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Migg2b

Manufactured by BioXCell
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MIgG2b is a type of monoclonal antibody that belongs to the IgG2b subclass. It is a laboratory reagent commonly used in various research applications.

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Lab products found in correlation

Anti ctla4 clone 9d9, by BioXCell (1 mentions) Nod shiltj mice, by Jackson ImmunoResearch (1 mentions) Diastix, by Bayer (1 mentions) Nk1.1 af700, by BioLegend (1 mentions) Cd45 pe cy7, by BioLegend (1 mentions) Cd3 fitc, by BioLegend (1 mentions) Cd8 percp, by BioLegend (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by BioLegend (1 mentions) Lsrfortessa, by Beckman Coulter (1 mentions) Cd4 bv711, by BioLegend (1 mentions)

2 protocols using migg2b

1

Preventing Diabetes in NOD Mice

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Female nonobese diabetic (NOD)/ShiLtJ mice (The Jackson Laboratory, stock #001976) were maintained with free access to standard food and water. Nine-week-old female mice confirmed to be prediabetic (negative for glucosuria) were randomized and dosed with muC5H9v2 mAb, CX-630 Pb-Tx, or mouse IgG2a (BIW) by intraperitoneal injection. In the combination study, 5-week-old prediabetic female NOD mice were injected intraperitoneally with muC5H9v2 mAb, CX-630 Pb-Tx, or mIgG2a at 10 mg/kg on days 0, 4, and 7 in combination with either anti–CTLA-4 clone 9D9 (Bio X Cell, catalog #BP0164) or mIgG2b (Bio X Cell, catalog #BP0086) at 10 mg/kg. Body weights and urinary glucose levels were monitored daily. Urinary glucose was monitored daily using a glucose test strip (Diastix; Bayer Corporation). Blood obtained by means of a tail nick was tested for glucose with an Alpha TRAK 2 test strip inserted into a glucometer (Abbott Animal Care, now owned by Zoetis). Any animal with detectable glucose in urine (≥100 mg/dL) was immediately tested for blood glucose level. Animals with blood glucose levels ≥250 mg/dL for 2 consecutive days were designated as diabetic and euthanized by CO2 inhalation.
Assi H.H., Wong C., Tipton K.A., Mei L., Wong K., Razo J., Chan C., Howng B., Sagert J., Krimm M., Diep L., Jang A., Nguyen M.T., Lapuyade N., Singson V., Villanueva R., Paidhungat M., Liu S., Rangan V., Vasiljeva O., West J.W., Richardson J.H., Irving B., Daniel D., Belvin M, & Kavanaugh W.M. (2021). Conditional PD-1/PD-L1 Probody Therapeutics Induce Comparable Antitumor Immunity but Reduced Systemic Toxicity Compared with Traditional Anti–PD-1/PD-L1 Agents. Cancer Immunology Research, 9(12), 1451-1464.
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2

Checkpoint Blockade Immunotherapy Protocols

Cited 1 time
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For checkpoint blockade: Anti-CTLA-4 (9D9), αPD-1 (RMP1-14), Rat Ig, and mIgG2b (controls) used in vivo were purchased from Bio X cell. For each 100 μl dose (suspended in PBS) 100 μg of 9D9, 250 μg of RMP114, 250 μg of Rat Ig, and 100 μg of mIgG2b was used (28 (link)).
Tumor dissociation was performed as described previously (29 (link)). For FACS, samples were acquired using an LSRFortessa instrument (Beckman Coulter) and analysis was performed using Flow Jo. The following fluorochrome-labeled anti-mouse Abs were purchased from Biolegend: CD3 FITC (#100204), CD4 BV711 (#001549), CD8 PerCP (#100731), CD45 PE-Cy7 (#552848), NK1.1 AF700 (#560515), and DAPI (#422801).
Shields B.D., Koss B., Taylor E.M., Storey A.J., West K.L., Byrum S.D., Mackintosh S.G., Edmondson R., Mahmoud F., Shalin S.C, & Tackett A.J. (2019). Loss of E-cadherin inhibits CD103 anti-tumor activity and reduces checkpoint blockade responsiveness in melanoma. Cancer research, 79(6), 1113-1123.
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