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Recombinant murine il 13

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Recombinant murine IL-13 is a protein produced using recombinant DNA technology. It is a member of the interleukin-13 family of cytokines.

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Lab products found in correlation

Cc 3118, by Lonza (2 mentions) Pneumacult ali medium, by STEMCELL (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Recombinant murine il 5, by Thermo Fisher Scientific (1 mentions) Anti mouse f4 80, by Thermo Fisher Scientific (1 mentions) Anti α sma, by Thermo Fisher Scientific (1 mentions) Antibody against actin, by Santa Cruz Biotechnology (1 mentions) Anti cd31, by Thermo Fisher Scientific (1 mentions) Aicar, by Merck Group (1 mentions) Pe conjugated anti mouse cd86, by BioLegend (1 mentions) Fitc conjugated anti mouse f4 80, by BioLegend (1 mentions) Pe conjugated anti mouse cd206, by BioLegend (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Metformin, by Merck Group (1 mentions) Matrigel, by BD (1 mentions) Fitc annexin 5 apoptosis detection kit 1, by BD (1 mentions) Jetprime transfection agent, by Polyplus Transfection (1 mentions) Recombinant murine ifn γ, by Thermo Fisher Scientific (1 mentions) Recombinant murine il 4, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

IL-13 (429 citations) Murine IL-13 (4 citations) Recombinant IL-13 (3 citations) Recombinant murine IL-3 (213-13, (2 citations)

6 protocols using recombinant murine il 13

1

Quantifying IL-5 and IL-13 Secretion

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The secretion of IL-5 and IL-13 was assessed by enzyme-linked immunosorbent assays (ELISA) according to the manufacturer protocol using as standards recombinant murine IL-5 (PeproTech #215-15) and recombinant murine IL-13 (PeproTech #210-13).
de Lucía Finkel P., Sherwood C., Saranchova I., Xia W., Munro L., Pfeifer C.G., Piret J.M, & Jefferies W.A. (2021). Serum free culture for the expansion and study of type 2 innate lymphoid cells. Scientific Reports, 11, 12233.
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2

Airway Basal Stem Cell Differentiation

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Cells were cultured and expanded in complete SAGM (small airway epithelial cell growth medium; Lonza, CC-3118) containing TGF-β/BMP4/WNT antagonist cocktails and 5 μM Rock inhibitor Y-27632 (Selleckbio, S1049). To initiate air–liquid interface (ALI) cultures, airway basal stem cells were dissociated from mouse tracheas and seeded onto transwell membranes. After reaching confluence, media was removed from the upper chamber. Mucocilliary differentiation was performed with PneumaCult-ALI Medium (StemCell, 05001). Differentiation of airway basal stem cells on an air–liquid interface was followed by directly visualizing beating cilia in real time after 10–14 days.
Once air-liquid cultures were fully differentiated, as indicated by beating cilia, treatment cultures were supplemented with 25ng/mL of recombinant murine IL-13 (Peprotech-stock diluted in water and used fresh) diluted in PneumaCult-ALI Medium, while control cultures received an equal volume of water for 72 hours. After treatment, whole ALI wells were fixed in 4% PFA, immunostained in whole mount using the same buffers and imaged with a confocal microscope as described above.
Montoro D.T., Haber A.L., Biton M., Vinarsky V., Lin B., Birket S., Yuan F., Chen S., Leung H.M., Villoria J., Rogel N., Burgin G., Tsankov A., Waghray A., Slyper M., Waldmann J., Nguyen L., Dionne D., Rozenblatt-Rosen O., Tata P.R., Mou H., Shivaraju M., Bihler H., Mense M., Tearney G.J., Rowe S.M., Engelhardt J.F., Regev A, & Rajagopal J. (2018). A revised airway epithelial hierarchy includes CFTR-expressing ionocytes. Nature, 560(7718), 319-324.
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3

Macrophage Polarization Dynamics

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Metformin, LPS, AICAR, and phorbol 12-myristate 13-acetate (PMA) were obtained from Sigma (St. Louis, MO). Recombinant murine IL-13 was purchased from PeproTech (Rocky Hill, NJ). Mouse recombinant M-CSF and antibodies against AMPKα1 and p-AMPKα1 were from Cell Signaling Technology (Beverly, MA, USA). Antibody against Actin was purchased from Santa Cruz Biotechnology (CA, USA). Antibodies for flow cytometry including PE-conjugated anti-mouse CD206, PE-conjugated anti-mouse CD86 and FITC-conjugated anti-mouse F4/80 were purchased from Biolegend (San Diego, CA, USA). For immunofluorescence, first antibodies including anti-mouse F4/80, anti-CD31, anti-αSMA, and FITC-conjugated anti-mouse CD206 were from eBioscience, Abcam, Sigma and Biolegend respectively, while secondary antibodies including anti-Rat and anti-mouse were from life technology. FITC Annexin V Apoptosis Detection Kit I and matrigel were purchased from BD (San Jose, CA, USA). JetPrime transfection agent was obtained from Polyplus. Clodronate liposomes and PBS liposomes were purchased from ClodronateLiposomes.com (Amsterdam, Netherlands).
Ding L., Liang G., Yao Z., Zhang J., Liu R., Chen H., Zhou Y., Wu H., Yang B, & He Q. (2015). Metformin prevents cancer metastasis by inhibiting M2-like polarization of tumor associated macrophages. Oncotarget, 6(34), 36441-36455.
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4

