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Cos 1 and hek293t cells

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COS-1 and HEK293T cells are widely used cell lines in various areas of biological research. COS-1 cells are derived from the African green monkey kidney and are commonly used for expression of recombinant proteins. HEK293T cells are derived from human embryonic kidney cells and are frequently used for virus production and transfection experiments. Both cell lines provide researchers with reliable and well-characterized tools for their investigations.

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Lab products found in correlation

Glutamax media, by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)

2 protocols using cos 1 and hek293t cells

1

Transient Transfection of COS-1 and HEK293T Cells

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COS-1 and HEK293T cells (ATCC; Manassas, VA) were grown in 24-well plates or 100mm dishes containing Glutamax media (GIBCO; Gaithersburg, MD)/10% fetal bovine serum (FBS) at 37°C/5% CO2. Plasmid DNA was transiently transfected using Optimem and Lipofectamine 2000 as per manufacturer’s instructions (Life Technologies). The morning following transfection, Optimem was replaced with Glutamax media/5% FBS and cells continued to grow for 5-24 hours. Cells were fixed with 4% paraformaldehyde for imaging studies or harvested in lysis buffer for Westerns. Experiments shown in Figures 3D-O were repeated ≥3 times with similar results.
Pathak G., Agostino M.J., Bishara K., Capell W.R., Fisher J.L., Hegde S., Ibrahim B.A., Pilarzyk K., Sabin C., Tuczkewycz T., Wilson S, & Kelly M.P. (2016). PDE11A negatively regulates lithium responsivity. Molecular psychiatry, 22(12), 1714-1724.
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2

Transient Transfection of COS-1 and HEK293T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
COS-1 and HEK293T cells (ATCC; Manassas, VA) were grown in 24-well plates or 100mm dishes containing Glutamax media (GIBCO; Gaithersburg, MD)/10% fetal bovine serum (FBS) at 37°C/5% CO2. Plasmid DNA was transiently transfected using Optimem and Lipofectamine 2000 as per manufacturer’s instructions (Life Technologies). The morning following transfection, Optimem was replaced with Glutamax media/5% FBS and cells continued to grow for 5-24 hours. Cells were fixed with 4% paraformaldehyde for imaging studies or harvested in lysis buffer for Westerns. Experiments shown in Figures 3D-O were repeated ≥3 times with similar results.
Pathak G., Agostino M.J., Bishara K., Capell W.R., Fisher J.L., Hegde S., Ibrahim B.A., Pilarzyk K., Sabin C., Tuczkewycz T., Wilson S, & Kelly M.P. (2016). PDE11A negatively regulates lithium responsivity. Molecular psychiatry, 22(12), 1714-1724.
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