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Rt mmlv

Manufactured by Promega
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Sourced in France, United States

The RT-MMLV is a reverse transcriptase enzyme derived from Moloney Murine Leukemia Virus. It is used to synthesize complementary DNA (cDNA) from RNA templates.

Automatically generated - may contain errors

Lab products found in correlation

Trizol reagent, by Thermo Fisher Scientific (1 mentions) Trizol reagent protocol, by Thermo Fisher Scientific (1 mentions) Turbo dna free kit, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

MMLV-RT reverse transcriptase (16 citations) MMLV-RT RNaseH- (3 citations)

2 protocols using rt mmlv

1

RNA Extraction and RT-qPCR from Caecal Tissue

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Total RNA from caecal tissue was extracted by using the TRIzol® reagent (Invitrogen, by Life Technologies, Villebon sur Yvette, France). Total RNA (1 µg) was reverse transcribed with 0.5 mM of dNTP, 2 M of RT-Buffer, 0.5 μg/µL of OligodT, 0.125 U of ribonuclease inhibitor and 0.05U of RT-MMLV (Promega, France) for 1 h at 37 °C (Thermocycler PE9700, Perkin Elmer, France). cDNA was stored at −80 °C until qPCR. Biological samples from 10 hens per group were analyzed
Grandhaye J., Douard V., Rodriguez-Mateos A., Xu Y., Cheok A., Riva A., Guabiraba R., Zemb O., Philippe C., Monnoye M., Staub C., Venturi E., Barbe A., Ramé C., Dupont J, & Froment P. (2020). Microbiota Changes Due to Grape Seed Extract Diet Improved Intestinal Homeostasis and Decreased Fatness in Parental Broiler Hens. Microorganisms, 8(8), 1141.
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2

Isolating Total RNA from Plant Materials

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Total RNA was isolated using TRIzol reagent protocol (Invitrogen, Waltham, MA, USA). The RNA was treated with TURBO DNase for 30 min at 37 °C (TURBO DNA-free™ Kit, Invitrogen) followed by a DNase inactivation step. First-strand cDNA synthesis was performed on 500 ng total RNA per reaction using RT M-MLV (Promega, Madison, WI, USA) and random hexamers following the manufacturer’s protocol. Total RNA was isolated from plant materials using the same protocol.
Xu M., Mazur M., Gulickx N., Hong H., Overmars H., Tao X, & Kormelink R. (2022). Bunyaviral N Proteins Localize at RNA Processing Bodies and Stress Granules: The Enigma of Cytoplasmic Sources of Capped RNA for Cap Snatching. Viruses, 14(8), 1679.
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