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2 protocols using anti t7 epitope tag

1

Antibody-based Protein Interaction Assay

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The following antibodies or beads were employed in this study: anti-V5-tag magnetic beads (MBL), NiNTA beads (Qiagen), anti-V5 (Invitrogen), anti-ADAR1 (Santa Cruz Biotechnology), anti-SFPQ/PSF (abcam), anti-NONO/p54nrb (Bethyl), anti-hnRNP L (abcam), anti-NCL (Santa Cruz Biotechnology), anti-PABP (Santa Cruz Biotechnology), anti-HSP70 (Santa Cruz Biotechnology), anti-GAPDH (Millipore), anti-FLAG M2 (Sigma-Aldrich), anti-T7 epitope tag (Millipore), anti- phospho-eIF-2α (Ser51) and anti- eIF-2α (Cell Signaling) and control IgG (Santa Cruz Biotechnology).
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2

Antibody Panel for Protein Interactome Analysis

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The following antibodies were employed in this study: anti-ADAR2 (Sigma), anti-ACTIN (Promega), anti-V5 (Invitrogen), anti-SFPQ/PSF (Abcam), anti-ADAR1 (Santa Cruz Biotechnology), anti-NCL (Santa Cruz Biotechnology), Anti-P54/NONO (Bethyl), anti-hnRNP-C1/C2 (Santa Cruz Biotechnology), anti-GAPDH (Invitrogen), anti-T7 epitope tag (Millipore), anti-V5-tag magnetic beads (MBL), NiNTA beads (Qiagen).
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