Blood was collected in Tempus reagent (Life Technologies) at 1:2 ratio. Total RNA was extracted using the PerfectPure RNA Blood Kit (5 PRIME). Globin RNA was depleted from total RNA (1.5–2 µg) using the Mouse GLOBINclear kit (Thermo Fisher Scientific). Tissues were collected in TRI-Reagent (Sigma-Aldrich). Total RNA was extracted using the RiboPure™ Kit (Ambion). FACS sorted blood/lung cells were collected into TRI-Reagent LS (Sigma-Aldrich). Total RNA was extracted using the Purelink RNA microkit (Thermo Fisher). BAL cell pellets were obtained from pooled lavage fluid from each mouse, washed once in PBS and lysed in 350 μl RLT buffer. Lysates were passed through QIAshredder columns (QIAGEN). RNA was extracted using the RNeasy mini kit as per manufacturer’s instructions, including on-column DNase I digestion (both QIAGEN). All RNA was stored at −80 °C until use.
Mouseglobinclear kit
Manufactured by Thermo Fisher Scientific
The MouseGLOBINclear kit is a laboratory product designed for the separation and purification of mouse globin proteins from blood samples. It provides a reliable and efficient method for isolating these proteins, which are an important component in various biological research applications.
Automatically generated - may contain errors
Lab products found in correlation
Tri reagent, by Merck Group (3 mentions)
Ribopure kit, by Thermo Fisher Scientific (3 mentions)
Tempus reagent, by Thermo Fisher Scientific (3 mentions)
Rneasy mini kit, by Qiagen (1 mentions)
Purelink rna micro kit, by Thermo Fisher Scientific (1 mentions)
Tri reagent ls, by Merck Group (1 mentions)
Qiashredder column, by Qiagen (1 mentions)
Dnase 1 digestion, by Qiagen (1 mentions)
3 protocols using mouseglobinclear kit
1
RNA Extraction Techniques for Various Samples
2
RNA Extraction and Globin Depletion
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Blood was collected in Tempus reagent (Life Technologies) at 1:2 ratio.
Total RNA was extracted using the PerfectPure RNA Blood Kit (5 PRIME). Globin
RNA was depleted from total RNA (1.5–2 μg) using the Mouse
GLOBINclear kit (Thermo Fisher Scientific). Lungs were collected in TRI-Reagent
(Sigma-Aldrich). Total RNA was extracted using the RiboPure™ Kit
(Ambion). All RNA was stored at −80 °C until use.
Total RNA was extracted using the PerfectPure RNA Blood Kit (5 PRIME). Globin
RNA was depleted from total RNA (1.5–2 μg) using the Mouse
GLOBINclear kit (Thermo Fisher Scientific). Lungs were collected in TRI-Reagent
(Sigma-Aldrich). Total RNA was extracted using the RiboPure™ Kit
(Ambion). All RNA was stored at −80 °C until use.
3
RNA Extraction and Globin Depletion
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