Cary 50 Scan spectrophotometer (Varian) or Specord 250 Plus (Analytik Jena) using 1 cm quartz cuvettes (Hellma Analytics); λmax (log ε) in nm. Both 1H- and 13C-NMR spectra were carried out at 298 K in D2O or CD3OD and at 500 MHz or 126 MHz, respectively. The 1H-NMR spectra were performed in an AVANCE NEO 500 MHz spectrometer (Bruker) using a 5 mm-z-gradient RT-BBI probehead; δ in ppm relative to HDO (δ 4.79; corresponds to TMS (δ 0.00)) or CHD2OD (δ 3.31; corresponds to TMS (δ 0.00)), J in Hz. Spectra in D2O were presaturated. The 13C-NMR spectra were performed in an AVANCE NEO 500 MHz spectrometer (Bruker) using a 5 mm z-gradient CP-BBO probehead; δ in ppm relative to CD3OD (δ 49.0; corresponds to TMS (δ 0.0)), J in Hz.
Avance neo 500 mhz spectrometer
Manufactured by Bruker
Sourced in Germany
The AVANCE NEO 500 MHz spectrometer is a high-performance nuclear magnetic resonance (NMR) instrument designed for advanced analytical applications. It features a 500 MHz superconducting magnet and provides precise and reliable data acquisition for a wide range of NMR spectroscopy experiments.
Automatically generated - may contain errors
Lab products found in correlation
Cary 50 scan spectrophotometer, by Agilent Technologies (1 mentions)
Specord 250 plus, by Analytik Jena (1 mentions)
1 cm quartz cuvette, by Hellma (1 mentions)
Ft ir 4100 spectrophotometer, by Jasco (1 mentions)
Silica gel 60 f254 aluminum plates, by Merck Group (1 mentions)
Avance 3 500 mhz spectrometer, by Bruker (1 mentions)
6 protocols using avance neo 500 mhz spectrometer
1
Comprehensive Spectroscopic Characterization
2
NMR Characterization of Dissolved Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The samples were separately dissolved in 0.5 mL of DMSO, and then 1H NMR was recorded on an AVANCE NEO 500 MHz spectrometer (Bruker, Mannheim, Germany) with a 5 mm CPTCI 1H probe [22 (link)].
3
Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals and reagents (AR) were procured from commercial sources. Melting points were determined by calibrated digital melting point apparatus (Make-Labline). TLC analysis was carried out on precoated Silica gel 60 F254 aluminum plates procured from Merck, Germany, and spots were visualized by UV light and/or by iodine vapors. Fourier-transform infrared (FT-IR) spectra for all the synthesized intermediates and final compounds were recorded on a JASCO 4100 FTIR spectrophotometer in the range of 4000–500cm−1. Proton nuclear magnetic resonance (1H NMR) spectra and carbon nuclear magnetic resonance (13C NMR) spectra were scanned using Bruker Avance Neo 500MHz spectrometer using DMSO-d6/CDCl3 as solvent. Chemical shift (δ) values are reported in ppm with TMS as an internal standard. Mass spectra were recorded on a Synapt-XS using the TOF MS ES+ method.
4
Polymer Composition Analysis by NMR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The chemical compositions of the polymer were determined by 1H NMR spectra (Bruker Avance NEO 500 MHz spectrometer, Bruker, Germany). The 500 MHz 1H NMR spectra were recorded in CDCl3 solution (Sigma-Aldrich, USA) of 20 mg/mL PHA at 25 °C.
5
Lyophilized Alginate Solutions Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Solutions containing commercial and extracted alginates (20.2 mg/mL) were prepared in deuterated water (D2O 99.9%) and then lyophilized. Subsequently, 100 μL of a solution of TSP-d4 (sodium 2,2,3,3-tetradeutero-3-trimethylsilylpropionate) and 900 μL of D2O (99.9%) were added to each lyophilizate, dissolved, and 600 μL was taken for analysis. 1H-NMR study was carried out on a Bruker Avance NEO 500 MHz spectrometer (Bruker, Billerica, MA, USA) at 80 °C. A 90° pulse and 32 sweeps were used, with a waiting time between sweeps of 20 s. Overall, 64k points were used to register the free induction decay (FID). 1H-NMR spectra were recorded both with and without presaturation of the residual solvent signal (semi-deuterated water, DHO).
6
NMR Spectroscopy of Fluticasone Propionate
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fluticasone propionate
spectra were recorded at 298 K on a Bruker Avance NEO 500 MHz spectrometer
(Bruker Biospin) with a 5 mm BBFO probe with standard transmitter
routing and equipped with a z-gradient coil with a maximum nominal
gradient strength of 50 G cm–1. Fluorohydrin spectra
were recorded at 298 K on a Bruker Avance III 500 MHz spectrometer
(Bruker Biospin) with a 5 mm BBO probe with standard transmitter routing
equipped with a z-gradient coil with a maximum nominal gradient strength
of 53.5 G cm–1. The durations of the hard 1H and 19F 90° pulses were calibrated before every
sample analysis. iBURP242 (link)19F-selective and rSNOB43 (link)1H-selective
180° shaped pulses were used. The delay Δ was set to 9.1–11.8
ms, depending on the selected 19F–1H
pair and their corresponding JHF coupling.
All gradient pulses (G1–G4) had a smoothed
square shape (SMSQ) with 100 digitized points. Gradients G1–G4 had strengths of 23%, 3%, 79%, and 4%, respectively,
as a fraction of the nominal maximum amplitudes given above. Following
acquisition, IPAP data were processed using the Bruker “split”
AU macro. All raw data, experimental parameters, Mathematica notebooks
used for the accurate measurements of JHF couplings, and Bruker pulse program codes used in this work are
available at DOI: 10.48420/21975908. Further details are provided
in theSI .
spectra were recorded at 298 K on a Bruker Avance NEO 500 MHz spectrometer
(Bruker Biospin) with a 5 mm BBFO probe with standard transmitter
routing and equipped with a z-gradient coil with a maximum nominal
gradient strength of 50 G cm–1. Fluorohydrin spectra
were recorded at 298 K on a Bruker Avance III 500 MHz spectrometer
(Bruker Biospin) with a 5 mm BBO probe with standard transmitter routing
equipped with a z-gradient coil with a maximum nominal gradient strength
of 53.5 G cm–1. The durations of the hard 1H and 19F 90° pulses were calibrated before every
sample analysis. iBURP242 (link)19F-selective and rSNOB43 (link)1H-selective
180° shaped pulses were used. The delay Δ was set to 9.1–11.8
ms, depending on the selected 19F–1H
pair and their corresponding JHF coupling.
All gradient pulses (G1–G4) had a smoothed
square shape (SMSQ) with 100 digitized points. Gradients G1–G4 had strengths of 23%, 3%, 79%, and 4%, respectively,
as a fraction of the nominal maximum amplitudes given above. Following
acquisition, IPAP data were processed using the Bruker “split”
AU macro. All raw data, experimental parameters, Mathematica notebooks
used for the accurate measurements of JHF couplings, and Bruker pulse program codes used in this work are
available at DOI: 10.48420/21975908. Further details are provided
in the
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!