Frozen sections of middle caecum were processed as previously described [33 (link), 38 (link)]. Sections were stained with one of the following mouse-anti-chicken unlabeled monoclonal antibodies: anti-CD4, anti-CD8β and anti-Bu1 (0.05 µg/ml) (Southern Biotech, provided by Biozol, Eching, Germany). The secondary anti-mouse IgG biotinylated antibodies and ABC reagent (Vectastain® Elite® ABC Kit, Vector Laboratories Inc., provided by Linaris, Wertheim-Bettingen, Germany) were applied according to the manufacturer’s instructions. The enzyme-linked ABC complex was visualized by the reaction with 3.3′-diaminobenzidine (DAB) chromagen substrate and hydrogen peroxide (DAB peroxidase substrate Kit, Vector Laboratories Inc.). Sections were examined with light microscopy. The different lymphocyte populations were evaluated by counting the number of positive stained cells per 3 crypts in the lamina propria of 5 representative microscopic fields at 200× optical magnification per animal [33 (link)].
Anti cd4
Manufactured by Southern Biotech
Sourced in Germany
Anti-CD4 is a laboratory reagent used for the detection and quantification of CD4-positive cells. It functions as a specific binding agent for the CD4 surface antigen, a marker expressed on certain T lymphocytes. This product can be utilized in various cell analysis and sorting applications.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using anti cd4
1
Caecum Lymphocyte Immunohistochemistry Protocol
2
Immunohistochemical Analysis of Lymphocyte Subsets
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Frozen sections of middle cecum, cecal tonsil, and bursa of Fabricius were processed as previously described (37 (link), 38 (link)). Sections were stained with one of the following mouse anti-chicken unlabeled monoclonal antibodies: anti-CD4, anti-CD8β, and anti-Bu1 (0.05 μg/ml; Southern Biotech, provided by Biozol, Eching, Germany). The secondary anti-mouse IgG biotinylated antibodies and ABC reagent (Vectastain Elite ABC kit; Vector Laboratories Inc., provided by Linaris, Wertheim-Bettingen, Germany) were applied according to the manufacturer's instructions. The enzyme-linked ABC complex was visualized by the reaction with 3.3′-diaminobenzidine (DAB) chromogen substrate and hydrogen peroxide (DAB peroxidase substrate kit; Vector Laboratories Inc.). Sections were examined by light microscopy. The different lymphocyte populations were evaluated by counting the number of stained cells per 3 crypts in the cecal lamina propria and in 5 representative microscopic fields at an optical magnification of ×200 in the bursa of Fabricius of each animal (38 (link)).
3
Cardiac Immune Cell Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The middle one-third of ventricles was embedded in O.C.T. compound (Sakura Finetek, Tokyo, Japan) tissue medium, frozen in liquid nitrogen and stored at À80 8C. Tissue blocks were sectioned at 6 mm. Sections were then incubated with anti-CD4 (South-ernBiotech, Birmingham, AL, USA), anti-CD8 (PharMingen, San Diego, CA, USA) or anti-renin (no. 593) antibody (each at 1-10 mg/ mL) at 4 8C. Histofine simple stain was used as a secondary antibody. Incubations with secondary antibodies were carried out at room temperature for 30 min. After incubation with avidinbiotin-horseradish peroxidase complexes, counterstaining was performed with hematoxylin. Immunostained type-and classmatched nonimmune phosphate-buffered saline was used as the negative control for each antibody [27, 28] . Measurement of immunoreactivity of myocardial infiltrating cells for CD4 and CD8 was performed in 25 randomly selected fields in heart sections at 400-fold magnification of light microscopy.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!