Mono s 5 50 gl column
The Mono S 5/50 GL column is a cation exchange chromatography column designed for protein purification. It features a bed volume of 5 ml and a column length of 50 mm. The column is composed of a rigid polymethacrylate matrix with sulfopropyl functional groups for the capture and separation of positively charged proteins.
Lab products found in correlation
16 protocols using mono s 5 50 gl column
Purification of the KIX Domain
Determination of FimCH Impurities by CEX-HPLC
Example 5
Impurities of FimCH Drug Substance by CEX-H PLC
CEX-HPLC is used for determination of the FimCH complex, unbound FimC and impurities in the final FimCH drug substance. The protein is eluted from a GE Healthcare Mono S 5/50 GL column using a gradient of 0.3 M NaCl in 20 mM MES buffer, pH 6.2 (Buffer B) (Buffer A is 20 mM MES buffer, pH 6.2). At T=0 the mobile phase is 100% Buffer A, and at T=22 minutes, the mobile phase is 100% Buffer B. The relative content of the unbound FimC and impurities is determined based on the peak areas. A representative chromatogram is provided in
Cation-exchange Chromatography for C3 Variants
Quantifying FimCH Drug Impurities by CEX-HPLC
Example 5
Impurities of FimCH Drug Substance by CEX-HPLC
CEX-HPLC is used for determination of the FimCH complex, unbound FimC and impurities in the final FimCH drug substance. The protein is eluted from a GE Healthcare Mono S 5/50 GL column using a gradient of 0.3 M NaCl in 20 mM MES buffer, pH 6.2 (Buffer B) (Buffer A is 20 mM MES buffer, pH 6.2). At T=0 the mobile phase is 100% Buffer A, and at T=22 minutes, the mobile phase is 100% Buffer B. The relative content of the unbound FimC and impurities is determined based on the peak areas. A representative chromatogram is provided in
Quantification of FimCH Impurities by CEX-HPLC
Example 5
Impurities of FimCH Drug Substance by CEX-HPLC
CEX-HPLC is used for determination of the FimCH complex, unbound FimC and impurities in the final FimCH drug substance. The protein is eluted from a GE Healthcare Mono S 5/50 GL column using a gradient of 0.3 M NaCl in 20 mM MES buffer, pH 6.2 (Buffer B) (Buffer A is 20 mM MES buffer, pH 6.2). At T=0 the mobile phase is 100% Buffer A, and at T=22 minutes, the mobile phase is 100% Buffer B. The relative content of the unbound FimC and impurities is determined based on the peak areas. A representative chromatogram is provided in
Synthesis of K48-linked Polyubiquitin Chains
Affinity Purification and Biochemical Analysis
Determination of FimCH Impurities by CEX-HPLC
Example 5
Impurities of FimCH Drug Substance by CEX-H PLC
CEX-HPLC is used for determination of the FimCH complex, unbound FimC and impurities in the final FimCH drug substance. The protein is eluted from a GE Healthcare Mono S 5/50 GL column using a gradient of 0.3 M NaCl in 20 mM MES buffer, pH 6.2 (Buffer B) (Buffer A is 20 mM MES buffer, pH 6.2). At T=0 the mobile phase is 100% Buffer A, and at T=22 minutes, the mobile phase is 100% Buffer B. The relative content of the unbound FimC and impurities is determined based on the peak areas. A representative chromatogram is provided in
Purification and Characterization of Vaa Venom
Phosphorylation of Rab8a by MST3 Kinase
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