Free fatty acid detection kit

As described previously [14 (link), 15 (link)], serum alanine aminotransferase (ALT) and fasting blood glucose (FBG) levels were determined using commercially available kits (Wako, Osaka, Japan). Serum immunoreactive insulin (IRI) levels were measured using a mouse insulin ELISA kit (Funakoshi, Tokyo, Japan). Stored liver samples (100 mg) were lysed and homogenized in a 2 mL solution containing 150 mM NaCl, 0.1% Triton X-100, and 10 nM Tris using a polytron homogenizer (NS-310E; MicroTech Nichion, Tokyo, Japan) for 1 min. Hepatic triglyceride (TG), free fatty acid (FFA) and β-hydroxybutyrate contents were measured using a triglyceride detection kit (Wako), a free fatty acid detection kit (Wako), and β-hydroxybutyrate assai kit (Cayman chemical, Ann Arbor, MI, USA) respectively. Serum TG, FFA, and cholesterol levels were also measured using a triglyceride detection kit (Wako), free fatty acid detection kit (Wako), and cholesterol detection kit (Wako), respectively.

As described previously [32 (link)], serum ALT and FBG levels were determined using commercially available kits (Wako). Serum IRI levels were measured using a mouse insulin ELISA kit (Funakoshi, Tokyo, Japan). The stored liver samples (100 mg) were lysed and homogenized using a polytron homogenizer (NS-310E; MicroTech Nichion, Tokyo, Japan) for 1 min in a 2-mL solution containing 150 mM NaCl, 0.1% Triton X-100, and 10 nM Tris. Hepatic TG, FFA, and β-hydroxybutyrate levels were measured using a triglyceride detection kit (Wako), free fatty acid detection kit (Wako), and β-hydroxybutyrate assay kit (Cayman Chemical, Ann Arbor, MI, USA), respectively. Serum TG and FFA levels were also measured using a triglyceride detection kit (Wako) and a free fatty acid detection kit (Wako), respectively.

Serum alanine aminotransferase (ALT) and fasting blood glucose (FBG) levels were determined using commercially available kits (Wako, Osaka, Japan), and serum immunoreactive insulin (IRI) levels were measured using a mouse insulin ELISA kit (Funakoshi, Tokyo, Japan). Stored liver samples (100 mg) were lysed and homogenized in 2 mL of a solution containing 150 mM NaCl, 0.1% Triton X-100, and 10 nM Tris using a polytron homogenizer (NS-310E; MicroTech Nichion, Tokyo, Japan) for 1 min. Hepatic TG and FFA contents were measured using a triglyceride detection kit (Wako) and free fatty acid detection kit (Wako), respectively.