Cell culture dishes

Human ovarian cancer cell lines PA-1 (p53 wild type) and SKOV-3 (p53 null type) were purchased from the American Type Culture Collection (Rockville, MD, USA). PA-1 cells were maintained in MEM and SKOV-3 cells were maintained in RPMI 1640 media. All of the media was supplemented with 10% fetal bovine serum (FBS) (Gibco BRL, Life Technologies, Grand Island, NY, USA), 100 mg/ml penicillin/streptomycin (P/S) (Gibco), and maintained in cell culture dishes (SPL, Seoul, South Korea) at 37°C in a humidified atmosphere with 5% CO₂.

The human PCa cell lines, PC-3 and DU145, were purchased from the Korean Cell Line Bank (Seoul, Korea). Both cell lines were cultured using a medium (DMEM, HyClone Laboratories, Chicago, IL, USA) supplemented with 10% fetal bovine serum (FBS; RMBIO, Missoula, MT, USA), 1% penicillin G/streptomycin (Bio west, San Marcos, TX, USA), 1% HEPES (Gibco by Life Technologies, Gaithersburg, MD, USA) and 0.05% cell maxin (GenDEPOT, Katy, TX, USA) in cell culture dishes (SPL Life Science, Pocheon, Korea) at 37 °C in a humidified atmosphere containing 95% air and 5% CO₂. Both cell lines were detached by using 0.05% Trypsin-EDTA (Gibco by Life Technologies, Gaithersburg, MD, USA).