Forty-eight hours after transfection, 1 × 104 of 0.05% trypsin-EDTA (HyClone, Cytiva)-detached cells in a final volume of 100 µL were seeded in Costar ultra-low attachment 96-well round-bottom plates (Cat No. 7007, Corning, Inc.) in complete medium (12 replicates). Spheroid formation was monitored and visualized using AxioObserver D1 microscope (10× lens; Carl Zeiss AG) and AxioVision LE software (Carl Zeiss AG) as previously described [32 (link)]. The area of spheroids was calculated using ImageJ (NIH) software. The results are presented as the percentage of spheroid area compared to controls (siNEG-treated cells).
Axiovision le
Manufactured by Zeiss
Sourced in Germany
AxioVision LE is a microscope imaging software developed by Zeiss. It provides basic image acquisition, processing, and analysis capabilities for various microscope systems.
Automatically generated - may contain errors
Lab products found in correlation
Axiocam, by Zeiss (3 mentions)
Axio vert a1 inverted microscope, by Zeiss (3 mentions)
Axiocam digital camera system, by Zeiss (3 mentions)
Cm1850, by Leica (2 mentions)
Primo star, by Zeiss (2 mentions)
Prism 3, by GraphPad (2 mentions)
Stemi 2000 c, by Zeiss (2 mentions)
Axiocam mrm, by Zeiss (2 mentions)
Miniflex 2, by Rigaku (2 mentions)
Axio observer d1 microscope, by Zeiss (1 mentions)
Hyclone, by Cytiva (1 mentions)
Stemi 2000 c stereoscopic microscope, by Zeiss (1 mentions)
Whatman, by Cytiva (1 mentions)
Axio observer zi, by Zeiss (1 mentions)
Dm3000, by Leica (1 mentions)
Microscope imaging software, by Leica (1 mentions)
Matlab 2007b, by MathWorks (1 mentions)
Matlab image processing toolbox, by MathWorks (1 mentions)
Efficient navigation zen , by Zeiss (1 mentions)
Imagej, by Zeiss (1 mentions)
43 protocols using axiovision le
1
Spheroid Formation Assay Protocol
2
Barley Germination Assay with ABA
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Thirty seeds of the barley cv. “Sebastian” and the hvcbp20.ab mutant were planted in a Petri dish plate (Φ = 90 mm) containing four layers of “Whatman” filters that had been soaked with 5 mL of sterile distilled water with or without 100 μM ABA. The plates were chilled at 4°C in the dark for 4 days (stratified) and moved to 22°C with a 16-h-light/8-h-dark cycle. On the second day after stratification (DAS), the Whatman paper filters were replaced with fresh ones. The seeds that were used for these experiments were harvested and stored at the same time. The percentage of seed germination was scored on the 1st, 2nd, 3rd, and 4th DAS. Germination was defined as the visible emergence of the radicle through the seed coat. The analyses were performed using a Stemi 2000-C stereoscopic microscope (Carl Zeiss) with an attached camera (Canon). In order to document the results, AxioVision LE (Carl Zeiss) software was used. The experiment was performed in three biological replicates, with 90 seeds per replicate (each replicate was performed using 3 plates, each containing 30 seeds).
3
Microscopic Fiber Diameter Analysis
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Fiber diameters were measured using a Zeiss Axio Observer ZI inverted microscope (Zeiss, Stuttgart, Germany) with a phase-contrast 20x objective lens. The quantification was completed using the Axiovision LE software (Zeiss, Stuttgart, Germany).
4
Olfactory Apparatus Histology in P. lanceolatus
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After procurement of the gonadal tissue, the dorsolateral part of the snout in the same anesthetized P. lanceolatus was again dissected out. The unilamellar olfactory apparatus was separately fixed in 4% paraformaldehyde in 0.1 (M) phosphate buffer (pH 7.3) at 4°C for 2 h. The fixed tissues were then washed in the same buffer (3 changes at 30 min of interval) and cryoprotected in 15%–30% sucrose solution in 0.1 (M) phosphate buffer for 24 h at 4°C. The frozen sections (thickness: 15–20 μm) were cut using cryostat (Leica CM 1850; Leica Biosystems Nussloch GmbH, Germany). The sections were separately incubated with Acridine Orange (AO) solution (6 μg/ml in 0.1 M phosphate buffer [pH 7.3] at 4°C for 15–30 min) and ethidium bromide (EB) solution (7 μg/ml in 0.1 M phosphate buffer [pH. 7.3] at 4°C for 30–45 min). The incubated sections washed in the same buffer (3 changes), mounted on glass slides (equal volume of glycerol and buffer is used as mounting medium), and examined under light microscope (LM: Primo Star, Carl Zeiss, GmbH, Germany) and fluorescence microscope (Leica DM 3000; Leica Microsystems) through Blue filter. The acquired images were analyzed by AxioVision LE; Version 4.3.0.101; Carl Zeiss, Germany (for LM study) and Microscope Imaging Software; Leica Application Suite Advanced Fluorescence for fluorescence microscopical data.