Murine IL-13 in Cryoinjury Model

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Recombinant murine IL‐13 (Peprotech, 210‐13) was administered intraperitoneally, 3 (200 ng), 4 (200 ng), 5 (400 ng), and 6 (400 ng) days after cryoinjury and mice were sacrificed 7 days after injury.
Malek Mohammadi M., Kattih B., Grund A., Froese N., Korf‐Klingebiel M., Gigina A., Schrameck U., Rudat C., Liang Q., Kispert A., Wollert K.C., Bauersachs J, & Heineke J. (2017). The transcription factor GATA4 promotes myocardial regeneration in neonatal mice. EMBO Molecular Medicine, 9(2), 265-279.
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5

Airway Basal Stem Cell Differentiation

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Cells were cultured and expanded in complete SAGM (small airway epithelial cell growth medium; Lonza, CC-3118) containing TGF-β/BMP4/WNT antagonist cocktails and 5 μM Rock inhibitor Y-27632 (Selleckbio, S1049). To initiate air–liquid interface (ALI) cultures, airway basal stem cells were dissociated from mouse tracheas and seeded onto transwell membranes. After reaching confluence, media was removed from the upper chamber. Mucocilliary differentiation was performed with PneumaCult-ALI Medium (StemCell, 05001). Differentiation of airway basal stem cells on an air–liquid interface was followed by directly visualizing beating cilia in real time after 10–14 days.
Once air-liquid cultures were fully differentiated, as indicated by beating cilia, treatment cultures were supplemented with 25ng/mL of recombinant murine IL-13 (Peprotech-stock diluted in water and used fresh) diluted in PneumaCult-ALI Medium, while control cultures received an equal volume of water for 72 hours. After treatment, whole ALI wells were fixed in 4% PFA, immunostained in whole mount using the same buffers and imaged with a confocal microscope as described above.
Montoro D.T., Haber A.L., Biton M., Vinarsky V., Lin B., Birket S., Yuan F., Chen S., Leung H.M., Villoria J., Rogel N., Burgin G., Tsankov A., Waghray A., Slyper M., Waldmann J., Nguyen L., Dionne D., Rozenblatt-Rosen O., Tata P.R., Mou H., Shivaraju M., Bihler H., Mense M., Tearney G.J., Rowe S.M., Engelhardt J.F., Regev A, & Rajagopal J. (2018). A revised airway epithelial hierarchy includes CFTR-expressing ionocytes. Nature, 560(7718), 319-324.
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6

Murine Macrophage Polarization Protocol

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Mouse peritoneal macrophages from no Cre mice (Lyz2 Cre-/-RhoA flox/flox ), further called the wild type or RhoA deleted Lyz2 Cre+/-RhoA flox/flox mice, forther called the KO were purified by overnight incubation in Dulbecco's Modified Eagle Medium (DMEM) with 10% fetal bovine serum (FBS), 100U/ml penicillin and100 mg/ml streptomycin (all from Thermo Fisher Scientific, Waltham, MA, USA) and subsequent removal of free-floating cells. For M1 polarization, macrophages were incubated for 24 hr either with 20ng/ml of recombinant murine IFN-γ (Peprotech, Rocky Hill, NJ, USA) and100ng/ml of Lipopolysaccharide (LPS) (Sigma Aldrich,St. Louis, MO, USA) or for M2 polarization, with 20ng/ml of recombinant murine IL-4 (Peprotech) and 10ng/ml of recombinant murine IL-13 (Peprotech) as described previously (Liu et al, 2016a.b) . Macrophages were grown either in 6/12 well plastic petri dishes or in glass chamber slides.
Chen W., Zhao Y., Li X.C., Kubiak J.Z., Ghobrial R.M, & Kloc M. (2017). Rho-specific Guanine nucleotide exchange factors (Rho-GEFs) inhibition affects macrophage phenotype and disrupts Golgi complex. The international journal of biochemistry & cell biology, 93.
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