For measurement in the serum, blood was withdrawn from the mouse facial vein at indicated time points in the study, left undisturbed at 4 °C for 4 h to clot, and centrifuged at 1000 g for 10 min. The resultant supernatant was collected for subsequent analysis. For the measurement in tissues, heart tissues were homogenized in pre‐cooled PBS and centrifuged at 5000 g for 10 min and the resultant supernatant was collected for downstream analyses. In the case of BMDM, the cell supernatants were collected for downstream analyses. The GABA level was measured by using an ELISA kit (RK09121, Abclonal, China) following the manufacturer's instructions. The levels of cTnT (F7649B, Yutong, China), IL‐1β (RK00006, Abclonal, China), TNF‐α (RK00027, Abclonal, China), IL‐6 (RK00008, Abclonal, China), and IFN‐γ (RK00019, Abclonal, China) were detected by using ELISA kits following the manufacturer's instructions.
Rk00019
Manufactured by ABclonal
Sourced in China
RK00019 is a laboratory equipment product offered by ABclonal. It serves as a critical tool for conducting various scientific experiments and research activities. The core function of this product is to facilitate precise and reliable measurements, ensuring accurate data collection and analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rk00019
1
Serum and Tissue GABA and Inflammation Biomarker Assay
2
Cytokine Profiling of Mouse Tumors
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mouse tumors were homogenized by stainless steel beads in 1% RIPA buffer (0.5% sodium deoxycholate, 50 mmol/L Tris-base, pH 7.4, 150 mmol/L NaCl, 1% Triton X-100 and 0.1% SDS). ELISA array was performed using the mouse IFN-γ (RK00019, ABclonal, China), mouse IL-2 (RK00007, ABclonal, China) and mouse TNF-α (RK00027, ABclonal, China) ELISA Kit according to the manufacturer's instructions.
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