5
Contractility Analysis of hiPSC-CMs
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Contractility of hiPSC-CMs was recorded using a phase contrast inverted microscope (Axio Vert.A1) equipped with Zeiss AxioCam digital camera system, at 40 × magnification with recordings of 30 s for each sample at 100 ms interval between frames. Videos were processed and exported using Zeiss AxioVision LE imaging software. Video-based analysis of contractility parameters was performed with Matlab R2016b software as previously described (Huebsch et al., 2015 (link)).
6
Quantifying Neurite Lengths in Cell Cultures
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Mean neurite lengths were determined from the normal size high resolution neurite-enhanced pictures taken for morphology analyses (20x magnification) using an image analysis app (Matlab Image Processing Toolbox; MatLab 2007b, Mathworks, USA). Briefly, neurite length was defined as the Euclidean distance in pixels or a combination of Euclidean distances between the cell body and the end of a neurite. The longest path was always chosen when a neurite had several branches. The length in pixels was then calibrated based on the length bar provided by the microscope image treatment software (AxioVision LE, Carl Zeiss, DE). For each experimental condition, 15 to 30 neurites were measured for each picture (4 × 10−3 cm²) and at least 5 pictures were analyzed per condition performed. Those steps were done three independent times in duplicate. The mean neurite length for each picture analyzed within a replicate was weighted by the number of measurement taken. For each independent experiment, the mean was calculated as the weighted mean (relative to the inverse of the variance) of each replicate. Finally, the overall mean was calculated as the weighted mean (relative to the inverse of the variance) of each of the three independent experiments. Overall, a total of at least 300 neurite length measurements were obtained for each experimental condition.
7
Imaging and Quantifying CD41+ Cells in Zebrafish
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Live embryos were imaged using a Zeiss Discovery V8 microscope with Axiovision LE software; CD41:GFP+ cells were counted manually using Fiji software. For confocal microscopy, embryos were treated with 0.003% phenylthiourea in E3 and imaged on a Zeiss laser scanning LSM880 inverted confocal microscope after mounting in 3% methylcellulose using the 20 × 0.8 M27 Plan-Apochromat (Zeiss) objective. Image analysis was performed with ImageJ, Fiji, and/or Zen (Zeiss) programs.
8
Multimodal Image Analysis Techniques
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Three-dimensional region-of-interest analysis on PET images was performed with AsiPRO VM 5.0 (Concorde Microsystems) and in-house software. Model fits to this data were performed using the SAAM II software package (University of Washington, Seattle, WA) and in-house software (developed by B.J.B.). Widefield and confocal microscopy images were evaluated using ImageJ (NIH, http://rsb.info.nih.gov/ij/ ), AxioVision LE (Zeiss), and Amira 4.1 (Visage Imaging, Inc.) software. Graphs were constructed and statistical data were evaluated using Graphpad Prism 3.0 (Graphpad Software, Inc.). Statistical comparison between 2 experimental groups was performed using a t test (unpaired comparison).
9
Measuring Aphid Embryo Dimensions
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The size of aphid embryos was determined using the Microscope Software AxioVision LE based on photos taken with a confocal microscope (Zeiss LSM710, Carl Zeiss Microscopy GmbH, Jena, Germany). The total length of the embryo and the length of the third leg were measured. Where the embryo was distorted, the length was estimated as a sum of several linear measurements.
10
Fluorescent Microscopy Imaging Protocol
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Cells were observed in an inverted AxioVert CFL40 Zeiss microscope equipped with an HBO 50 mercury lamp and reflectors, under brightfield or fluorescence filter sets for YFP (excitation 488 nm, emission 517 nm). Image acquisition was performed using the Fluorescence Lite software module of AxioVision LE (Carl Zeiss, Germany).
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