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Anti cd197 ccr7 pe

Manufactured by BioLegend
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Anti-CD197 (CCR7) PE is a monoclonal antibody product from BioLegend. It recognizes the CD197 (CCR7) cell surface receptor, which is involved in lymphocyte trafficking and homing.

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Lab products found in correlation

Anti cd8a percp cy5.5 hit8a, by BioLegend (1 mentions) Anti cd8 fitc, by BD (1 mentions) Facs aria 1 sorter, by BD (1 mentions) Anti cd95 apc, by BioLegend (1 mentions) Anti cd45ro pe cy7, by BD (1 mentions) Anti cd45ra bv421, by BD (1 mentions) 7 aminoactinomycin d 7 aad, by BD (1 mentions)

2 protocols using anti cd197 ccr7 pe

1

Multiparameter Immunophenotyping of T-cells

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Fluorescein-conjugated anti-human mAbs, including anti-CD3 FITC (clone: UCHT1), anti-CD4 V500 (RPA-T4), anti-CD8 APC/Cy7 (SK1), anti-CD56 PE (B159), and anti-CD279 (PD-1) PE (EH12.1, also known as EH12) were purchased from BD Pharmingen. Meanwhile, the other anti-human mAbs including anti-CD3 APC (HIT3a), anti-CD8a PerCP/Cy5.5 (HIT8a), anti-CD38 FITC (HIT2), anti-CD63 FITC (H5C6), anti-CD69 APC (FN50), anti-CD122 (IL-2Rβ) APC (TU27), anti-CD183 (CXCR3) PE/Cy7 (G025H7), anti-CD183 (CXCR3) APC (G025H7), anti-CD192 (CCR2) APC/Cy7 (K036C2), anti-CD196 (CCR6) PE (G034E3), anti-CD197 (CCR7) PE (G043H7), anti-CD276 (B7-H3) PE (DCN.70), anti-CD45RO FITC (UCHL1), anti-CD197 (CCR7) PE/Cy7 (G043H7), anti-IFN-γ APC/Cy7 (4S.B3), anti-IL-4 PE/Cy7 (MP4-25D2), anti-IL-17A FITC (BL168), and anti-FOXP3 PE (206D) were purchased from BioLegend.
Zeng W., Liu Z., Liu X., Zhang S., Khanniche A., Zheng Y., Ma X., Yu T., Tian F., Liu X.R., Fan J, & Lin Y. (2017). Distinct Transcriptional and Alternative Splicing Signatures of Decidual CD4+ T Cells in Early Human Pregnancy. Frontiers in Immunology, 8, 682.
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2

Isolation of Naïve CD8+ T Cells

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Naïve CD8+ T cells were sorted using a FACSAria I Sorter (BD Biosciences) by staining the isolated PBMCs with anti-CD8 FITC (BD Biosciences), anti-CD197 (CCR7) PE, anti-CD95 APC (both from BioLegend), anti-CD45RO PE-Cy7, anti-CD45RA BV421 and 7-Amino-Actinomycin D (7-AAD) (all from BD Biosciences). PBMCs were sorted into naïve T cells (TN, CD8+ CD197+ CD95- CD45RO- CD45RA+), collected into RPMI 1640 medium containing 1% FBS and counted manually before starting the differentiation experiments.
Kirchmair A., Nemati N., Lamberti G., Trefny M., Krogsdam A., Siller A., Hörtnagl P., Schumacher P., Sopper S., Sandbichler A., Zippelius A., Ghesquière B, & Trajanoski Z. (2023). 13C tracer analysis reveals the landscape of metabolic checkpoints in human CD8+ T cell differentiation and exhaustion. Frontiers in Immunology, 14, 1267816.
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