mice and analyzed by Western blotting as described [39 (link)]. Antibodies used were: anti-vinculin (Sigma-Aldrich, St. Louis, MO, USA), anti-MICA and anti-ULBP2 (R&D Systems, Minneapolis, MN, USA).

Di Modica M., Sfondrini L., Regondi V., Varchetta S., Oliviero B., Mariani G., Bianchi G.V., Generali D., Balsari A., Triulzi T, & Tagliabue E. (2015). Taxanes enhance trastuzumab-mediated ADCC on tumor cells through NKG2D-mediated NK cell recognition. Oncotarget, 7(1), 255-265.

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Flow Cytometric Evaluation of Stress-Induced Ligands

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Cells were stained with a mouse isotype IgG2b antibody (Biolegend), with anti-MICA, anti-MICB, anti-ULBP1, anti-ULBP2 (the antibody also recognizes ULBP5 and ULBP6), anti-ULBP3 antibodies (all obtained from R&D Systems), or with the anti-HLA-A, B, C monoclonal antibody W6/32 antibody (purified from the hybridoma). 0.2 µg primary antibodies per staining were added in 100 µL PBS (2 µg/mL) supplemented with 1% BSA and 1mM EDTA and incubated for about 30 minutes at 4°C. Following one wash, the primary antibody was detected using an anti-mouse IgG antibody coupled to Alexa Fluor 647 (Jackson ImmunoResearch) for another 30 minutes at 4°C. For the characterization of cells overexpressing viral genes, staining of the stress-induced ligands was assessed on GFP positive cells.

Chaouat A.E., Seliger B., Mandelboim O, & Schmiedel D. (2021). The HHV-6A Proteins U20 and U21 Target NKG2D Ligands to Escape Immune Recognition. Frontiers in Immunology, 12, 714799.

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