Uas flp

Manufactured by BD

Sourced in United States

The UAS-FLP is a laboratory equipment designed for fluid handling and processing. It is a versatile system that can be used for a variety of laboratory applications. The core function of the UAS-FLP is to facilitate the transfer, mixing, and processing of fluids in a controlled and automated manner.

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Lab products found in correlation

4 protocols using uas flp

Genetic Manipulation of Drosophila Stem Cells

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The following stocks were used in this study: UAS-esg-RNAi: 1#, TRiP HMS02538 and 3# TRiP JF03134 (Tsinghua Fly Center); 2# (v9793, VDRC); UAS-sc-RNAi: 1# TRiP JF02104 (THU2205, Tsinghua Fly Center) and 2# TRiP GL01130 (THU4591, Tsinghua Fly Center); UAS-esg-3HA and UAS-sc-3HA (FlyORF); Su(H)GBE-Gal4 and Dl-Gal4 (gift from Xiankun Zeng and Steven Hou, National Cancer Institute, USA); esg-LacZ (#10359, BDSC); UAS-Notch-RNAi, rab3-Gal4, tub-Gal80^ts, UAS-Flp, Act < stop < LacZ, UAS-esg and UAS-sc were all obtained from BDSC.
3R:11371635–11372788 was amplified from the DNA extracted from wild-type flies, and was then cloned into the C4PLacZ plasmid using the KpnI and NotI sites.

Li Y., Pang Z., Huang H., Wang C., Cai T, & Xi R. (2017). Transcription Factor Antagonism Controls Enteroendocrine Cell Specification from Intestinal Stem Cells. Scientific Reports, 7, 988.

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Genetic Tools for Drosophila Developmental Studies

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w¹¹¹⁸ (wild type, WT), stj-Gal4 (GMR 76H09, BDSC 46974), prc-Gal4 (Chartier et al., 2002 (link)), odd-Gal4 (Levy and Larsen, 2013 (link)), GRM13B08-Gal4 (BDSC 48546), UAS-Nls-LacZ (BDSC 6451), UAS-G-Trace (BDSC 28280), Hs-Flpm5 (BDSC 81068), UAS-Flybow1.1b (BDSC 56803), UAS-mCD8-GFP (BDSC5137), Handc-mCherry (Paululat and Heinisch, 2012 (link)), Handc-GFP (Sellin et al., 2006 (link)), 76E11-Gal4 (BDSC 39933), UAS-Antp (BDSC 7301), UAS-Ubx and UAS-AbdA (Michelson, 1994 (link)), UAS-RNAi-Ubx (BDSC 34993), Klf15 mutant (BDSC 18979), UAS-RNAi-Klf15 (BDSC 27075), UAS-FLP (BDSC 8205), Act-FRT-Stop-FRT-Gal4 UAS-GFP (BDSC 4411), eme-Gal4 (Han et al., 2002 (link)).

Morin-Poulard I., Destalminil-Letourneau M., Bataillé L., Frendo J.L., Lebreton G., Vanzo N, & Crozatier M. (2022). Identification of Bipotential Blood Cell/Nephrocyte Progenitors in Drosophila: Another Route for Generating Blood Progenitors. Frontiers in Cell and Developmental Biology, 10, 834720.

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Drosophila Genetic Stock Database

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All the genotypes analysed in this study are listed in Supplementary Table 1, in supplementary information. The following stocks were obtained from the Bloomington Drosophila Stock Center (BDSC), the Vienna Drosophila Resource Center (VDRC) or are described in Flybase: ci-GAL4 (Flybase #FBti0076751), hh-GAL4 (Flybase #FBti0017278), rn GAL4 (BDSC #7405), tub-GAL4 (BDSC #5138), pdm2-GAL4 (BDSC #49828), nub-GAL4 (Flybase #FBti0150342), FRT42D (BDSC #1802), FRT42D ubi-GFP (BDSC #5626), TRE-GFP (BDSC #59010), UAS-GFP (BDSC #35786), UAS-FLP (BDSC #4540), UAS-P35 (Flybase #FBtp0001646), UAS-HTT25Q Cerulean (BDSC #58360), UAS-HTT96Q-Cerulean (BDSC #56772) UAS-GFP-mCherry-Atg8a (BDSC #37749), UAS-Rheb (BDSC #9688), UAS-GADD34 (BDSC #76250), UAS-Atg1^RNAi (BDSC #44034), UAS-Rheb^RNAⁱ (BDSC #33966), UAS-Xrp1^RNAi (BDSC #34521), UAS Rpn2^RNAi (VDRC #106457), UAS-Rpt6^RNA (VDRC #49244), RPL5^2d2 (BDSC #25907), RPL14¹ (BDSC #2247), RPS13¹ (BDSC #2246), Dp(2:3)Cam14T (BDSC #4519).

Recasens-Alvarez C., Alexandre C., Kirkpatrick J., Nojima H., Huels D.J., Snijders A.P, & Vincent J.P. (2021). Ribosomopathy-associated mutations cause proteotoxic stress that is alleviated by TOR inhibition. Nature cell biology, 23(2), 127-135.

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Drosophila Genetic Stock Database

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